Oral mucosal endotoxin tolerance induction in chronic periodontitis

Manoj Muthukuru, Ravi Jotwani, Christopher W Cutler

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

The oral mucosa is exposed to a high density and diversity of gram-positive and gram-negative bacteria, but very little is known about how immune homeostasis is maintained in this environment, particularly in the inflammatory disease chronic periodontitis (CP). The cells of the innate immune response recognize bacterial structures via the Toll-like receptors (TLR). This activates intracellular signaling and transcription of proteins essential for the induction of an adaptive immune response; however, if unregulated, it can lead to destructive inflammatory responses. Using single-immunoenzyme labeling, we show that the human oral mucosa (gingiva) is infiltrated by large numbers of TLR2+ and TLR4+ cells and that their numbers increase significantly in CP, relative to health (P < 0.05, Student's t test). We also show that the numbers of TLR2+ but not TLR4+ cells increase linearly with inflammation (r2 = 0.33, P < 0.05). Double-immunofluorescence analysis confirms that TLR2 is coexpressed by monocytes (MC)/macrophages (mφ) in situ. Further analysis of gingival tissues by quantitative real-time PCR, however, indicates that despite a threefold increase in the expression of interleukin-1β (IL-1β) mRNA during CP, there is significant (30-fold) downregulation of TLR2 mRNA (P < 0.05, Student's t test). Also showing similar trends are the levels of TLR4 (ninefold reduction), TLR5 (twofold reduction), and MD-2 (sevenfold reduction) mRNA in CP patients compared to healthy persons, while the level of CD14 was unchanged. In vitro studies with human MC indicate that MC respond to an initial stimulus of lipopolysaccharide (LPS) from Porphyromonas gingivalis (PgLPS) or Escherichia coli (EcLPS) by upregulation of TLR2 and TLR4 mRNA and protein; moreover, IL-1β mRNA is induced and tumor necrosis factor alpha (TNF-α), IL-10, IL-6, and IL-8 proteins are secreted. However, restimulation of MC with either PgLPS or EcLPS downregulates TLR2 and TLR4 mRNA and protein and IL-1β mRNA and induces a ca. 10-fold reduction in TNF-α secretion, suggesting the induction of endotoxin tolerance by either LPS. Less susceptible to tolerance than TNF-α were IL-6, IL-10, and IL-8. These studies suggest that certain components of the innate oral mucosal immune response, most notably TLRs and inflammatory cytokines, may become tolerized during sustained exposure to bacterial structures such as LPS and that this may be one mechanism used in the oral mucosa to attempt to regulate local immune responses.

Original languageEnglish (US)
Pages (from-to)687-694
Number of pages8
JournalInfection and Immunity
Volume73
Issue number2
DOIs
StatePublished - Feb 1 2005

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Chronic Periodontitis
Endotoxins
Messenger RNA
Monocytes
Mouth Mucosa
Interleukin-1
Lipopolysaccharides
Bacterial Structures
Tumor Necrosis Factor-alpha
Interleukin-8
Interleukin-10
Interleukin-6
Down-Regulation
Intracellular Signaling Peptides and Proteins
Students
Mucosal Immunity
Porphyromonas gingivalis
Proteins
Toll-Like Receptors
Gingiva

ASJC Scopus subject areas

  • Immunology

Cite this

Oral mucosal endotoxin tolerance induction in chronic periodontitis. / Muthukuru, Manoj; Jotwani, Ravi; Cutler, Christopher W.

In: Infection and Immunity, Vol. 73, No. 2, 01.02.2005, p. 687-694.

Research output: Contribution to journalArticle

Muthukuru, Manoj ; Jotwani, Ravi ; Cutler, Christopher W. / Oral mucosal endotoxin tolerance induction in chronic periodontitis. In: Infection and Immunity. 2005 ; Vol. 73, No. 2. pp. 687-694.
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