Organization of class I genes in the major histocompatibility complex of the C57BL/10 mouse

J. J. Devlin, A. L. Mellor, E. H. Weiss

Research output: Contribution to journalArticle

Abstract

We have isolated 26 different Class I genes from the C57BL/10 mouse. Overlapping cosmid clones have allowed us to organize these genes into three clusters, one mapping to the H-2K region (2 genes), one linking the H-2D and Qa2,3 regions (11 genes), and one mapping to the TL region (13 genes). The H-2K(bml) mutation seems to have been caused by gene conversion, involving the transfer of at least 13 but no more than 51 base pairs from a gene in the Qa2,3 region (the Q10 gene) to the H-2K gene. The Q10 gene is nonpolymorphic and appears to be transcribed in the liver; a protein encoded by this gene would have a truncated transmembrane domain and may be secreted. There are three homologous gene pairs in the Qa2,3 cluster suggesting that the number of genes in this region was expanded by the duplication of an ancestoral gene pair. These pairs are also homologous to the gene pair in the H-2K region; hence the H-2K region may have been generated by the translocation of a gene pair from the Qa2,3 region. Thus, this multigene family has apparently evolved by a series of gene duplication, translocation, and gene conversion events.

Original languageEnglish (US)
JournalFederation Proceedings
Volume43
Issue number6
StatePublished - Jan 1 1984

Fingerprint

MHC Class I Genes
Major Histocompatibility Complex
Inbred C57BL Mouse
Genes
Gene Conversion
Gene Duplication
Cosmids
Chromosome Mapping
Multigene Family
Base Pairing
Clone Cells

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Organization of class I genes in the major histocompatibility complex of the C57BL/10 mouse. / Devlin, J. J.; Mellor, A. L.; Weiss, E. H.

In: Federation Proceedings, Vol. 43, No. 6, 01.01.1984.

Research output: Contribution to journalArticle

Devlin, J. J. ; Mellor, A. L. ; Weiss, E. H. / Organization of class I genes in the major histocompatibility complex of the C57BL/10 mouse. In: Federation Proceedings. 1984 ; Vol. 43, No. 6.
@article{7ebfd2701ff347a2972e523acebcf5c1,
title = "Organization of class I genes in the major histocompatibility complex of the C57BL/10 mouse",
abstract = "We have isolated 26 different Class I genes from the C57BL/10 mouse. Overlapping cosmid clones have allowed us to organize these genes into three clusters, one mapping to the H-2K region (2 genes), one linking the H-2D and Qa2,3 regions (11 genes), and one mapping to the TL region (13 genes). The H-2K(bml) mutation seems to have been caused by gene conversion, involving the transfer of at least 13 but no more than 51 base pairs from a gene in the Qa2,3 region (the Q10 gene) to the H-2K gene. The Q10 gene is nonpolymorphic and appears to be transcribed in the liver; a protein encoded by this gene would have a truncated transmembrane domain and may be secreted. There are three homologous gene pairs in the Qa2,3 cluster suggesting that the number of genes in this region was expanded by the duplication of an ancestoral gene pair. These pairs are also homologous to the gene pair in the H-2K region; hence the H-2K region may have been generated by the translocation of a gene pair from the Qa2,3 region. Thus, this multigene family has apparently evolved by a series of gene duplication, translocation, and gene conversion events.",
author = "Devlin, {J. J.} and Mellor, {A. L.} and Weiss, {E. H.}",
year = "1984",
month = "1",
day = "1",
language = "English (US)",
volume = "43",
journal = "Federation Proceedings",
issn = "0014-9446",
number = "6",

}

TY - JOUR

T1 - Organization of class I genes in the major histocompatibility complex of the C57BL/10 mouse

AU - Devlin, J. J.

AU - Mellor, A. L.

AU - Weiss, E. H.

PY - 1984/1/1

Y1 - 1984/1/1

N2 - We have isolated 26 different Class I genes from the C57BL/10 mouse. Overlapping cosmid clones have allowed us to organize these genes into three clusters, one mapping to the H-2K region (2 genes), one linking the H-2D and Qa2,3 regions (11 genes), and one mapping to the TL region (13 genes). The H-2K(bml) mutation seems to have been caused by gene conversion, involving the transfer of at least 13 but no more than 51 base pairs from a gene in the Qa2,3 region (the Q10 gene) to the H-2K gene. The Q10 gene is nonpolymorphic and appears to be transcribed in the liver; a protein encoded by this gene would have a truncated transmembrane domain and may be secreted. There are three homologous gene pairs in the Qa2,3 cluster suggesting that the number of genes in this region was expanded by the duplication of an ancestoral gene pair. These pairs are also homologous to the gene pair in the H-2K region; hence the H-2K region may have been generated by the translocation of a gene pair from the Qa2,3 region. Thus, this multigene family has apparently evolved by a series of gene duplication, translocation, and gene conversion events.

AB - We have isolated 26 different Class I genes from the C57BL/10 mouse. Overlapping cosmid clones have allowed us to organize these genes into three clusters, one mapping to the H-2K region (2 genes), one linking the H-2D and Qa2,3 regions (11 genes), and one mapping to the TL region (13 genes). The H-2K(bml) mutation seems to have been caused by gene conversion, involving the transfer of at least 13 but no more than 51 base pairs from a gene in the Qa2,3 region (the Q10 gene) to the H-2K gene. The Q10 gene is nonpolymorphic and appears to be transcribed in the liver; a protein encoded by this gene would have a truncated transmembrane domain and may be secreted. There are three homologous gene pairs in the Qa2,3 cluster suggesting that the number of genes in this region was expanded by the duplication of an ancestoral gene pair. These pairs are also homologous to the gene pair in the H-2K region; hence the H-2K region may have been generated by the translocation of a gene pair from the Qa2,3 region. Thus, this multigene family has apparently evolved by a series of gene duplication, translocation, and gene conversion events.

UR - http://www.scopus.com/inward/record.url?scp=0021637771&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021637771&partnerID=8YFLogxK

M3 - Article

VL - 43

JO - Federation Proceedings

JF - Federation Proceedings

SN - 0014-9446

IS - 6

ER -