Organization of the bovine gene encoding the endothelial nitric oxide synthase

Richard C. Venema, Ken'ichi Nishida, R. Wayne Alexander, David G. Harrison, T. J. Murphy

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

The bovine endothelial nitric oxide synthase gene plus 2.9 kilobases of 5′-flanking sequence has been isolated and characterized. The gene spans 20 kilobases and contains 26 exons and 25 introns. Two transcription start sites have been determined by primer extension analysis which are located 170 and 240 base pairs upstream, respectively, from the methionine translational initiation codon. Evidence supporting the upstream boundary region for transcriptional initiation was also obtained by reverse transcription-polymerase chain reaction. The 5′-flanking region lacks a typical TATA box but contains numerous putative transcription factor binding sites. These include consensus sequences for an AP-1 site, an NF-1 site, a tumor necrosis factor responsive element, two sterol regulatory elements, 3 acute-phase response elements, 6 GATA motifs, 16 CACCC boxes, 5 Sp1 sites, 15 estrogen half-palindromic motifs, and 9 fluid shear stress-responsive elements. The isolated gene promoter directs basal transcription of a luciferase reporter gene when transiently transfected into bovine aortic endothelial cells. High sequence homology of the promoter region to the human endothelial nitric oxide synthase gene promoter (75% nucleotide identity in 1.6 kilobases of 5′-flanking sequence) suggests evolutionary conservation of transcriptional regulation. Isolation and characterization of the bovine endothelial nitric oxide synthase gene should facilitate further investigation of mechanisms by which gene expression is regulated.

Original languageEnglish (US)
Pages (from-to)413-420
Number of pages8
JournalBBA - Gene Structure and Expression
Volume1218
Issue number3
DOIs
StatePublished - Aug 2 1994

Fingerprint

Gene encoding
Nitric Oxide Synthase Type III
Genes
5' Flanking Region
Transcription
TATA Box
Acute-Phase Reaction
Initiator Codon
Transcription Initiation Site
Transcription Factor AP-1
Consensus Sequence
Response Elements
Sterols
Sequence Homology
Luciferases
Reporter Genes
Genetic Promoter Regions
Base Pairing
Methionine
Polymerase chain reaction

Keywords

  • (Bovine aortic endothelial cell)
  • Gene structure
  • Nitric oxide synthase
  • Promoter deletional analysis
  • Transcriptional regulation

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

Cite this

Venema, R. C., Nishida, K., Alexander, R. W., Harrison, D. G., & Murphy, T. J. (1994). Organization of the bovine gene encoding the endothelial nitric oxide synthase. BBA - Gene Structure and Expression, 1218(3), 413-420. https://doi.org/10.1016/0167-4781(94)90195-3

Organization of the bovine gene encoding the endothelial nitric oxide synthase. / Venema, Richard C.; Nishida, Ken'ichi; Alexander, R. Wayne; Harrison, David G.; Murphy, T. J.

In: BBA - Gene Structure and Expression, Vol. 1218, No. 3, 02.08.1994, p. 413-420.

Research output: Contribution to journalArticle

Venema, RC, Nishida, K, Alexander, RW, Harrison, DG & Murphy, TJ 1994, 'Organization of the bovine gene encoding the endothelial nitric oxide synthase', BBA - Gene Structure and Expression, vol. 1218, no. 3, pp. 413-420. https://doi.org/10.1016/0167-4781(94)90195-3
Venema, Richard C. ; Nishida, Ken'ichi ; Alexander, R. Wayne ; Harrison, David G. ; Murphy, T. J. / Organization of the bovine gene encoding the endothelial nitric oxide synthase. In: BBA - Gene Structure and Expression. 1994 ; Vol. 1218, No. 3. pp. 413-420.
@article{c3029c80254340418becaa24bddf9458,
title = "Organization of the bovine gene encoding the endothelial nitric oxide synthase",
abstract = "The bovine endothelial nitric oxide synthase gene plus 2.9 kilobases of 5′-flanking sequence has been isolated and characterized. The gene spans 20 kilobases and contains 26 exons and 25 introns. Two transcription start sites have been determined by primer extension analysis which are located 170 and 240 base pairs upstream, respectively, from the methionine translational initiation codon. Evidence supporting the upstream boundary region for transcriptional initiation was also obtained by reverse transcription-polymerase chain reaction. The 5′-flanking region lacks a typical TATA box but contains numerous putative transcription factor binding sites. These include consensus sequences for an AP-1 site, an NF-1 site, a tumor necrosis factor responsive element, two sterol regulatory elements, 3 acute-phase response elements, 6 GATA motifs, 16 CACCC boxes, 5 Sp1 sites, 15 estrogen half-palindromic motifs, and 9 fluid shear stress-responsive elements. The isolated gene promoter directs basal transcription of a luciferase reporter gene when transiently transfected into bovine aortic endothelial cells. High sequence homology of the promoter region to the human endothelial nitric oxide synthase gene promoter (75{\%} nucleotide identity in 1.6 kilobases of 5′-flanking sequence) suggests evolutionary conservation of transcriptional regulation. Isolation and characterization of the bovine endothelial nitric oxide synthase gene should facilitate further investigation of mechanisms by which gene expression is regulated.",
keywords = "(Bovine aortic endothelial cell), Gene structure, Nitric oxide synthase, Promoter deletional analysis, Transcriptional regulation",
author = "Venema, {Richard C.} and Ken'ichi Nishida and Alexander, {R. Wayne} and Harrison, {David G.} and Murphy, {T. J.}",
year = "1994",
month = "8",
day = "2",
doi = "10.1016/0167-4781(94)90195-3",
language = "English (US)",
volume = "1218",
pages = "413--420",
journal = "Biochimica et Biophysica Acta - Gene Structure and Expression",
issn = "0167-4781",
publisher = "Elsevier BV",
number = "3",

}

TY - JOUR

T1 - Organization of the bovine gene encoding the endothelial nitric oxide synthase

AU - Venema, Richard C.

AU - Nishida, Ken'ichi

AU - Alexander, R. Wayne

AU - Harrison, David G.

AU - Murphy, T. J.

PY - 1994/8/2

Y1 - 1994/8/2

N2 - The bovine endothelial nitric oxide synthase gene plus 2.9 kilobases of 5′-flanking sequence has been isolated and characterized. The gene spans 20 kilobases and contains 26 exons and 25 introns. Two transcription start sites have been determined by primer extension analysis which are located 170 and 240 base pairs upstream, respectively, from the methionine translational initiation codon. Evidence supporting the upstream boundary region for transcriptional initiation was also obtained by reverse transcription-polymerase chain reaction. The 5′-flanking region lacks a typical TATA box but contains numerous putative transcription factor binding sites. These include consensus sequences for an AP-1 site, an NF-1 site, a tumor necrosis factor responsive element, two sterol regulatory elements, 3 acute-phase response elements, 6 GATA motifs, 16 CACCC boxes, 5 Sp1 sites, 15 estrogen half-palindromic motifs, and 9 fluid shear stress-responsive elements. The isolated gene promoter directs basal transcription of a luciferase reporter gene when transiently transfected into bovine aortic endothelial cells. High sequence homology of the promoter region to the human endothelial nitric oxide synthase gene promoter (75% nucleotide identity in 1.6 kilobases of 5′-flanking sequence) suggests evolutionary conservation of transcriptional regulation. Isolation and characterization of the bovine endothelial nitric oxide synthase gene should facilitate further investigation of mechanisms by which gene expression is regulated.

AB - The bovine endothelial nitric oxide synthase gene plus 2.9 kilobases of 5′-flanking sequence has been isolated and characterized. The gene spans 20 kilobases and contains 26 exons and 25 introns. Two transcription start sites have been determined by primer extension analysis which are located 170 and 240 base pairs upstream, respectively, from the methionine translational initiation codon. Evidence supporting the upstream boundary region for transcriptional initiation was also obtained by reverse transcription-polymerase chain reaction. The 5′-flanking region lacks a typical TATA box but contains numerous putative transcription factor binding sites. These include consensus sequences for an AP-1 site, an NF-1 site, a tumor necrosis factor responsive element, two sterol regulatory elements, 3 acute-phase response elements, 6 GATA motifs, 16 CACCC boxes, 5 Sp1 sites, 15 estrogen half-palindromic motifs, and 9 fluid shear stress-responsive elements. The isolated gene promoter directs basal transcription of a luciferase reporter gene when transiently transfected into bovine aortic endothelial cells. High sequence homology of the promoter region to the human endothelial nitric oxide synthase gene promoter (75% nucleotide identity in 1.6 kilobases of 5′-flanking sequence) suggests evolutionary conservation of transcriptional regulation. Isolation and characterization of the bovine endothelial nitric oxide synthase gene should facilitate further investigation of mechanisms by which gene expression is regulated.

KW - (Bovine aortic endothelial cell)

KW - Gene structure

KW - Nitric oxide synthase

KW - Promoter deletional analysis

KW - Transcriptional regulation

UR - http://www.scopus.com/inward/record.url?scp=0028068430&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028068430&partnerID=8YFLogxK

U2 - 10.1016/0167-4781(94)90195-3

DO - 10.1016/0167-4781(94)90195-3

M3 - Article

C2 - 7519447

AN - SCOPUS:0028068430

VL - 1218

SP - 413

EP - 420

JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

SN - 0167-4781

IS - 3

ER -