Organization of the flaFG gene cluster and identification of two additional genes involved in flagellum biogenesis in Caulobacter crescentus

Patricia V Schoenlein, L. S. Gallman, B. Ely

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In Caulobacter crescentus, mutations have been isolated in more than 30 flagellar genes (fla, flb, and flg) which are required in the cell cycle event of flagellum biogenesis. The flaF and flaG mutations and two newly identified mutations, flbT and flbA (P. V. Schoenlein and B. Ely, J. Bacteriol. 171: 000-000, 1989), have been localized to the flaFG region. In this study, the genetic and physical organization of this region was analyzed, using the cloned 4.0-kilobase flaFG region in the recombinant plasmid pPLG727. Plasmid pPLG727 complemented flaF, flaG, flbA, and flbT mutations. Further complementation studies with pPLG727 derivatives indicated that flaF and flbT are unique but overlapping transcription units, whereas flbA and flaG constitute a single transcription unit. To determine the direction of transcription of the putative flbA-flaG operon, the promoterless chloramphenicol transacetylase gene was inserted into various positions in the flbA-flaG region, and merodiploid strains containing these transcriptional fusions were assayed for gene function and expression of chloramphenicol resistance. These results showed that transcription proceeds from flbA to flaG. To confirm the complementation analysis, Southern analyses were performed on chromosomal DNAs isolated from strains containing insertion and deletion mutations. Taken together, these studies defined the relative gene order at one end of the flaYG flagellar gene cluster as flgL-flaF-flbT-flbA-flaG.

Original languageEnglish (US)
Pages (from-to)1544-1553
Number of pages10
JournalJournal of Bacteriology
Volume171
Issue number3
DOIs
StatePublished - Jan 1 1989
Externally publishedYes

Fingerprint

Caulobacter crescentus
Flagella
Multigene Family
Mutation
Genes
Plasmids
INDEL Mutation
Chloramphenicol Resistance
Chloramphenicol O-Acetyltransferase
Gene Order
Operon
Cell Cycle
Gene Expression
DNA

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Organization of the flaFG gene cluster and identification of two additional genes involved in flagellum biogenesis in Caulobacter crescentus. / Schoenlein, Patricia V; Gallman, L. S.; Ely, B.

In: Journal of Bacteriology, Vol. 171, No. 3, 01.01.1989, p. 1544-1553.

Research output: Contribution to journalArticle

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