Origin and T Cell Receptor Diversity of Foxp3+CD4+CD25+ T Cells

Rafal Pacholczyk, Hanna Ignatowicz, Piotr Kraj, Leszek Ignatowicz

Research output: Contribution to journalArticle

229 Scopus citations

Abstract

Foxp3+CD4+CD25+ regulatory T cells can differentiate from Foxp3-CD4+ medullary thymocytes and Foxp3-CD4+ naive T cells. However, the impact of these two processes on size and composition of the peripheral repertoire of regulatory T cells is unclear. Here we followed the fate of individual Foxp3+CD4+CD25+ thymocytes and T cells in vivo in T cell receptor (TCR) transgenic mice that express a restricted but polyclonal repertoire of TCRs. By utilizing high-throughput single-cell analysis, we showed that Foxp3+CD4+ peripheral T cells were derived from thymic precursors that expressed a different TCRs than Foxp3-CD4+ medullary thymocytes and Foxp3-CD4+ T cells. Furthermore, the diversity of TCRs on Foxp3+CD4+ regulatory T cells exceeded the diversity of TCRs on Foxp3-CD4+ naive T cells, even in mice that lack expression of tissue-specific antigens. Our results imply that higher TCR diversity on Foxp3+ regulatory T cells helps these cells to match the specificities of autoreactive and naive T cells.

Original languageEnglish (US)
Pages (from-to)249-259
Number of pages11
JournalImmunity
Volume25
Issue number2
DOIs
StatePublished - Aug 1 2006

Keywords

  • CELLIMMUNO

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

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