Osmometric behavior of mouse oocytes in the presence of different intracellular sugars

Pragati Bhowmick, Ali Eroglu, Diane L. Wright, Mehmet Toner, Thomas L. Toth

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

In order to successfully cryopreserve oocytes using low concentrations of intracellular sugars, it is important to characterize their osmotic response in the presence of these intracellular sugars. In the present study, murine (B6D2F1) oocytes were microinjected with 0.8 M glucose, trehalose or stachyose solutions to achieve an intracellular concentration equivalent to 0.1 M, and then exposed to hypertonic solutions of increasing strength by supplementing an isotonic solution with 0.2, 0.3, 0.5, and 0.7 M of trehalose. Analysis of volumetric response of microinjected oocytes showed that the oocytes behaved as ideal osmometers in the presence of intracellular sugars and satisfied the Boyle van't Hoff relationship. Extrapolation of the osmotically inactive fraction (V̄b) from the Boyle van't Hoff relationship yielded values of 0.188 ± 0.028, 0.212 ± 0.042, 0.197 ± 0.044, and 0.211 ± 0.042 for control, glucose, trehalose and stachyose-injected oocytes, respectively. The present data revealing osmometric behavior of mouse oocytes in the presence of different intracellular sugars are important for the optimization of cryopreservation protocols using sugars.

Original languageEnglish (US)
Pages (from-to)183-187
Number of pages5
JournalCryobiology
Volume45
Issue number2
DOIs
StatePublished - Oct 1 2002
Externally publishedYes

Keywords

  • Glucose
  • Osmometric behavior
  • Osmotically inactive fraction
  • Stachyose
  • Trehalose

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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