Osteoblast-derived cells express functional glucose-dependent insulinotropic peptide receptors

R. J. Bollag, Q. Zhong, P. Phillips, L. Min, L. Zhong, R. Cameron, A. L. Mulloy, H. Rasmussen, F. Qin, K. H. Ding, C. M. Isales

Research output: Contribution to journalArticle

166 Citations (Scopus)

Abstract

Glucose-dependent insulinotropic peptide (GIP) is a 42-amino acid peptide synthesized and secreted from endocrine cells in the small intestine. The role of GIP in coupling nutrient intake and insulin secretion, the incretin effect, is well known. We report that GIP receptor messenger RNA and protein are present in normal bone and osteoblast-like cell lines, and that high affinity receptors for GIP can be demonstrated by [125I]GIP binding studies. When applied to osteoblast-like cells (SaOS2), GIP stimulated increases in cellular cAMP content and intracellular calcium, with both responses being dose dependent. Moreover, administration of GIP results in elevated expression of collagen type I messenger RNA as well as an increase in alkaline phosphatase activity. Both of these effects reflect anabolic actions of presumptive osteoblasts. These results provide the first evidence that GIP receptors are present in bone and osteoblast-like cells and that GIP modulates the function of these cells.

Original languageEnglish (US)
Pages (from-to)1228-1235
Number of pages8
JournalEndocrinology
Volume141
Issue number3
DOIs
StatePublished - Jan 1 2000

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Gastric Inhibitory Polypeptide
Peptide Receptors
Osteoblasts
Incretins
Bone and Bones
Messenger RNA
Endocrine Cells
Collagen Type I
Small Intestine
Alkaline Phosphatase
Insulin
Calcium
Amino Acids

ASJC Scopus subject areas

  • Endocrinology

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Osteoblast-derived cells express functional glucose-dependent insulinotropic peptide receptors. / Bollag, R. J.; Zhong, Q.; Phillips, P.; Min, L.; Zhong, L.; Cameron, R.; Mulloy, A. L.; Rasmussen, H.; Qin, F.; Ding, K. H.; Isales, C. M.

In: Endocrinology, Vol. 141, No. 3, 01.01.2000, p. 1228-1235.

Research output: Contribution to journalArticle

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AU - Phillips, P.

AU - Min, L.

AU - Zhong, L.

AU - Cameron, R.

AU - Mulloy, A. L.

AU - Rasmussen, H.

AU - Qin, F.

AU - Ding, K. H.

AU - Isales, C. M.

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N2 - Glucose-dependent insulinotropic peptide (GIP) is a 42-amino acid peptide synthesized and secreted from endocrine cells in the small intestine. The role of GIP in coupling nutrient intake and insulin secretion, the incretin effect, is well known. We report that GIP receptor messenger RNA and protein are present in normal bone and osteoblast-like cell lines, and that high affinity receptors for GIP can be demonstrated by [125I]GIP binding studies. When applied to osteoblast-like cells (SaOS2), GIP stimulated increases in cellular cAMP content and intracellular calcium, with both responses being dose dependent. Moreover, administration of GIP results in elevated expression of collagen type I messenger RNA as well as an increase in alkaline phosphatase activity. Both of these effects reflect anabolic actions of presumptive osteoblasts. These results provide the first evidence that GIP receptors are present in bone and osteoblast-like cells and that GIP modulates the function of these cells.

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