Osteoconductive Effects of Vinyl Styrene Microbeads in Rat Calvarial Defects

Khalid Mohamed Marzouk, Ahmed Y. Gamal, Akram A. Al-Awady, Mohamed M.H. Sharawy

Research output: Contribution to journalArticle

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Abstract

Purpose: The aim of the present study was to evaluate the use of the nonresorbable vinyl styrene microbeads (VSM) alloplast as a delivery vehicle for platelet-derived growth factor (PDGF-BB) in rat calvarial critical size defects. Materials and Methods: Seventy-three Long-Evans male rats were divided into 4 groups; a negative control, vinyl styrene microbeads (VSM), PDGF-BB, and VSM plus PDGF-BB. Critical size calvarial defects were carried out and isolated with membranes sandwiching the defects with their fillers. Animals were sacrificed after 2, 4, and 16 weeks classifying each group into 3 subgroups. Calvarial specimens were radiographed for evaluation of regenerated bone volume and densitometry histogram analysis. Specimens were divided mid-sagittally and stained with hematoxylin and eosin (H&E) and trichrome stain for qualitative and histomorphometric analysis using an image analysis software. Results: The VSM groups showed statistically higher defect fills than the VSM-free groups at all sacrifice times except for the VSM/PDGF group that showed this difference after 2 weeks in relation to the PDGF group and the negative control after 4 and 16 weeks. For the radiographic analysis, the VSM/PDGF group showed the lowest bone volume compared with the other groups except when it was compared with the 4 weeks VSM group. In contrast, the PDGF showed the highest bone volumes at all sacrifice times that were only significant when compared with the 4 weeks VSM group and the 4 and 16 weeks VSM/PDGF group. Conclusions: VSM enhances bone defect fill whereas the VSM/PDGF-BB is not able to improve bone regeneration capacity when compared with VSM alone.

Original languageEnglish (US)
Pages (from-to)1508-1516
Number of pages9
JournalJournal of Oral and Maxillofacial Surgery
Volume65
Issue number8
DOIs
StatePublished - Aug 1 2007

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Styrene
Microspheres
Bone and Bones
Inbred BB Rats
Long Evans Rats
Bone Regeneration
Densitometry
Hematoxylin
Eosine Yellowish-(YS)
Software

ASJC Scopus subject areas

  • Surgery
  • Oral Surgery
  • Otorhinolaryngology

Cite this

Osteoconductive Effects of Vinyl Styrene Microbeads in Rat Calvarial Defects. / Marzouk, Khalid Mohamed; Gamal, Ahmed Y.; Al-Awady, Akram A.; Sharawy, Mohamed M.H.

In: Journal of Oral and Maxillofacial Surgery, Vol. 65, No. 8, 01.08.2007, p. 1508-1516.

Research output: Contribution to journalArticle

Marzouk, Khalid Mohamed ; Gamal, Ahmed Y. ; Al-Awady, Akram A. ; Sharawy, Mohamed M.H. / Osteoconductive Effects of Vinyl Styrene Microbeads in Rat Calvarial Defects. In: Journal of Oral and Maxillofacial Surgery. 2007 ; Vol. 65, No. 8. pp. 1508-1516.
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abstract = "Purpose: The aim of the present study was to evaluate the use of the nonresorbable vinyl styrene microbeads (VSM) alloplast as a delivery vehicle for platelet-derived growth factor (PDGF-BB) in rat calvarial critical size defects. Materials and Methods: Seventy-three Long-Evans male rats were divided into 4 groups; a negative control, vinyl styrene microbeads (VSM), PDGF-BB, and VSM plus PDGF-BB. Critical size calvarial defects were carried out and isolated with membranes sandwiching the defects with their fillers. Animals were sacrificed after 2, 4, and 16 weeks classifying each group into 3 subgroups. Calvarial specimens were radiographed for evaluation of regenerated bone volume and densitometry histogram analysis. Specimens were divided mid-sagittally and stained with hematoxylin and eosin (H&E) and trichrome stain for qualitative and histomorphometric analysis using an image analysis software. Results: The VSM groups showed statistically higher defect fills than the VSM-free groups at all sacrifice times except for the VSM/PDGF group that showed this difference after 2 weeks in relation to the PDGF group and the negative control after 4 and 16 weeks. For the radiographic analysis, the VSM/PDGF group showed the lowest bone volume compared with the other groups except when it was compared with the 4 weeks VSM group. In contrast, the PDGF showed the highest bone volumes at all sacrifice times that were only significant when compared with the 4 weeks VSM group and the 4 and 16 weeks VSM/PDGF group. Conclusions: VSM enhances bone defect fill whereas the VSM/PDGF-BB is not able to improve bone regeneration capacity when compared with VSM alone.",
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