Overexpression of human catalase inhibits proliferation and promotes apoptosis in vascular smooth muscle cells

Michael R. Brown, Francis J. Miller, Wei Gen Li, Andy N. Ellingson, Jonathan D. Mozena, Papri Chatterjee, John F. Engelhardt, Ralf M. Zwacka, Larry W. Oberley, Xiang Fang, Arthur A. Spector, Neal L. Weintraub

Research output: Contribution to journalArticle

171 Citations (Scopus)

Abstract

The role of reactive oxygen species, such as superoxide anions (O 2 · - ) and hydrogen peroxide (H 2 O 2 ), in modulating vascular smooth muscle cell proliferation and viability is controversial. To investigate the role of endogenously produced H 2 O 2 , rat aortic smooth muscle cells were infected with adenoviral vectors containing cDNA for human catalase (AdCat) or a control gene, β-galactosidase (AdLacZ). Infection with AdCat resulted in dose-dependent increases in intracellular catalase protein, which was predominantly localized to peroxisomes. After infection with 100 multiplicity of infection (MOI) of AdCat, cellular catalase activity was increased by 50- to 100-fold, and intracellular H 2 O 2 concentration was reduced, as compared with control. Infection with AdCat reduced [ 3 H]thymidine uptake, an index of DNA synthesis, in cells maintained in medium supplemented with 2% serum (0.37 ± 0.09 disintegrations per minute per cell [AdLacZ] versus 0.22 ± 0.08 disintegrations per minute per cell [AdCat], P<0.05). Five days after infection with 100 MOI of AdCat, cell numbers were reduced as compared with noninfected or AdLacZ-infected cells (157 780 ± 8413 [AdCat], P<0.05 versus 233 700 ± 3032 [noninfected] or 222 410 ± 5332 [AdLacZ]). Furthermore, the number of apoptotic cells was increased 5-fold after infection with 100 MOI of AdCat as compared with control. Infection with AdCat resulted in induction of cyclooxygenase (COX)-2, and treatment with a COX-2 inhibitor overcame the AdCat-induced reduction in cell numbers. These findings indicate that overexpression of catalase inhibited smooth muscle proliferation while increasing the rate of apoptosis, possibly through a COX-2-dependent mechanism. Our results suggest that endogenously produced H 2 O 2 importantly modulates survival and proliferation of vascular smooth muscle cells.

Original languageEnglish (US)
Pages (from-to)524-533
Number of pages10
JournalCirculation Research
Volume85
Issue number6
DOIs
StatePublished - Sep 17 1999

Fingerprint

Vascular Smooth Muscle
Catalase
Smooth Muscle Myocytes
Apoptosis
Infection
Cell Count
Cyclooxygenase 2
Galactosidases
Peroxisomes
Cyclooxygenase 2 Inhibitors
Superoxides
Thymidine
Hydrogen Peroxide
Smooth Muscle
Reactive Oxygen Species
Cell Survival
Complementary DNA
Cell Proliferation
Survival
DNA

Keywords

  • Apoptosis
  • Catalase
  • Cell proliferation
  • Hydrogen peroxide
  • Vascular smooth muscle cell

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Overexpression of human catalase inhibits proliferation and promotes apoptosis in vascular smooth muscle cells. / Brown, Michael R.; Miller, Francis J.; Li, Wei Gen; Ellingson, Andy N.; Mozena, Jonathan D.; Chatterjee, Papri; Engelhardt, John F.; Zwacka, Ralf M.; Oberley, Larry W.; Fang, Xiang; Spector, Arthur A.; Weintraub, Neal L.

In: Circulation Research, Vol. 85, No. 6, 17.09.1999, p. 524-533.

Research output: Contribution to journalArticle

Brown, MR, Miller, FJ, Li, WG, Ellingson, AN, Mozena, JD, Chatterjee, P, Engelhardt, JF, Zwacka, RM, Oberley, LW, Fang, X, Spector, AA & Weintraub, NL 1999, 'Overexpression of human catalase inhibits proliferation and promotes apoptosis in vascular smooth muscle cells', Circulation Research, vol. 85, no. 6, pp. 524-533. https://doi.org/10.1161/01.RES.85.6.524
Brown, Michael R. ; Miller, Francis J. ; Li, Wei Gen ; Ellingson, Andy N. ; Mozena, Jonathan D. ; Chatterjee, Papri ; Engelhardt, John F. ; Zwacka, Ralf M. ; Oberley, Larry W. ; Fang, Xiang ; Spector, Arthur A. ; Weintraub, Neal L. / Overexpression of human catalase inhibits proliferation and promotes apoptosis in vascular smooth muscle cells. In: Circulation Research. 1999 ; Vol. 85, No. 6. pp. 524-533.
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abstract = "The role of reactive oxygen species, such as superoxide anions (O 2 · - ) and hydrogen peroxide (H 2 O 2 ), in modulating vascular smooth muscle cell proliferation and viability is controversial. To investigate the role of endogenously produced H 2 O 2 , rat aortic smooth muscle cells were infected with adenoviral vectors containing cDNA for human catalase (AdCat) or a control gene, β-galactosidase (AdLacZ). Infection with AdCat resulted in dose-dependent increases in intracellular catalase protein, which was predominantly localized to peroxisomes. After infection with 100 multiplicity of infection (MOI) of AdCat, cellular catalase activity was increased by 50- to 100-fold, and intracellular H 2 O 2 concentration was reduced, as compared with control. Infection with AdCat reduced [ 3 H]thymidine uptake, an index of DNA synthesis, in cells maintained in medium supplemented with 2{\%} serum (0.37 ± 0.09 disintegrations per minute per cell [AdLacZ] versus 0.22 ± 0.08 disintegrations per minute per cell [AdCat], P<0.05). Five days after infection with 100 MOI of AdCat, cell numbers were reduced as compared with noninfected or AdLacZ-infected cells (157 780 ± 8413 [AdCat], P<0.05 versus 233 700 ± 3032 [noninfected] or 222 410 ± 5332 [AdLacZ]). Furthermore, the number of apoptotic cells was increased 5-fold after infection with 100 MOI of AdCat as compared with control. Infection with AdCat resulted in induction of cyclooxygenase (COX)-2, and treatment with a COX-2 inhibitor overcame the AdCat-induced reduction in cell numbers. These findings indicate that overexpression of catalase inhibited smooth muscle proliferation while increasing the rate of apoptosis, possibly through a COX-2-dependent mechanism. Our results suggest that endogenously produced H 2 O 2 importantly modulates survival and proliferation of vascular smooth muscle cells.",
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