TY - JOUR
T1 - Oxidative stress induced apoptosis of human lung carcinoma (A549) cells by a novel copper nanorod formulation
AU - Thounaojam, Menaka C.
AU - Jadeja, Ravirajsinh N.
AU - Valodkar, Mayur
AU - Nagar, Padamanabhi S.
AU - Devkar, Ranjitsinh V.
AU - Thakore, Sonal
N1 - Funding Information:
The authors are grateful to the University Grants Commission, New Delhi for financial assistance. Special thanks to Dr. Geeta. S. Padate, Head, Department of Zoology for her encouragement and necessary permission.
Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2011/11
Y1 - 2011/11
N2 - This study elucidates the process of synthesis of copper (Cu) nanorods using almond skin extract as stabilizing cum capping agent. These nanorods were (about 200. nm long and 40. nm wide) characterized by transmission electron microscopy (TEM). Further, cytotoxicity potential of these nanorods was evaluated in A549 cells (Human lung carcinoma cell line) via cell viability assay and extracellular lactate dehydrogenase (LDH) release. Also, reduced glutathione (GSH), lipid peroxidation (LPO), cellular oxidative stress (Rhodamine 123 florescence) and apoptosis (Annexin V FITC/Propidium iodide staining) were also investigated in control and treated cells. Results indicated that Cu nanorods induced apoptotic death of cancer cells by induction of oxidative stress, depletion of cellular antioxidants and mitochondrial dysfunction. This study reports a novel process of synthesis of almond skin extract capped Cu nanorods and its potential as an anticancer agent against A549 lung carcinoma cells.
AB - This study elucidates the process of synthesis of copper (Cu) nanorods using almond skin extract as stabilizing cum capping agent. These nanorods were (about 200. nm long and 40. nm wide) characterized by transmission electron microscopy (TEM). Further, cytotoxicity potential of these nanorods was evaluated in A549 cells (Human lung carcinoma cell line) via cell viability assay and extracellular lactate dehydrogenase (LDH) release. Also, reduced glutathione (GSH), lipid peroxidation (LPO), cellular oxidative stress (Rhodamine 123 florescence) and apoptosis (Annexin V FITC/Propidium iodide staining) were also investigated in control and treated cells. Results indicated that Cu nanorods induced apoptotic death of cancer cells by induction of oxidative stress, depletion of cellular antioxidants and mitochondrial dysfunction. This study reports a novel process of synthesis of almond skin extract capped Cu nanorods and its potential as an anticancer agent against A549 lung carcinoma cells.
KW - Almond skin extract
KW - Anticancer
KW - Copper nanorods
KW - Cytotoxicity
KW - Oxidative stress
UR - http://www.scopus.com/inward/record.url?scp=80053560228&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80053560228&partnerID=8YFLogxK
U2 - 10.1016/j.fct.2011.07.055
DO - 10.1016/j.fct.2011.07.055
M3 - Article
C2 - 21820027
AN - SCOPUS:80053560228
SN - 0278-6915
VL - 49
SP - 2990
EP - 2996
JO - Food and Chemical Toxicology
JF - Food and Chemical Toxicology
IS - 11
ER -
