Oxidative stress induced apoptosis of human lung carcinoma (A549) cells by a novel copper nanorod formulation

Menaka C. Thounaojam, Ravirajsinh N. Jadeja, Mayur Valodkar, Padamanabhi S. Nagar, Ranjitsinh V. Devkar, Sonal Thakore

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

This study elucidates the process of synthesis of copper (Cu) nanorods using almond skin extract as stabilizing cum capping agent. These nanorods were (about 200. nm long and 40. nm wide) characterized by transmission electron microscopy (TEM). Further, cytotoxicity potential of these nanorods was evaluated in A549 cells (Human lung carcinoma cell line) via cell viability assay and extracellular lactate dehydrogenase (LDH) release. Also, reduced glutathione (GSH), lipid peroxidation (LPO), cellular oxidative stress (Rhodamine 123 florescence) and apoptosis (Annexin V FITC/Propidium iodide staining) were also investigated in control and treated cells. Results indicated that Cu nanorods induced apoptotic death of cancer cells by induction of oxidative stress, depletion of cellular antioxidants and mitochondrial dysfunction. This study reports a novel process of synthesis of almond skin extract capped Cu nanorods and its potential as an anticancer agent against A549 lung carcinoma cells.

Original languageEnglish (US)
Pages (from-to)2990-2996
Number of pages7
JournalFood and Chemical Toxicology
Volume49
Issue number11
DOIs
StatePublished - Nov 1 2011
Externally publishedYes

Keywords

  • Almond skin extract
  • Anticancer
  • Copper nanorods
  • Cytotoxicity
  • Oxidative stress

ASJC Scopus subject areas

  • Food Science
  • Toxicology

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