P-selectin glycoprotein ligand-1 is highly expressed on ly-6Chi monocytes and a major determinant for ly-6Chi monocyte recruitment to sites of atherosclerosis in mice

Guangyu An, Huan Wang, Rong Tang, Tadayuki Yago, J. Michael McDaniel, Samuel McGee, Yuqing Huo, Lijun Xia

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

Background - Ly-6C monocytes are key contributors to atherosclerosis in mice. However, the manner in which Ly-6C monocytes selectively accumulate in atherosclerotic lesions is largely unknown. Monocyte homing to sites of atherosclerosis is primarily initiated by rolling on P- and E-selectin expressed on endothelium. We hypothesize that P-selectin glycoprotein ligand-1 (PSGL-1), the common ligand of P- and E-selectin on leukocytes, contributes to the preferential homing of Ly-6C monocytes to atherosclerotic lesions. Methods and Results - To test this hypothesis, we examined the expression and function of PSGL-1 on Ly-6C and Ly-6C monocytes from wild-type mice, ApoE mice, and mice lacking both ApoE and PSGL-1 genes (ApoE/PSGL-1). We found that Ly-6C monocytes expressed a higher level of PSGL-1 and had enhanced binding to fluid-phase P- and E-selectin compared with Ly-6C monocytes. Under in vitro flow conditions, more Ly-6C monocytes rolled on P-, E-, and L-selectin at slower velocities than Ly-6C cells. In an ex vivo perfused carotid artery model, Ly-6C monocytes interacted preferentially with atherosclerotic endothelium compared with Ly-6C monocytes in a PSGL-1-dependent manner. In vivo, ApoE mice lacking PSGL-1 had impaired Ly-6C monocyte recruitment to atherosclerotic lesions. Moreover, ApoE/PSGL-1 mice exhibited significantly reduced monocyte infiltration in wire injury-induced neointima and in atherosclerotic lesions. ApoE/PSGL-1 mice also developed smaller neointima and atherosclerotic plaques. Conclusions - These data indicate that PSGL-1 is a new marker for Ly-6C monocytes and a major determinant for Ly-6C cell recruitment to sites of atherosclerosis in mice.

Original languageEnglish (US)
Pages (from-to)3227-3237
Number of pages11
JournalCirculation
Volume117
Issue number25
DOIs
StatePublished - Jun 24 2008

Fingerprint

Monocytes
Atherosclerosis
Apolipoproteins E
E-Selectin
P-Selectin
Neointima
P-selectin ligand protein
Endothelium
Genes
L-Selectin
Atherosclerotic Plaques
Carotid Arteries
Leukocytes
Ligands

Keywords

  • Atherosclerosis
  • Cell adhesion molecules
  • Endothelium
  • Leukocytes

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

P-selectin glycoprotein ligand-1 is highly expressed on ly-6Chi monocytes and a major determinant for ly-6Chi monocyte recruitment to sites of atherosclerosis in mice. / An, Guangyu; Wang, Huan; Tang, Rong; Yago, Tadayuki; McDaniel, J. Michael; McGee, Samuel; Huo, Yuqing; Xia, Lijun.

In: Circulation, Vol. 117, No. 25, 24.06.2008, p. 3227-3237.

Research output: Contribution to journalArticle

An, Guangyu ; Wang, Huan ; Tang, Rong ; Yago, Tadayuki ; McDaniel, J. Michael ; McGee, Samuel ; Huo, Yuqing ; Xia, Lijun. / P-selectin glycoprotein ligand-1 is highly expressed on ly-6Chi monocytes and a major determinant for ly-6Chi monocyte recruitment to sites of atherosclerosis in mice. In: Circulation. 2008 ; Vol. 117, No. 25. pp. 3227-3237.
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abstract = "Background - Ly-6C monocytes are key contributors to atherosclerosis in mice. However, the manner in which Ly-6C monocytes selectively accumulate in atherosclerotic lesions is largely unknown. Monocyte homing to sites of atherosclerosis is primarily initiated by rolling on P- and E-selectin expressed on endothelium. We hypothesize that P-selectin glycoprotein ligand-1 (PSGL-1), the common ligand of P- and E-selectin on leukocytes, contributes to the preferential homing of Ly-6C monocytes to atherosclerotic lesions. Methods and Results - To test this hypothesis, we examined the expression and function of PSGL-1 on Ly-6C and Ly-6C monocytes from wild-type mice, ApoE mice, and mice lacking both ApoE and PSGL-1 genes (ApoE/PSGL-1). We found that Ly-6C monocytes expressed a higher level of PSGL-1 and had enhanced binding to fluid-phase P- and E-selectin compared with Ly-6C monocytes. Under in vitro flow conditions, more Ly-6C monocytes rolled on P-, E-, and L-selectin at slower velocities than Ly-6C cells. In an ex vivo perfused carotid artery model, Ly-6C monocytes interacted preferentially with atherosclerotic endothelium compared with Ly-6C monocytes in a PSGL-1-dependent manner. In vivo, ApoE mice lacking PSGL-1 had impaired Ly-6C monocyte recruitment to atherosclerotic lesions. Moreover, ApoE/PSGL-1 mice exhibited significantly reduced monocyte infiltration in wire injury-induced neointima and in atherosclerotic lesions. ApoE/PSGL-1 mice also developed smaller neointima and atherosclerotic plaques. Conclusions - These data indicate that PSGL-1 is a new marker for Ly-6C monocytes and a major determinant for Ly-6C cell recruitment to sites of atherosclerosis in mice.",
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T1 - P-selectin glycoprotein ligand-1 is highly expressed on ly-6Chi monocytes and a major determinant for ly-6Chi monocyte recruitment to sites of atherosclerosis in mice

AU - An, Guangyu

AU - Wang, Huan

AU - Tang, Rong

AU - Yago, Tadayuki

AU - McDaniel, J. Michael

AU - McGee, Samuel

AU - Huo, Yuqing

AU - Xia, Lijun

PY - 2008/6/24

Y1 - 2008/6/24

N2 - Background - Ly-6C monocytes are key contributors to atherosclerosis in mice. However, the manner in which Ly-6C monocytes selectively accumulate in atherosclerotic lesions is largely unknown. Monocyte homing to sites of atherosclerosis is primarily initiated by rolling on P- and E-selectin expressed on endothelium. We hypothesize that P-selectin glycoprotein ligand-1 (PSGL-1), the common ligand of P- and E-selectin on leukocytes, contributes to the preferential homing of Ly-6C monocytes to atherosclerotic lesions. Methods and Results - To test this hypothesis, we examined the expression and function of PSGL-1 on Ly-6C and Ly-6C monocytes from wild-type mice, ApoE mice, and mice lacking both ApoE and PSGL-1 genes (ApoE/PSGL-1). We found that Ly-6C monocytes expressed a higher level of PSGL-1 and had enhanced binding to fluid-phase P- and E-selectin compared with Ly-6C monocytes. Under in vitro flow conditions, more Ly-6C monocytes rolled on P-, E-, and L-selectin at slower velocities than Ly-6C cells. In an ex vivo perfused carotid artery model, Ly-6C monocytes interacted preferentially with atherosclerotic endothelium compared with Ly-6C monocytes in a PSGL-1-dependent manner. In vivo, ApoE mice lacking PSGL-1 had impaired Ly-6C monocyte recruitment to atherosclerotic lesions. Moreover, ApoE/PSGL-1 mice exhibited significantly reduced monocyte infiltration in wire injury-induced neointima and in atherosclerotic lesions. ApoE/PSGL-1 mice also developed smaller neointima and atherosclerotic plaques. Conclusions - These data indicate that PSGL-1 is a new marker for Ly-6C monocytes and a major determinant for Ly-6C cell recruitment to sites of atherosclerosis in mice.

AB - Background - Ly-6C monocytes are key contributors to atherosclerosis in mice. However, the manner in which Ly-6C monocytes selectively accumulate in atherosclerotic lesions is largely unknown. Monocyte homing to sites of atherosclerosis is primarily initiated by rolling on P- and E-selectin expressed on endothelium. We hypothesize that P-selectin glycoprotein ligand-1 (PSGL-1), the common ligand of P- and E-selectin on leukocytes, contributes to the preferential homing of Ly-6C monocytes to atherosclerotic lesions. Methods and Results - To test this hypothesis, we examined the expression and function of PSGL-1 on Ly-6C and Ly-6C monocytes from wild-type mice, ApoE mice, and mice lacking both ApoE and PSGL-1 genes (ApoE/PSGL-1). We found that Ly-6C monocytes expressed a higher level of PSGL-1 and had enhanced binding to fluid-phase P- and E-selectin compared with Ly-6C monocytes. Under in vitro flow conditions, more Ly-6C monocytes rolled on P-, E-, and L-selectin at slower velocities than Ly-6C cells. In an ex vivo perfused carotid artery model, Ly-6C monocytes interacted preferentially with atherosclerotic endothelium compared with Ly-6C monocytes in a PSGL-1-dependent manner. In vivo, ApoE mice lacking PSGL-1 had impaired Ly-6C monocyte recruitment to atherosclerotic lesions. Moreover, ApoE/PSGL-1 mice exhibited significantly reduced monocyte infiltration in wire injury-induced neointima and in atherosclerotic lesions. ApoE/PSGL-1 mice also developed smaller neointima and atherosclerotic plaques. Conclusions - These data indicate that PSGL-1 is a new marker for Ly-6C monocytes and a major determinant for Ly-6C cell recruitment to sites of atherosclerosis in mice.

KW - Atherosclerosis

KW - Cell adhesion molecules

KW - Endothelium

KW - Leukocytes

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