P-selectin inhibition suppresses muscle regeneration following injury

Wallace Baker, Barbara A. St. Pierre Schneider, Anhurunda Kulkarni, Gloria Sloan, Robert Schaub, Joseph Sypek, Joseph Gerard Cannon

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

This investigation sought to determine if P-selectin-mediated mechanisms contributed to macrophage localization in damaged muscle, an essential process for muscle regeneration. Mice were injected intravenously (i.v.) with soluble P-selectin glycoprotein ligand-1 (sPSGL-1) at 5, 50, or 200 μg/mouse or with 100 μl vehicle alone, and then, lengthening contractions were induced in hindlimb plantar-flexor muscles. The contractions caused fiber damage in soleus muscles, with maximal invasion by CD11b+ mononuclear cells at 24 h post-injury and substantial accumulation of CD11b+ mononuclear cells in the extracellular matrix up to 7 days post-injury. sPSGL-1 treatment caused a dose-dependent decrease in the number of regenerating fibers (P=0.021), as determined by developmental myosin heavy chain (dMHC) expression. This expression was reduced 93% at 7 days post-injury by the highest dose of sPSGL-1, which had no significant influence on intrafiber or extracellular accumulation of cells expressing CD11b, a general marker for phagocytic cells. Additional mice were injected i.v. with 20 μg anti-P-selectin or isotype-control immunoglobulin G and were then subjected to lengthening contractions as before. At 7 days post-injury, soleus muscles from anti-P-selectin-treated mice contained 48% fewer mononuclear cells that bound ER-BMDM1 (P=0.019), a marker for mature macrophages and dendritic cells, and 84% fewer fibers expressing dMHC (P = 0.006), compared with muscles from isotype-injected, control mice. The number of CD11b+ cells was not significantly different between groups. The results are consistent with the concept that P-selectin is involved in the recruitment, maturation, and/or activation of cells that are critical for muscle fiber regeneration.

Original languageEnglish (US)
Pages (from-to)352-358
Number of pages7
JournalJournal of Leukocyte Biology
Volume76
Issue number2
DOIs
StatePublished - Aug 1 2004

Fingerprint

P-Selectin
Regeneration
Muscles
Wounds and Injuries
Myosin Heavy Chains
Skeletal Muscle
Macrophages
Hindlimb
Phagocytes
Dendritic Cells
Muscle Cells
Extracellular Matrix
Cell Count
Immunoglobulin G
P-selectin ligand protein

Keywords

  • Dendritic cell
  • Macrophage
  • Myotube
  • Satellite cell

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

Cite this

Baker, W., St. Pierre Schneider, B. A., Kulkarni, A., Sloan, G., Schaub, R., Sypek, J., & Cannon, J. G. (2004). P-selectin inhibition suppresses muscle regeneration following injury. Journal of Leukocyte Biology, 76(2), 352-358. https://doi.org/10.1189/jlb.1102528

P-selectin inhibition suppresses muscle regeneration following injury. / Baker, Wallace; St. Pierre Schneider, Barbara A.; Kulkarni, Anhurunda; Sloan, Gloria; Schaub, Robert; Sypek, Joseph; Cannon, Joseph Gerard.

In: Journal of Leukocyte Biology, Vol. 76, No. 2, 01.08.2004, p. 352-358.

Research output: Contribution to journalArticle

Baker, W, St. Pierre Schneider, BA, Kulkarni, A, Sloan, G, Schaub, R, Sypek, J & Cannon, JG 2004, 'P-selectin inhibition suppresses muscle regeneration following injury', Journal of Leukocyte Biology, vol. 76, no. 2, pp. 352-358. https://doi.org/10.1189/jlb.1102528
Baker W, St. Pierre Schneider BA, Kulkarni A, Sloan G, Schaub R, Sypek J et al. P-selectin inhibition suppresses muscle regeneration following injury. Journal of Leukocyte Biology. 2004 Aug 1;76(2):352-358. https://doi.org/10.1189/jlb.1102528
Baker, Wallace ; St. Pierre Schneider, Barbara A. ; Kulkarni, Anhurunda ; Sloan, Gloria ; Schaub, Robert ; Sypek, Joseph ; Cannon, Joseph Gerard. / P-selectin inhibition suppresses muscle regeneration following injury. In: Journal of Leukocyte Biology. 2004 ; Vol. 76, No. 2. pp. 352-358.
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