Packed red cell transfusion does not compromise chromosome analysis in newborns

Anita S. Kulharya, Bonnie A. Salbert, Karen N. Norris, Lloyd Cook, Patricia J. Larrison, David B. Flannery

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Purpose: Critically ill neonates are frequently transfused with packed red cells. Some of these transfused neonates also need chromosome analysis. There is a long-standing tradition in pediatrics of not performing chromosome analysis after transfusion. We wished to determine whether transfusion with packed red cells affect the cytogenetic results in neonates. Method: The medical records of all neonates at the Medical College of Georgia who had had chromosome analysis between June 1995 and June 1998 were reviewed. Ten neonates had received transfusion prior to cytogenetic testing. Of these 10 infants, two had been transfused two or more times. Routine cytogenetic analysis of 20 metaphases at 550-band level had been performed on all 10 patients. Heteromorphic markers were compared in 10 randomly selected metaphases for any discrepancy. To determine whether there were theoretical reasons to delay chromosome analysis in transfused neonates, samples of irradiated, and/or filtered, and nonfiltered blood were obtained from the blood bank and analyzed for the presence of lymphocytes. Results: Prior transfusion did not affect karyotype results. A nonmosaic abnormal karyotype was found in 3 of the 10 patients. A fourth patient's karyotype was 45,X/47,XXX. This mosaicism was constitutive and consistent as demonstrated by a follow-up chromosome analysis. All other abnormal karyotypes were consistent with the dysmorphic phenotype. Randomly selected metaphases did not show any differences in the identifiable heteromorphic markers in all 10 patients. Although there was a 50% chance of patients receiving blood from a donor of opposite sex, there were no instances in which cells with a karyotype of the opposite sex were found in the patients' blood. The irradiated and filtered cultured donor blood samples did not show any metaphases. However, metaphases were seen in the cultures from nonfiltered and nonirradiated donor blood. Conclusions: Based on these results one does not need to delay karyotyping babies who have had blood transfusions. Packed red cell transfusion in newborns does not compromise the accuracy of chromosome analysis in our study even with multiple transfusions.

Original languageEnglish (US)
Pages (from-to)314-317
Number of pages4
JournalGenetics in Medicine
Volume3
Issue number4
DOIs
StatePublished - Jan 1 2001

Fingerprint

Chromosomes
Metaphase
Newborn Infant
Blood Donors
Karyotype
Abnormal Karyotype
Cytogenetics
Karyotyping
Blood Banks
Mosaicism
Cytogenetic Analysis
Critical Illness
Blood Transfusion
Medical Records
Lymphocytes
Pediatrics
Phenotype

Keywords

  • Cytogenetics
  • Dysmorphic
  • Karyotype
  • Mosaicism
  • Red blood cells
  • Transfusion

ASJC Scopus subject areas

  • Genetics(clinical)

Cite this

Kulharya, A. S., Salbert, B. A., Norris, K. N., Cook, L., Larrison, P. J., & Flannery, D. B. (2001). Packed red cell transfusion does not compromise chromosome analysis in newborns. Genetics in Medicine, 3(4), 314-317. https://doi.org/10.1097/00125817-200107000-00008

Packed red cell transfusion does not compromise chromosome analysis in newborns. / Kulharya, Anita S.; Salbert, Bonnie A.; Norris, Karen N.; Cook, Lloyd; Larrison, Patricia J.; Flannery, David B.

In: Genetics in Medicine, Vol. 3, No. 4, 01.01.2001, p. 314-317.

Research output: Contribution to journalArticle

Kulharya, AS, Salbert, BA, Norris, KN, Cook, L, Larrison, PJ & Flannery, DB 2001, 'Packed red cell transfusion does not compromise chromosome analysis in newborns', Genetics in Medicine, vol. 3, no. 4, pp. 314-317. https://doi.org/10.1097/00125817-200107000-00008
Kulharya AS, Salbert BA, Norris KN, Cook L, Larrison PJ, Flannery DB. Packed red cell transfusion does not compromise chromosome analysis in newborns. Genetics in Medicine. 2001 Jan 1;3(4):314-317. https://doi.org/10.1097/00125817-200107000-00008
Kulharya, Anita S. ; Salbert, Bonnie A. ; Norris, Karen N. ; Cook, Lloyd ; Larrison, Patricia J. ; Flannery, David B. / Packed red cell transfusion does not compromise chromosome analysis in newborns. In: Genetics in Medicine. 2001 ; Vol. 3, No. 4. pp. 314-317.
@article{5adb75e377854b7f8ee3697d6128e4d8,
title = "Packed red cell transfusion does not compromise chromosome analysis in newborns",
abstract = "Purpose: Critically ill neonates are frequently transfused with packed red cells. Some of these transfused neonates also need chromosome analysis. There is a long-standing tradition in pediatrics of not performing chromosome analysis after transfusion. We wished to determine whether transfusion with packed red cells affect the cytogenetic results in neonates. Method: The medical records of all neonates at the Medical College of Georgia who had had chromosome analysis between June 1995 and June 1998 were reviewed. Ten neonates had received transfusion prior to cytogenetic testing. Of these 10 infants, two had been transfused two or more times. Routine cytogenetic analysis of 20 metaphases at 550-band level had been performed on all 10 patients. Heteromorphic markers were compared in 10 randomly selected metaphases for any discrepancy. To determine whether there were theoretical reasons to delay chromosome analysis in transfused neonates, samples of irradiated, and/or filtered, and nonfiltered blood were obtained from the blood bank and analyzed for the presence of lymphocytes. Results: Prior transfusion did not affect karyotype results. A nonmosaic abnormal karyotype was found in 3 of the 10 patients. A fourth patient's karyotype was 45,X/47,XXX. This mosaicism was constitutive and consistent as demonstrated by a follow-up chromosome analysis. All other abnormal karyotypes were consistent with the dysmorphic phenotype. Randomly selected metaphases did not show any differences in the identifiable heteromorphic markers in all 10 patients. Although there was a 50{\%} chance of patients receiving blood from a donor of opposite sex, there were no instances in which cells with a karyotype of the opposite sex were found in the patients' blood. The irradiated and filtered cultured donor blood samples did not show any metaphases. However, metaphases were seen in the cultures from nonfiltered and nonirradiated donor blood. Conclusions: Based on these results one does not need to delay karyotyping babies who have had blood transfusions. Packed red cell transfusion in newborns does not compromise the accuracy of chromosome analysis in our study even with multiple transfusions.",
keywords = "Cytogenetics, Dysmorphic, Karyotype, Mosaicism, Red blood cells, Transfusion",
author = "Kulharya, {Anita S.} and Salbert, {Bonnie A.} and Norris, {Karen N.} and Lloyd Cook and Larrison, {Patricia J.} and Flannery, {David B.}",
year = "2001",
month = "1",
day = "1",
doi = "10.1097/00125817-200107000-00008",
language = "English (US)",
volume = "3",
pages = "314--317",
journal = "Genetics in Medicine",
issn = "1098-3600",
publisher = "Lippincott Williams and Wilkins",
number = "4",

}

TY - JOUR

T1 - Packed red cell transfusion does not compromise chromosome analysis in newborns

AU - Kulharya, Anita S.

AU - Salbert, Bonnie A.

AU - Norris, Karen N.

AU - Cook, Lloyd

AU - Larrison, Patricia J.

AU - Flannery, David B.

PY - 2001/1/1

Y1 - 2001/1/1

N2 - Purpose: Critically ill neonates are frequently transfused with packed red cells. Some of these transfused neonates also need chromosome analysis. There is a long-standing tradition in pediatrics of not performing chromosome analysis after transfusion. We wished to determine whether transfusion with packed red cells affect the cytogenetic results in neonates. Method: The medical records of all neonates at the Medical College of Georgia who had had chromosome analysis between June 1995 and June 1998 were reviewed. Ten neonates had received transfusion prior to cytogenetic testing. Of these 10 infants, two had been transfused two or more times. Routine cytogenetic analysis of 20 metaphases at 550-band level had been performed on all 10 patients. Heteromorphic markers were compared in 10 randomly selected metaphases for any discrepancy. To determine whether there were theoretical reasons to delay chromosome analysis in transfused neonates, samples of irradiated, and/or filtered, and nonfiltered blood were obtained from the blood bank and analyzed for the presence of lymphocytes. Results: Prior transfusion did not affect karyotype results. A nonmosaic abnormal karyotype was found in 3 of the 10 patients. A fourth patient's karyotype was 45,X/47,XXX. This mosaicism was constitutive and consistent as demonstrated by a follow-up chromosome analysis. All other abnormal karyotypes were consistent with the dysmorphic phenotype. Randomly selected metaphases did not show any differences in the identifiable heteromorphic markers in all 10 patients. Although there was a 50% chance of patients receiving blood from a donor of opposite sex, there were no instances in which cells with a karyotype of the opposite sex were found in the patients' blood. The irradiated and filtered cultured donor blood samples did not show any metaphases. However, metaphases were seen in the cultures from nonfiltered and nonirradiated donor blood. Conclusions: Based on these results one does not need to delay karyotyping babies who have had blood transfusions. Packed red cell transfusion in newborns does not compromise the accuracy of chromosome analysis in our study even with multiple transfusions.

AB - Purpose: Critically ill neonates are frequently transfused with packed red cells. Some of these transfused neonates also need chromosome analysis. There is a long-standing tradition in pediatrics of not performing chromosome analysis after transfusion. We wished to determine whether transfusion with packed red cells affect the cytogenetic results in neonates. Method: The medical records of all neonates at the Medical College of Georgia who had had chromosome analysis between June 1995 and June 1998 were reviewed. Ten neonates had received transfusion prior to cytogenetic testing. Of these 10 infants, two had been transfused two or more times. Routine cytogenetic analysis of 20 metaphases at 550-band level had been performed on all 10 patients. Heteromorphic markers were compared in 10 randomly selected metaphases for any discrepancy. To determine whether there were theoretical reasons to delay chromosome analysis in transfused neonates, samples of irradiated, and/or filtered, and nonfiltered blood were obtained from the blood bank and analyzed for the presence of lymphocytes. Results: Prior transfusion did not affect karyotype results. A nonmosaic abnormal karyotype was found in 3 of the 10 patients. A fourth patient's karyotype was 45,X/47,XXX. This mosaicism was constitutive and consistent as demonstrated by a follow-up chromosome analysis. All other abnormal karyotypes were consistent with the dysmorphic phenotype. Randomly selected metaphases did not show any differences in the identifiable heteromorphic markers in all 10 patients. Although there was a 50% chance of patients receiving blood from a donor of opposite sex, there were no instances in which cells with a karyotype of the opposite sex were found in the patients' blood. The irradiated and filtered cultured donor blood samples did not show any metaphases. However, metaphases were seen in the cultures from nonfiltered and nonirradiated donor blood. Conclusions: Based on these results one does not need to delay karyotyping babies who have had blood transfusions. Packed red cell transfusion in newborns does not compromise the accuracy of chromosome analysis in our study even with multiple transfusions.

KW - Cytogenetics

KW - Dysmorphic

KW - Karyotype

KW - Mosaicism

KW - Red blood cells

KW - Transfusion

UR - http://www.scopus.com/inward/record.url?scp=0035746696&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035746696&partnerID=8YFLogxK

U2 - 10.1097/00125817-200107000-00008

DO - 10.1097/00125817-200107000-00008

M3 - Article

C2 - 11478533

AN - SCOPUS:0035746696

VL - 3

SP - 314

EP - 317

JO - Genetics in Medicine

JF - Genetics in Medicine

SN - 1098-3600

IS - 4

ER -