Parathyroid hormone effects on signaling pathways in endothelial cells vary with peptide concentration

Doug Throckmorton, Doris Kurscheid-Reich, Oscar R. Rosales, Jose Rodriguez-Commes, Raquel Lopez, Bauer Sumpio, Qing Zhong, Kehong Ding, Richard McCarthy, Paula Q. Barrett, Carlos M Isales

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We have previously reported that parathyroid hormone (PTH) has specific effects on a human umbilical vein endothelial cell line. Further studies were performed to characterize the signaling cascades initiated by PTH. We report that PTH induced the appearance of voltage sensitive calcium channels. Furthermore, PTH increased ceramide but not diacylglycerol content. Since elevations in [Ca2+]i and phospholipid turnover are signals for the activation of protein kinase C (PKC), the cells were screened for PKC isoforms. PTH induced a redistribution of the PKCε to the particulate fractions of cell homogenates. In summary, PTH induced PKC translocation through a calcium-phospholipid pathway in an endothelial cell line.

Original languageEnglish (US)
Pages (from-to)79-85
Number of pages7
JournalPeptides
Volume23
Issue number1
DOIs
StatePublished - Feb 11 2002

Fingerprint

Endothelial cells
Parathyroid Hormone
Endothelial Cells
Protein Kinase C
Peptides
Phospholipids
Cell Line
Ceramides
Diglycerides
Human Umbilical Vein Endothelial Cells
Calcium Channels
Protein Isoforms
Chemical activation
Calcium
Electric potential

Keywords

  • Calcium channels
  • Ceramide
  • Diacylglycerol
  • PTH
  • Protein kinase C

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

Cite this

Throckmorton, D., Kurscheid-Reich, D., Rosales, O. R., Rodriguez-Commes, J., Lopez, R., Sumpio, B., ... Isales, C. M. (2002). Parathyroid hormone effects on signaling pathways in endothelial cells vary with peptide concentration. Peptides, 23(1), 79-85. https://doi.org/10.1016/S0196-9781(01)00582-4

Parathyroid hormone effects on signaling pathways in endothelial cells vary with peptide concentration. / Throckmorton, Doug; Kurscheid-Reich, Doris; Rosales, Oscar R.; Rodriguez-Commes, Jose; Lopez, Raquel; Sumpio, Bauer; Zhong, Qing; Ding, Kehong; McCarthy, Richard; Barrett, Paula Q.; Isales, Carlos M.

In: Peptides, Vol. 23, No. 1, 11.02.2002, p. 79-85.

Research output: Contribution to journalArticle

Throckmorton, D, Kurscheid-Reich, D, Rosales, OR, Rodriguez-Commes, J, Lopez, R, Sumpio, B, Zhong, Q, Ding, K, McCarthy, R, Barrett, PQ & Isales, CM 2002, 'Parathyroid hormone effects on signaling pathways in endothelial cells vary with peptide concentration', Peptides, vol. 23, no. 1, pp. 79-85. https://doi.org/10.1016/S0196-9781(01)00582-4
Throckmorton D, Kurscheid-Reich D, Rosales OR, Rodriguez-Commes J, Lopez R, Sumpio B et al. Parathyroid hormone effects on signaling pathways in endothelial cells vary with peptide concentration. Peptides. 2002 Feb 11;23(1):79-85. https://doi.org/10.1016/S0196-9781(01)00582-4
Throckmorton, Doug ; Kurscheid-Reich, Doris ; Rosales, Oscar R. ; Rodriguez-Commes, Jose ; Lopez, Raquel ; Sumpio, Bauer ; Zhong, Qing ; Ding, Kehong ; McCarthy, Richard ; Barrett, Paula Q. ; Isales, Carlos M. / Parathyroid hormone effects on signaling pathways in endothelial cells vary with peptide concentration. In: Peptides. 2002 ; Vol. 23, No. 1. pp. 79-85.
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