Parathyroid hormone effects on signaling pathways in endothelial cells vary with peptide concentration

Doug Throckmorton, Doris Kurscheid-Reich, Oscar R. Rosales, Jose Rodriguez-Commes, Raquel Lopez, Bauer Sumpio, Qing Zhong, Ke Hong Ding, Richard McCarthy, Paula Q. Barrett, Carlos M. Isales

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

We have previously reported that parathyroid hormone (PTH) has specific effects on a human umbilical vein endothelial cell line. Further studies were performed to characterize the signaling cascades initiated by PTH. We report that PTH induced the appearance of voltage sensitive calcium channels. Furthermore, PTH increased ceramide but not diacylglycerol content. Since elevations in [Ca2+]i and phospholipid turnover are signals for the activation of protein kinase C (PKC), the cells were screened for PKC isoforms. PTH induced a redistribution of the PKCε to the particulate fractions of cell homogenates. In summary, PTH induced PKC translocation through a calcium-phospholipid pathway in an endothelial cell line.

Original languageEnglish (US)
Pages (from-to)79-85
Number of pages7
JournalPeptides
Volume23
Issue number1
DOIs
StatePublished - Feb 11 2002

Keywords

  • Calcium channels
  • Ceramide
  • Diacylglycerol
  • PTH
  • Protein kinase C

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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    Throckmorton, D., Kurscheid-Reich, D., Rosales, O. R., Rodriguez-Commes, J., Lopez, R., Sumpio, B., Zhong, Q., Ding, K. H., McCarthy, R., Barrett, P. Q., & Isales, C. M. (2002). Parathyroid hormone effects on signaling pathways in endothelial cells vary with peptide concentration. Peptides, 23(1), 79-85. https://doi.org/10.1016/S0196-9781(01)00582-4