Parathyroid hormone modulates angiotensin II-induced aldosterone secretion from the adrenal glomerulosa cell

Carlos M Isales, Paula Q. Barrett, Michael Brines, Wendy B Bollag, Howard Rasmussen

Research output: Contribution to journalArticle

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Abstract

The effect of PTH on aldosterone secretion from isolated bovine adrenal glomerulosa cells was examined. PTH binding was autoradiographically localized to the adrenal cortex, suggesting a specific effect. This binding of PTH was displaceable by cold PTH, but not by ACTH. No binding was observed in the adrenal medulla. In addition, PTH was shown to stimulate aldosterone secretion in a dose-dependent manner and to potentiate aldosterone secretion in response to angioten-sin-II, such that PTH (10-9m) elevated the secretory rate from 58.6 ± 6.8 to 110.9 ± 19 pg/min-million cells in the presence of 10 nM angiotensin-II. The magnitude of the synergism between the two hormones depended on the concentrations of PTH and angiotensin-II as well as the time during which aldosterone secretion was measured. Within the first 15 min of stimulation, PTH increased the sensitivity to angiotensin-II, shifting the Kafor activation from 1.0 to 0.3 nM. In contrast, between 30-45 min of angiotensin-II stimulation, PTH elevated the maximal secretory response to angiotensin-II from 109 ± 3.4 to 219 ± 13.3 pg/min · million cells. By itself PTH elicited only a small increase in the intracellular Ca2+concentration, as measured by aequorin luminescence in glomerulosa cells. In cells pretreated with angiotensin-II or 15 mM potassium, the intracellular calcium response to PTH was markedly potentiated. PTH was also found to cause a small increase in the cellular cAMP content. Thus, PTH stimulates aldosterone secretion from adrenal glomerulosa cells, both alone and in combination with angiotensin-II.

Original languageEnglish (US)
Pages (from-to)489-495
Number of pages7
JournalEndocrinology
Volume129
Issue number1
DOIs
StatePublished - Jan 1 1991
Externally publishedYes

Fingerprint

Zona Glomerulosa
Aldosterone
Parathyroid Hormone
Angiotensin II
Secretory Rate
Aequorin
Adrenal Medulla
Adrenal Cortex
Luminescence
Adrenocorticotropic Hormone
Potassium
Hormones
Calcium

ASJC Scopus subject areas

  • Endocrinology

Cite this

Parathyroid hormone modulates angiotensin II-induced aldosterone secretion from the adrenal glomerulosa cell. / Isales, Carlos M; Barrett, Paula Q.; Brines, Michael; Bollag, Wendy B; Rasmussen, Howard.

In: Endocrinology, Vol. 129, No. 1, 01.01.1991, p. 489-495.

Research output: Contribution to journalArticle

Isales, Carlos M ; Barrett, Paula Q. ; Brines, Michael ; Bollag, Wendy B ; Rasmussen, Howard. / Parathyroid hormone modulates angiotensin II-induced aldosterone secretion from the adrenal glomerulosa cell. In: Endocrinology. 1991 ; Vol. 129, No. 1. pp. 489-495.
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