Partial amino-terminal sequences of the polyoma nonhistone proteins VP1, VP2, and VP3 synthesized in vitro

R. M. Hewick, A. Mellor, A. E. Smith, M. D. Waterfield

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The three polyoma virus capsid proteins VP1, VP2, and VP3 were synthesized in vitro in the presence of several radiolabeled amino acids and, after purification on sodium dodecyl sulfate-polyacrylamide gels, were subjected to sequential Edman degradation. The partial amino-terminal amino acid sequences obtained were compared with the sequence of amino acids predicted from the polyoma virus DNA sequencing. Together, these results showed that the 5' ends of the VP1, VP2, and VP3 coding sequences are located 1,217, 289, and 634 nucleotides, respectively, from the junction of HpaII restriction fragments 3 and 5.

Original languageEnglish (US)
Pages (from-to)631-636
Number of pages6
JournalJournal of Virology
Volume33
Issue number2
StatePublished - Jan 1 1980

Fingerprint

Polyomavirus
Amino Acid Sequence
amino acid sequences
viruses
Capsid Proteins
sodium dodecyl sulfate
polyacrylamide
coat proteins
DNA Sequence Analysis
Sodium Dodecyl Sulfate
Proteins
Nucleotides
sequence analysis
proteins
nucleotides
gels
Amino Acids
amino acids
degradation
In Vitro Techniques

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Hewick, R. M., Mellor, A., Smith, A. E., & Waterfield, M. D. (1980). Partial amino-terminal sequences of the polyoma nonhistone proteins VP1, VP2, and VP3 synthesized in vitro. Journal of Virology, 33(2), 631-636.

Partial amino-terminal sequences of the polyoma nonhistone proteins VP1, VP2, and VP3 synthesized in vitro. / Hewick, R. M.; Mellor, A.; Smith, A. E.; Waterfield, M. D.

In: Journal of Virology, Vol. 33, No. 2, 01.01.1980, p. 631-636.

Research output: Contribution to journalArticle

Hewick, RM, Mellor, A, Smith, AE & Waterfield, MD 1980, 'Partial amino-terminal sequences of the polyoma nonhistone proteins VP1, VP2, and VP3 synthesized in vitro', Journal of Virology, vol. 33, no. 2, pp. 631-636.
Hewick, R. M. ; Mellor, A. ; Smith, A. E. ; Waterfield, M. D. / Partial amino-terminal sequences of the polyoma nonhistone proteins VP1, VP2, and VP3 synthesized in vitro. In: Journal of Virology. 1980 ; Vol. 33, No. 2. pp. 631-636.
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