Patching plasma membrane disruptions with cytoplasmic membrane

Paul L. McNeil, Steven S. Vogel, Katsuya Miyake, Mark Terasaki

Research output: Contribution to journalArticle

132 Citations (Scopus)

Abstract

Vesicle-vesicle fusion initiated in cell cytoplasm by high Ca2+ can rapidly erect large membrane boundaries. These might be used as a 'patch' for resealing plasma membrane disruptions. Three central predictions of this 'patch' hypothesis are here established in sea urchin eggs. First, we show that surface markers for plasma membrane protein and lipid are initially absent over disruption sites after resealing is complete. Second, we demonstrate that resealing capacity is strongly dependent upon local availability of fusion competent cytoplasmic organelles, specifically the reserve or yolk granule. Lastly, we demonstrate that the reserve granule is capable of rapid (t 1/2 < 1 second), Ca2+-regulated thigh threshold) fusion capable of erecting large (> 1000 μm2), continuous membrane boundaries. Production of patch vesicles for resealing may proceed by an 'emergency' fusion mechanism distinct from that utilized for the much slower, highly regulated, cytosol-requiring organelle-organelle fusion events typical of constitutive membrane trafficking pathways.

Original languageEnglish (US)
Pages (from-to)1891-1902
Number of pages12
JournalJournal of Cell Science
Volume113
Issue number11
StatePublished - Jun 28 2000

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Organelles
Cell Membrane
Membranes
Sea Urchins
Membrane Lipids
Cytosol
Eggs
Blood Proteins
Membrane Proteins
Cytoplasm
Emergencies

Keywords

  • Disruption
  • Fusion
  • Homotypic
  • Plasma membrane
  • Resealing

ASJC Scopus subject areas

  • Cell Biology

Cite this

McNeil, P. L., Vogel, S. S., Miyake, K., & Terasaki, M. (2000). Patching plasma membrane disruptions with cytoplasmic membrane. Journal of Cell Science, 113(11), 1891-1902.

Patching plasma membrane disruptions with cytoplasmic membrane. / McNeil, Paul L.; Vogel, Steven S.; Miyake, Katsuya; Terasaki, Mark.

In: Journal of Cell Science, Vol. 113, No. 11, 28.06.2000, p. 1891-1902.

Research output: Contribution to journalArticle

McNeil, PL, Vogel, SS, Miyake, K & Terasaki, M 2000, 'Patching plasma membrane disruptions with cytoplasmic membrane', Journal of Cell Science, vol. 113, no. 11, pp. 1891-1902.
McNeil PL, Vogel SS, Miyake K, Terasaki M. Patching plasma membrane disruptions with cytoplasmic membrane. Journal of Cell Science. 2000 Jun 28;113(11):1891-1902.
McNeil, Paul L. ; Vogel, Steven S. ; Miyake, Katsuya ; Terasaki, Mark. / Patching plasma membrane disruptions with cytoplasmic membrane. In: Journal of Cell Science. 2000 ; Vol. 113, No. 11. pp. 1891-1902.
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