Abstract
Objectives: To describe the strengths and limitations of the available influenza diagnostics, with a focus on rapid antigen detection assays and nucleic acid detection assays. Methods: A case-based presentation is used to illustrate the potential limitations of rapid antigen detection assays for influenza. Results: Influenza is a seasonal illness; estimates attribute influenza to approximately 200,000 hospitalizations and 41,000 deaths in the United States annually. Antigen detection assays for influenza are rapid and convenient, and thus are widely used in a variety of health care settings, even though the sensitivity of these assays may be suboptimal. The United States Food and Drug Administration has recently created new guidelines intended to improve the oversight and performance characteristics of influenza antigen detection assays. Molecular assays, although more costly and complex, are more sensitive and may be designed to simultaneously detect multiple respiratory pathogens within a single assay. Conclusions: Diagnostic assays for influenza can vary greatly with regards to analytical performance characteristics, complexity, turnaround time and cost. This can have important patient care and infection prevention implications.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 440-448 |
| Number of pages | 9 |
| Journal | American Journal of Clinical Pathology |
| Volume | 145 |
| Issue number | 4 |
| DOIs | |
| State | Published - Apr 1 2016 |
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Keywords
- Diagnostics
- Influenza
- Nucleic acid amplification tests
- Rapid influenza diagnostic test
- Respiratory virus
ASJC Scopus subject areas
- Pathology and Forensic Medicine
Cite this
Pathology consultation on influenza diagnostics. / McMullen, Allison R.; Anderson, Neil W.; Burnhamfor, Carey Ann D.
In: American Journal of Clinical Pathology, Vol. 145, No. 4, 01.04.2016, p. 440-448.Research output: Contribution to journal › Review article
}
TY - JOUR
T1 - Pathology consultation on influenza diagnostics
AU - McMullen, Allison R.
AU - Anderson, Neil W.
AU - Burnhamfor, Carey Ann D.
PY - 2016/4/1
Y1 - 2016/4/1
N2 - Objectives: To describe the strengths and limitations of the available influenza diagnostics, with a focus on rapid antigen detection assays and nucleic acid detection assays. Methods: A case-based presentation is used to illustrate the potential limitations of rapid antigen detection assays for influenza. Results: Influenza is a seasonal illness; estimates attribute influenza to approximately 200,000 hospitalizations and 41,000 deaths in the United States annually. Antigen detection assays for influenza are rapid and convenient, and thus are widely used in a variety of health care settings, even though the sensitivity of these assays may be suboptimal. The United States Food and Drug Administration has recently created new guidelines intended to improve the oversight and performance characteristics of influenza antigen detection assays. Molecular assays, although more costly and complex, are more sensitive and may be designed to simultaneously detect multiple respiratory pathogens within a single assay. Conclusions: Diagnostic assays for influenza can vary greatly with regards to analytical performance characteristics, complexity, turnaround time and cost. This can have important patient care and infection prevention implications.
AB - Objectives: To describe the strengths and limitations of the available influenza diagnostics, with a focus on rapid antigen detection assays and nucleic acid detection assays. Methods: A case-based presentation is used to illustrate the potential limitations of rapid antigen detection assays for influenza. Results: Influenza is a seasonal illness; estimates attribute influenza to approximately 200,000 hospitalizations and 41,000 deaths in the United States annually. Antigen detection assays for influenza are rapid and convenient, and thus are widely used in a variety of health care settings, even though the sensitivity of these assays may be suboptimal. The United States Food and Drug Administration has recently created new guidelines intended to improve the oversight and performance characteristics of influenza antigen detection assays. Molecular assays, although more costly and complex, are more sensitive and may be designed to simultaneously detect multiple respiratory pathogens within a single assay. Conclusions: Diagnostic assays for influenza can vary greatly with regards to analytical performance characteristics, complexity, turnaround time and cost. This can have important patient care and infection prevention implications.
KW - Diagnostics
KW - Influenza
KW - Nucleic acid amplification tests
KW - Rapid influenza diagnostic test
KW - Respiratory virus
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UR - http://www.scopus.com/inward/citedby.url?scp=84981251925&partnerID=8YFLogxK
U2 - 10.1093/AJCP/AQW039
DO - 10.1093/AJCP/AQW039
M3 - Review article
C2 - 27124947
AN - SCOPUS:84981251925
VL - 145
SP - 440
EP - 448
JO - American Journal of Clinical Pathology
JF - American Journal of Clinical Pathology
SN - 0002-9173
IS - 4
ER -