Patterns of nephrin and a new proteinuria-associated protein expression in human renal diseases

Shixuan Wang, Maria P. Rastaldi, Anu Pätäri, Heikki Ahola, Eija Heikkilä, Harry Holthöfer

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Background. Many factors contribute to the pathogenesis of glomerular proteinuria, but no exact molecular mechanisms are known to date. The recently reported protein nephrin, encoded by the NPHS1 gene, appears to be crucial for the integrity of the glomerular filtration barrier. Methods. Immunohistochemistry was used to detect possible changes in glomerular nephrin, and a new proteinuria-associated protein expression was developed in various diagnostic groups of human kidney biopsies. Results. In normal control kidney, antibodies to intracellular and extracellular nephrin domain showed a typical podocyte pattern of reactivity, while the 18C7 antibody to a normally inaccessible proteinuria-associated epitope was negative. Instead, strong glomerular positivity by 18C7 was seen in membranous glomerulonephropathy, membranoproliferative glomerulonephritis, systemic lupus erythematosus and cryoglobulinemic nephritis, while with antibodies to either intracellular or extracellular nephrin domains, a down-regulation in nephrin expression pattern was shown. Conclusions. Unmasking or de novo expression of distinct glomerular proteins may be an important feature reflecting the pathophysiological events in these diseases with altered glomerular permeability, while only mild changes in the slit diaphragm protein nephrin appear to take place.

Original languageEnglish (US)
Pages (from-to)141-147
Number of pages7
JournalKidney International
Volume61
Issue number1
DOIs
StatePublished - Jan 1 2002

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Proteinuria
Kidney
Proteins
Antibodies
Glomerular Filtration Barrier
Membranoproliferative Glomerulonephritis
Podocytes
Membranous Glomerulonephritis
Nephritis
Diaphragm
Systemic Lupus Erythematosus
nephrin
Epitopes
Permeability
Down-Regulation
Immunohistochemistry
Biopsy
Genes

Keywords

  • Filtration barrier
  • Glomerular basement membrane
  • Podocytes
  • Slit diaphragm
  • Transmembrane protein

ASJC Scopus subject areas

  • Nephrology

Cite this

Patterns of nephrin and a new proteinuria-associated protein expression in human renal diseases. / Wang, Shixuan; Rastaldi, Maria P.; Pätäri, Anu; Ahola, Heikki; Heikkilä, Eija; Holthöfer, Harry.

In: Kidney International, Vol. 61, No. 1, 01.01.2002, p. 141-147.

Research output: Contribution to journalArticle

Wang, Shixuan ; Rastaldi, Maria P. ; Pätäri, Anu ; Ahola, Heikki ; Heikkilä, Eija ; Holthöfer, Harry. / Patterns of nephrin and a new proteinuria-associated protein expression in human renal diseases. In: Kidney International. 2002 ; Vol. 61, No. 1. pp. 141-147.
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