Paxillin binding is not the sole determinant of focal adhesion localization or dominant-negative activity of focal adhesion kinase/focal adhesion kinase-related nonkinase

M. A. Cooley, J. M. Broome, C. Ohngemach, L. H. Romer, M. D. Schaller

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Abstract

The carboxy-terminal 150 residues of the focal adhesion kinase (FAK) comprise the focal adhesion-targeting sequence, which is responsible for its subcellular localization. The mechanism of focal adhesion targeting has not been fully elucidated. We describe a mutational analysis of the focal adhesion-targeting sequence of FAK to further examine the mechanism of focal adhesion targeting and explore additional functions encoded by the carboxy-terminus of FAK. The results demonstrate that paxillin binding is dispensable for focal adhesion targeting of FAK. Cell adhesion-dependent tyrosine phosphorylation strictly correlated with the ability of mutants to target to focal adhesions. Focal adhesion targeting was also a requirement for maximal FAK-dependent tyrosine phosphorylation of paxillin and FAK-related nonkinase (FRNK)-dependent inhibition of endogenous FAK function. However, there were additional requirements for these latter functions because we identified mutants that target to focal adhesions, yet are defective for the induction of paxillin phosphorylation or the dominant-negative function of FRNK. Furthermore, the paxillin-binding activity of FRNK mutants did not correlate with their ability to inhibit FAK, suggesting that FRNK has other targets in addition to paxillin.

Original languageEnglish (US)
Pages (from-to)3247-3263
Number of pages17
JournalMolecular Biology of the Cell
Volume11
Issue number9
DOIs
StatePublished - Jan 1 2000

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Paxillin
Focal Adhesion Protein-Tyrosine Kinases
Focal Adhesions
Phosphorylation
Tyrosine
Cell Adhesion

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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Paxillin binding is not the sole determinant of focal adhesion localization or dominant-negative activity of focal adhesion kinase/focal adhesion kinase-related nonkinase. / Cooley, M. A.; Broome, J. M.; Ohngemach, C.; Romer, L. H.; Schaller, M. D.

In: Molecular Biology of the Cell, Vol. 11, No. 9, 01.01.2000, p. 3247-3263.

Research output: Contribution to journalArticle

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