PCR-RFLP based genotyping of cattle using DNA extracted from hair samples

Pushpendra Kumar, Vivek Choudhary, T. K. Bhattacharya, B. Bhushan, Arjava Sharma

Research output: Contribution to journalArticle

Abstract

A simple method of genotyping of farm animals using DNA extracted from hair samples is described. Hair samples of 35 F x H (Holstein Friesian x Hariana) crossbred cattle were processed for isolation of genomic DNA. These DNA samples were used for genotyping of insulin-like growth factor binding protein 3 (IGFBP 3) gene using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Thus, hair samples could be an easy and reliable source of DNA for genotyping of farm animals or any other molecular genetic research work where DNA is needed as an experimental material for the study.

Original languageEnglish (US)
Pages (from-to)287-289
Number of pages3
JournalIndian Journal of Biotechnology
Volume4
Issue number2
StatePublished - Apr 1 2005

Keywords

  • DNA
  • FLP
  • Genotyping
  • Hair
  • PCR

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Plant Science

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    Kumar, P., Choudhary, V., Bhattacharya, T. K., Bhushan, B., & Sharma, A. (2005). PCR-RFLP based genotyping of cattle using DNA extracted from hair samples. Indian Journal of Biotechnology, 4(2), 287-289.