Peptide dependency of alloreactive CD4+ T cell responses

Sanjeev K. Mendiratta, Jean Paul Kovalik, Seokmann Hong, Nagendra Singh, W. David Martin, Luc Van Kaer

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Alloreactivity, the capacity of a large number of T lymphocytes to react with foreign MHC molecules, represents the cellular basis for the rejection of tissue grafts. Although it was originally assumed that the TCR of alloreactive T cells focus their recognition on the polymorphic residues that differ between the MHC molecules of responder and stimulator cells, studies in the MHC class I system have clearly demonstrated that MHC-bound peptides can influence this interaction. It remains unclear, however, whether peptides play an equally important role for the recognition of MHC class II molecules by alloreactive CD4+ T cells. Another issue that remains unresolved is the overall frequency of peptide-dependent versus peptide-independent alloreactive T cells. We have addressed these questions with antigen-presenting cells (APC) from H2-M mutant mice that predominantly express a single MHC class II-peptide complex, H2-Ab bound by a peptide (CLIP) derived from the class II-associated invariant chain. APC from these mice were used as targets and stimulators for alloreactive CD4+ T cells. Results demonstrated that the vast majority of CD4+ alloreactive T cells recognize MHC class II molecules in a peptide-dependent fashion.

Original languageEnglish (US)
Pages (from-to)351-360
Number of pages10
JournalInternational Immunology
Volume11
Issue number3
DOIs
StatePublished - Jan 1 1999
Externally publishedYes

Fingerprint

T-Lymphocytes
Peptides
Antigen-Presenting Cells
CD4 Antigens
Transplants
invariant chain

Keywords

  • Alloreactivity
  • Gene-targeted mouse
  • H2-M
  • MHC class II molecules
  • Peptides

ASJC Scopus subject areas

  • Immunology

Cite this

Mendiratta, S. K., Kovalik, J. P., Hong, S., Singh, N., Martin, W. D., & Van Kaer, L. (1999). Peptide dependency of alloreactive CD4+ T cell responses. International Immunology, 11(3), 351-360. https://doi.org/10.1093/intimm/11.3.351

Peptide dependency of alloreactive CD4+ T cell responses. / Mendiratta, Sanjeev K.; Kovalik, Jean Paul; Hong, Seokmann; Singh, Nagendra; Martin, W. David; Van Kaer, Luc.

In: International Immunology, Vol. 11, No. 3, 01.01.1999, p. 351-360.

Research output: Contribution to journalArticle

Mendiratta, SK, Kovalik, JP, Hong, S, Singh, N, Martin, WD & Van Kaer, L 1999, 'Peptide dependency of alloreactive CD4+ T cell responses', International Immunology, vol. 11, no. 3, pp. 351-360. https://doi.org/10.1093/intimm/11.3.351
Mendiratta SK, Kovalik JP, Hong S, Singh N, Martin WD, Van Kaer L. Peptide dependency of alloreactive CD4+ T cell responses. International Immunology. 1999 Jan 1;11(3):351-360. https://doi.org/10.1093/intimm/11.3.351
Mendiratta, Sanjeev K. ; Kovalik, Jean Paul ; Hong, Seokmann ; Singh, Nagendra ; Martin, W. David ; Van Kaer, Luc. / Peptide dependency of alloreactive CD4+ T cell responses. In: International Immunology. 1999 ; Vol. 11, No. 3. pp. 351-360.
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