Abstract
Alloreactivity, the capacity of a large number of T lymphocytes to react with foreign MHC molecules, represents the cellular basis for the rejection of tissue grafts. Although it was originally assumed that the TCR of alloreactive T cells focus their recognition on the polymorphic residues that differ between the MHC molecules of responder and stimulator cells, studies in the MHC class I system have clearly demonstrated that MHC-bound peptides can influence this interaction. It remains unclear, however, whether peptides play an equally important role for the recognition of MHC class II molecules by alloreactive CD4+ T cells. Another issue that remains unresolved is the overall frequency of peptide-dependent versus peptide-independent alloreactive T cells. We have addressed these questions with antigen-presenting cells (APC) from H2-M mutant mice that predominantly express a single MHC class II-peptide complex, H2-Ab bound by a peptide (CLIP) derived from the class II-associated invariant chain. APC from these mice were used as targets and stimulators for alloreactive CD4+ T cells. Results demonstrated that the vast majority of CD4+ alloreactive T cells recognize MHC class II molecules in a peptide-dependent fashion.
Original language | English (US) |
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Pages (from-to) | 351-360 |
Number of pages | 10 |
Journal | International Immunology |
Volume | 11 |
Issue number | 3 |
DOIs | |
State | Published - 1999 |
Externally published | Yes |
Keywords
- Alloreactivity
- Gene-targeted mouse
- H2-M
- MHC class II molecules
- Peptides
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology