Abstract
The cortex is a key brain region vulnerable to intracerebral hemorrhage (ICH) associated with stroke and head trauma. Animal models of ICH, via blood or collagenase infusion, have been developed most commonly to target the striatum. Here, we show that stereotaxic injection of collagenase type IV into two sites of the right cortex of adult C57BL6 mice produced hemorrhage to the cortex, subcortical white matter and hippocampus at day 1 post-injury, followed by cortical volume decrement by day 7. Reductions in MAP2- and NeuN-positive neurons were detected at day 1 and 7 post-injury in the core and peri-hemorrhagic cortex, respectively. Fluoro-Jade positive degenerating neurons were observed at day 1 in the peri-hemorrhagic area. An aberrant aggregation of GFAP-positive astrocytes and a significant reduction in RIP-positive oligodendroglial cells were detected at day 7 post-injury in the cortical area. In addition, a significant decrement in retrogradely Cholera Toxin Subunit B-labeled corticospinal neurons was recognized at day 14 post-injury in the ipsilateral cortex. Among the behavioral tests employed, the pole climb movement test robustly detected significant motor dysfunction at day 1, 3, and 7 post-injury that positively but inversely correlated with cortical volume at day 1 and 7 post-injury, respectively. The consistent observation of neuronal cell loss in the hemorrhagic core that subsequently extended to degeneration of neurons in the peri-hemorrhagic area, with accompanying motor abnormalities at least up to the subacute phase, advances this cortical hemorrhage model as a platform for examining the pathophysiology of and experimental treatments for ICH.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 228-239 |
| Number of pages | 12 |
| Journal | Brain Research |
| Volume | 1355 |
| DOIs | |
| State | Published - Oct 8 2010 |
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Keywords
- Behavior
- Head trauma
- Intracerebral hemorrhage
- Motor cortex
- Neurodegeneration
ASJC Scopus subject areas
- Neuroscience(all)
- Molecular Biology
- Clinical Neurology
- Developmental Biology
Cite this
Peri-hemorrhagic degeneration accompanies stereotaxic collagenase-mediated cortical hemorrhage in mouse. / Masuda, Tadashi; Maki, Mina; Hara, Koichi; Yasuhara, Takao; Matsukawa, Noriyuki; Yu, Seongjin; Bae, Eunkyung Cate; Tajiri, Naoki; Chheda, Sonia H.; Solomita, Marianna Aurora; Weinbren, Nathan; Kaneko, Yuji; Kirov, Sergei A.; Hess, David C.; Hida, Hideki; Borlongan, Cesar V.
In: Brain Research, Vol. 1355, 08.10.2010, p. 228-239.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Peri-hemorrhagic degeneration accompanies stereotaxic collagenase-mediated cortical hemorrhage in mouse
AU - Masuda, Tadashi
AU - Maki, Mina
AU - Hara, Koichi
AU - Yasuhara, Takao
AU - Matsukawa, Noriyuki
AU - Yu, Seongjin
AU - Bae, Eunkyung Cate
AU - Tajiri, Naoki
AU - Chheda, Sonia H.
AU - Solomita, Marianna Aurora
AU - Weinbren, Nathan
AU - Kaneko, Yuji
AU - Kirov, Sergei A.
AU - Hess, David C.
AU - Hida, Hideki
AU - Borlongan, Cesar V.
PY - 2010/10/8
Y1 - 2010/10/8
N2 - The cortex is a key brain region vulnerable to intracerebral hemorrhage (ICH) associated with stroke and head trauma. Animal models of ICH, via blood or collagenase infusion, have been developed most commonly to target the striatum. Here, we show that stereotaxic injection of collagenase type IV into two sites of the right cortex of adult C57BL6 mice produced hemorrhage to the cortex, subcortical white matter and hippocampus at day 1 post-injury, followed by cortical volume decrement by day 7. Reductions in MAP2- and NeuN-positive neurons were detected at day 1 and 7 post-injury in the core and peri-hemorrhagic cortex, respectively. Fluoro-Jade positive degenerating neurons were observed at day 1 in the peri-hemorrhagic area. An aberrant aggregation of GFAP-positive astrocytes and a significant reduction in RIP-positive oligodendroglial cells were detected at day 7 post-injury in the cortical area. In addition, a significant decrement in retrogradely Cholera Toxin Subunit B-labeled corticospinal neurons was recognized at day 14 post-injury in the ipsilateral cortex. Among the behavioral tests employed, the pole climb movement test robustly detected significant motor dysfunction at day 1, 3, and 7 post-injury that positively but inversely correlated with cortical volume at day 1 and 7 post-injury, respectively. The consistent observation of neuronal cell loss in the hemorrhagic core that subsequently extended to degeneration of neurons in the peri-hemorrhagic area, with accompanying motor abnormalities at least up to the subacute phase, advances this cortical hemorrhage model as a platform for examining the pathophysiology of and experimental treatments for ICH.
AB - The cortex is a key brain region vulnerable to intracerebral hemorrhage (ICH) associated with stroke and head trauma. Animal models of ICH, via blood or collagenase infusion, have been developed most commonly to target the striatum. Here, we show that stereotaxic injection of collagenase type IV into two sites of the right cortex of adult C57BL6 mice produced hemorrhage to the cortex, subcortical white matter and hippocampus at day 1 post-injury, followed by cortical volume decrement by day 7. Reductions in MAP2- and NeuN-positive neurons were detected at day 1 and 7 post-injury in the core and peri-hemorrhagic cortex, respectively. Fluoro-Jade positive degenerating neurons were observed at day 1 in the peri-hemorrhagic area. An aberrant aggregation of GFAP-positive astrocytes and a significant reduction in RIP-positive oligodendroglial cells were detected at day 7 post-injury in the cortical area. In addition, a significant decrement in retrogradely Cholera Toxin Subunit B-labeled corticospinal neurons was recognized at day 14 post-injury in the ipsilateral cortex. Among the behavioral tests employed, the pole climb movement test robustly detected significant motor dysfunction at day 1, 3, and 7 post-injury that positively but inversely correlated with cortical volume at day 1 and 7 post-injury, respectively. The consistent observation of neuronal cell loss in the hemorrhagic core that subsequently extended to degeneration of neurons in the peri-hemorrhagic area, with accompanying motor abnormalities at least up to the subacute phase, advances this cortical hemorrhage model as a platform for examining the pathophysiology of and experimental treatments for ICH.
KW - Behavior
KW - Head trauma
KW - Intracerebral hemorrhage
KW - Motor cortex
KW - Neurodegeneration
UR - http://www.scopus.com/inward/record.url?scp=77956610555&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77956610555&partnerID=8YFLogxK
U2 - 10.1016/j.brainres.2010.07.101
DO - 10.1016/j.brainres.2010.07.101
M3 - Article
C2 - 20691669
AN - SCOPUS:77956610555
VL - 1355
SP - 228
EP - 239
JO - Brain Research
JF - Brain Research
SN - 0006-8993
ER -