Peri-hemorrhagic degeneration accompanies stereotaxic collagenase-mediated cortical hemorrhage in mouse

Tadashi Masuda, Mina Maki, Koichi Hara, Takao Yasuhara, Noriyuki Matsukawa, Seongjin Yu, Eunkyung Cate Bae, Naoki Tajiri, Sonia H. Chheda, Marianna Aurora Solomita, Nathan Weinbren, Yuji Kaneko, Sergei A Kirov, David C Hess, Hideki Hida, Cesar V. Borlongan

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The cortex is a key brain region vulnerable to intracerebral hemorrhage (ICH) associated with stroke and head trauma. Animal models of ICH, via blood or collagenase infusion, have been developed most commonly to target the striatum. Here, we show that stereotaxic injection of collagenase type IV into two sites of the right cortex of adult C57BL6 mice produced hemorrhage to the cortex, subcortical white matter and hippocampus at day 1 post-injury, followed by cortical volume decrement by day 7. Reductions in MAP2- and NeuN-positive neurons were detected at day 1 and 7 post-injury in the core and peri-hemorrhagic cortex, respectively. Fluoro-Jade positive degenerating neurons were observed at day 1 in the peri-hemorrhagic area. An aberrant aggregation of GFAP-positive astrocytes and a significant reduction in RIP-positive oligodendroglial cells were detected at day 7 post-injury in the cortical area. In addition, a significant decrement in retrogradely Cholera Toxin Subunit B-labeled corticospinal neurons was recognized at day 14 post-injury in the ipsilateral cortex. Among the behavioral tests employed, the pole climb movement test robustly detected significant motor dysfunction at day 1, 3, and 7 post-injury that positively but inversely correlated with cortical volume at day 1 and 7 post-injury, respectively. The consistent observation of neuronal cell loss in the hemorrhagic core that subsequently extended to degeneration of neurons in the peri-hemorrhagic area, with accompanying motor abnormalities at least up to the subacute phase, advances this cortical hemorrhage model as a platform for examining the pathophysiology of and experimental treatments for ICH.

Original languageEnglish (US)
Pages (from-to)228-239
Number of pages12
JournalBrain Research
Volume1355
DOIs
StatePublished - Oct 8 2010

Fingerprint

Collagenases
Hemorrhage
Cerebral Hemorrhage
Wounds and Injuries
Neurons
Nerve Degeneration
Cholera Toxin
Craniocerebral Trauma
Astrocytes
Hippocampus
Animal Models
Stroke
Observation
Injections
Brain

Keywords

  • Behavior
  • Head trauma
  • Intracerebral hemorrhage
  • Motor cortex
  • Neurodegeneration

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Masuda, T., Maki, M., Hara, K., Yasuhara, T., Matsukawa, N., Yu, S., ... Borlongan, C. V. (2010). Peri-hemorrhagic degeneration accompanies stereotaxic collagenase-mediated cortical hemorrhage in mouse. Brain Research, 1355, 228-239. https://doi.org/10.1016/j.brainres.2010.07.101

Peri-hemorrhagic degeneration accompanies stereotaxic collagenase-mediated cortical hemorrhage in mouse. / Masuda, Tadashi; Maki, Mina; Hara, Koichi; Yasuhara, Takao; Matsukawa, Noriyuki; Yu, Seongjin; Bae, Eunkyung Cate; Tajiri, Naoki; Chheda, Sonia H.; Solomita, Marianna Aurora; Weinbren, Nathan; Kaneko, Yuji; Kirov, Sergei A; Hess, David C; Hida, Hideki; Borlongan, Cesar V.

In: Brain Research, Vol. 1355, 08.10.2010, p. 228-239.

Research output: Contribution to journalArticle

Masuda, T, Maki, M, Hara, K, Yasuhara, T, Matsukawa, N, Yu, S, Bae, EC, Tajiri, N, Chheda, SH, Solomita, MA, Weinbren, N, Kaneko, Y, Kirov, SA, Hess, DC, Hida, H & Borlongan, CV 2010, 'Peri-hemorrhagic degeneration accompanies stereotaxic collagenase-mediated cortical hemorrhage in mouse', Brain Research, vol. 1355, pp. 228-239. https://doi.org/10.1016/j.brainres.2010.07.101
Masuda, Tadashi ; Maki, Mina ; Hara, Koichi ; Yasuhara, Takao ; Matsukawa, Noriyuki ; Yu, Seongjin ; Bae, Eunkyung Cate ; Tajiri, Naoki ; Chheda, Sonia H. ; Solomita, Marianna Aurora ; Weinbren, Nathan ; Kaneko, Yuji ; Kirov, Sergei A ; Hess, David C ; Hida, Hideki ; Borlongan, Cesar V. / Peri-hemorrhagic degeneration accompanies stereotaxic collagenase-mediated cortical hemorrhage in mouse. In: Brain Research. 2010 ; Vol. 1355. pp. 228-239.
@article{c676a01372ab4b50b2634a50ac0d5b30,
title = "Peri-hemorrhagic degeneration accompanies stereotaxic collagenase-mediated cortical hemorrhage in mouse",
abstract = "The cortex is a key brain region vulnerable to intracerebral hemorrhage (ICH) associated with stroke and head trauma. Animal models of ICH, via blood or collagenase infusion, have been developed most commonly to target the striatum. Here, we show that stereotaxic injection of collagenase type IV into two sites of the right cortex of adult C57BL6 mice produced hemorrhage to the cortex, subcortical white matter and hippocampus at day 1 post-injury, followed by cortical volume decrement by day 7. Reductions in MAP2- and NeuN-positive neurons were detected at day 1 and 7 post-injury in the core and peri-hemorrhagic cortex, respectively. Fluoro-Jade positive degenerating neurons were observed at day 1 in the peri-hemorrhagic area. An aberrant aggregation of GFAP-positive astrocytes and a significant reduction in RIP-positive oligodendroglial cells were detected at day 7 post-injury in the cortical area. In addition, a significant decrement in retrogradely Cholera Toxin Subunit B-labeled corticospinal neurons was recognized at day 14 post-injury in the ipsilateral cortex. Among the behavioral tests employed, the pole climb movement test robustly detected significant motor dysfunction at day 1, 3, and 7 post-injury that positively but inversely correlated with cortical volume at day 1 and 7 post-injury, respectively. The consistent observation of neuronal cell loss in the hemorrhagic core that subsequently extended to degeneration of neurons in the peri-hemorrhagic area, with accompanying motor abnormalities at least up to the subacute phase, advances this cortical hemorrhage model as a platform for examining the pathophysiology of and experimental treatments for ICH.",
keywords = "Behavior, Head trauma, Intracerebral hemorrhage, Motor cortex, Neurodegeneration",
author = "Tadashi Masuda and Mina Maki and Koichi Hara and Takao Yasuhara and Noriyuki Matsukawa and Seongjin Yu and Bae, {Eunkyung Cate} and Naoki Tajiri and Chheda, {Sonia H.} and Solomita, {Marianna Aurora} and Nathan Weinbren and Yuji Kaneko and Kirov, {Sergei A} and Hess, {David C} and Hideki Hida and Borlongan, {Cesar V.}",
year = "2010",
month = "10",
day = "8",
doi = "10.1016/j.brainres.2010.07.101",
language = "English (US)",
volume = "1355",
pages = "228--239",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",

}

TY - JOUR

T1 - Peri-hemorrhagic degeneration accompanies stereotaxic collagenase-mediated cortical hemorrhage in mouse

AU - Masuda, Tadashi

AU - Maki, Mina

AU - Hara, Koichi

AU - Yasuhara, Takao

AU - Matsukawa, Noriyuki

AU - Yu, Seongjin

AU - Bae, Eunkyung Cate

AU - Tajiri, Naoki

AU - Chheda, Sonia H.

AU - Solomita, Marianna Aurora

AU - Weinbren, Nathan

AU - Kaneko, Yuji

AU - Kirov, Sergei A

AU - Hess, David C

AU - Hida, Hideki

AU - Borlongan, Cesar V.

PY - 2010/10/8

Y1 - 2010/10/8

N2 - The cortex is a key brain region vulnerable to intracerebral hemorrhage (ICH) associated with stroke and head trauma. Animal models of ICH, via blood or collagenase infusion, have been developed most commonly to target the striatum. Here, we show that stereotaxic injection of collagenase type IV into two sites of the right cortex of adult C57BL6 mice produced hemorrhage to the cortex, subcortical white matter and hippocampus at day 1 post-injury, followed by cortical volume decrement by day 7. Reductions in MAP2- and NeuN-positive neurons were detected at day 1 and 7 post-injury in the core and peri-hemorrhagic cortex, respectively. Fluoro-Jade positive degenerating neurons were observed at day 1 in the peri-hemorrhagic area. An aberrant aggregation of GFAP-positive astrocytes and a significant reduction in RIP-positive oligodendroglial cells were detected at day 7 post-injury in the cortical area. In addition, a significant decrement in retrogradely Cholera Toxin Subunit B-labeled corticospinal neurons was recognized at day 14 post-injury in the ipsilateral cortex. Among the behavioral tests employed, the pole climb movement test robustly detected significant motor dysfunction at day 1, 3, and 7 post-injury that positively but inversely correlated with cortical volume at day 1 and 7 post-injury, respectively. The consistent observation of neuronal cell loss in the hemorrhagic core that subsequently extended to degeneration of neurons in the peri-hemorrhagic area, with accompanying motor abnormalities at least up to the subacute phase, advances this cortical hemorrhage model as a platform for examining the pathophysiology of and experimental treatments for ICH.

AB - The cortex is a key brain region vulnerable to intracerebral hemorrhage (ICH) associated with stroke and head trauma. Animal models of ICH, via blood or collagenase infusion, have been developed most commonly to target the striatum. Here, we show that stereotaxic injection of collagenase type IV into two sites of the right cortex of adult C57BL6 mice produced hemorrhage to the cortex, subcortical white matter and hippocampus at day 1 post-injury, followed by cortical volume decrement by day 7. Reductions in MAP2- and NeuN-positive neurons were detected at day 1 and 7 post-injury in the core and peri-hemorrhagic cortex, respectively. Fluoro-Jade positive degenerating neurons were observed at day 1 in the peri-hemorrhagic area. An aberrant aggregation of GFAP-positive astrocytes and a significant reduction in RIP-positive oligodendroglial cells were detected at day 7 post-injury in the cortical area. In addition, a significant decrement in retrogradely Cholera Toxin Subunit B-labeled corticospinal neurons was recognized at day 14 post-injury in the ipsilateral cortex. Among the behavioral tests employed, the pole climb movement test robustly detected significant motor dysfunction at day 1, 3, and 7 post-injury that positively but inversely correlated with cortical volume at day 1 and 7 post-injury, respectively. The consistent observation of neuronal cell loss in the hemorrhagic core that subsequently extended to degeneration of neurons in the peri-hemorrhagic area, with accompanying motor abnormalities at least up to the subacute phase, advances this cortical hemorrhage model as a platform for examining the pathophysiology of and experimental treatments for ICH.

KW - Behavior

KW - Head trauma

KW - Intracerebral hemorrhage

KW - Motor cortex

KW - Neurodegeneration

UR - http://www.scopus.com/inward/record.url?scp=77956610555&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77956610555&partnerID=8YFLogxK

U2 - 10.1016/j.brainres.2010.07.101

DO - 10.1016/j.brainres.2010.07.101

M3 - Article

VL - 1355

SP - 228

EP - 239

JO - Brain Research

JF - Brain Research

SN - 0006-8993

ER -