Periodontal and endodontic pathology delays extraction socket healing in a canine model

Jung Hoon Kim, Ki Tae Koo, Joseph Capetillo, Jung Ju Kim, Jung Min Yoo, Heithem Ben Amara, Jung Chul Park, Frank Schwarz, Ulf M.E. Wikesjö

Research output: Contribution to journalArticle

Abstract

Purpose: The aim of the present exploratory study was to evaluate extraction socket healing at sites with a history of periodontal and endodontic pathology. Methods: The mandibular 4th premolar teeth in 5 adult beagle dogs served as experimental units. Periodontal and endodontic lesions were induced in 1 premolar site in each animal using wire ligatures and pulpal exposure over 3 months (diseased sites). The contralateral premolar sites served as healthy controls. The mandibular 4th premolar teeth were then extracted with minimal trauma, followed by careful wound debridement. The animals were sacrificed at days 1, 7, 30, 60, and 90 post-extraction for analysis, and the healing patterns at the healthy and diseased extraction sites were compared using radiography, scanning electron microscopy, histology, and histometry. Results: During the first 7 days of healing, a significant presence of inflammatory granulation tissue was noted at the diseased sites (day 1), along with a slightly accelerated rate of fibrin clot resolution on day 7. On day 30, the diseased extraction sites showed a greater percentage of persistent fibrous connective tissue, and an absence of bone marrow formation. In contrast, healthy sites showed initial signs of bone marrow formation on day 30, and subsequently a significantly greater proportion of mature bone marrow formation on both days 60 and 90. Radiographs exhibited sclerotic changes adjoining apical endodontic lesions, with scanning electron microscopy showing collapsed Volkmann canals protruding from these regions in the diseased sites. Furthermore, periodontal ligament fibers exhibited a parallel orientation to the alveolar walls of the diseased sites, in contrast to a perpendicular arrangement in the healthy sites. Conclusions: Within the limitations of this study, it appears that a history of periodontal and endodontic pathology may critically affect bone formation and maturation, leading to delayed and compromised extraction socket healing.

Original languageEnglish (US)
Pages (from-to)143-153
Number of pages11
JournalJournal of Periodontal and Implant Science
Volume47
Issue number3
DOIs
StatePublished - Jun 1 2017

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Endodontics
Canidae
Bicuspid
Pathology
Osteogenesis
Bone Marrow
Electron Scanning Microscopy
Tooth
Haversian System
Periodontal Ligament
Granulation Tissue
Wounds and Injuries
Debridement
Fibrin
Radiography
Connective Tissue
Ligation
Histology
Dogs

Keywords

  • Biological models
  • Bone marrow
  • Histology
  • Wound healing

ASJC Scopus subject areas

  • Oral Surgery
  • Periodontics

Cite this

Kim, J. H., Koo, K. T., Capetillo, J., Kim, J. J., Yoo, J. M., Amara, H. B., ... Wikesjö, U. M. E. (2017). Periodontal and endodontic pathology delays extraction socket healing in a canine model. Journal of Periodontal and Implant Science, 47(3), 143-153. https://doi.org/10.5051/jpis.2017.47.3.143

Periodontal and endodontic pathology delays extraction socket healing in a canine model. / Kim, Jung Hoon; Koo, Ki Tae; Capetillo, Joseph; Kim, Jung Ju; Yoo, Jung Min; Amara, Heithem Ben; Park, Jung Chul; Schwarz, Frank; Wikesjö, Ulf M.E.

In: Journal of Periodontal and Implant Science, Vol. 47, No. 3, 01.06.2017, p. 143-153.

Research output: Contribution to journalArticle

Kim, JH, Koo, KT, Capetillo, J, Kim, JJ, Yoo, JM, Amara, HB, Park, JC, Schwarz, F & Wikesjö, UME 2017, 'Periodontal and endodontic pathology delays extraction socket healing in a canine model', Journal of Periodontal and Implant Science, vol. 47, no. 3, pp. 143-153. https://doi.org/10.5051/jpis.2017.47.3.143
Kim, Jung Hoon ; Koo, Ki Tae ; Capetillo, Joseph ; Kim, Jung Ju ; Yoo, Jung Min ; Amara, Heithem Ben ; Park, Jung Chul ; Schwarz, Frank ; Wikesjö, Ulf M.E. / Periodontal and endodontic pathology delays extraction socket healing in a canine model. In: Journal of Periodontal and Implant Science. 2017 ; Vol. 47, No. 3. pp. 143-153.
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abstract = "Purpose: The aim of the present exploratory study was to evaluate extraction socket healing at sites with a history of periodontal and endodontic pathology. Methods: The mandibular 4th premolar teeth in 5 adult beagle dogs served as experimental units. Periodontal and endodontic lesions were induced in 1 premolar site in each animal using wire ligatures and pulpal exposure over 3 months (diseased sites). The contralateral premolar sites served as healthy controls. The mandibular 4th premolar teeth were then extracted with minimal trauma, followed by careful wound debridement. The animals were sacrificed at days 1, 7, 30, 60, and 90 post-extraction for analysis, and the healing patterns at the healthy and diseased extraction sites were compared using radiography, scanning electron microscopy, histology, and histometry. Results: During the first 7 days of healing, a significant presence of inflammatory granulation tissue was noted at the diseased sites (day 1), along with a slightly accelerated rate of fibrin clot resolution on day 7. On day 30, the diseased extraction sites showed a greater percentage of persistent fibrous connective tissue, and an absence of bone marrow formation. In contrast, healthy sites showed initial signs of bone marrow formation on day 30, and subsequently a significantly greater proportion of mature bone marrow formation on both days 60 and 90. Radiographs exhibited sclerotic changes adjoining apical endodontic lesions, with scanning electron microscopy showing collapsed Volkmann canals protruding from these regions in the diseased sites. Furthermore, periodontal ligament fibers exhibited a parallel orientation to the alveolar walls of the diseased sites, in contrast to a perpendicular arrangement in the healthy sites. Conclusions: Within the limitations of this study, it appears that a history of periodontal and endodontic pathology may critically affect bone formation and maturation, leading to delayed and compromised extraction socket healing.",
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