Periodontal microbiota in patients with coronary artery disease measured by real-time polymerase chain reaction: A case-control study

Claudia Nonnenmacher, Michael Stelzel, Cristiano Susin, Alexander M. Sattler, Juergen R. Schaefer, Bernhard Maisch, Reinier Mutters, Lavin Flores-de-Jacoby

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37 Scopus citations

Abstract

Background: Recent data have shown that periodontal disease may increase the risk of occurrence of coronary heart disease in which inflammation initiated by bacteria and their compounds might be a common causal factor. This case-control study aimed at studying the relationship between periodontal disease and coronary artery disease (CAD) based on clinical and periodontal microbiologic parameters. Methods: A total of 90 male subjects, 48 to 80 years of age, were included in this study. Forty-five men had CAD (CAD+), which was confirmed by coronary angiography. Forty-five age-matched controls showed no history or symptoms of CAD (CAD-). All subjects underwent a clinical periodontal examination including assessment of tooth loss, probing depth, clinical attachment level, and bleeding on probing. In the CAD+ group, this examination took place 1 day before coronary angiography. Subgingival microbial samples were taken and evaluated by means of real-time polymerase chain reaction (RT-PCR) for the total amount of bacteria and the following periodontopathogens: Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Pauimonas micra (formerly Micromonas micros), Dialister pneumosintes, and Campylobacter rectus. Results: Compared to control subjects, CAD+ subjects had significantly deeper pockets (2.28 mm versus 2.96 mm; P<0.001) and greater attachment loss (2.85 mm versus 3.65 mm; P<0.001), and this difference remained statistically significant after adjusting for smoking. No significant differences were observed between cases and controls with regard to the number of teeth present. P. intermedia was the only periodontal pathogen that showed significantly higher mean counts in CAD+ subjects compared to CAD- subjects. Higher counts of total bacteria, P. micra, D. pneumosintes, and C. rectus were found in the CAD- group. Conclusion: The results suggest that a relationship between periodontal disease and coronary heart disease exists, although P. intermedia was the only periodontopathogen related to CAD.

Original languageEnglish (US)
Pages (from-to)1724-1730
Number of pages7
JournalJournal of periodontology
Volume78
Issue number9
DOIs
StatePublished - Sep 1 2007

Keywords

  • Coronary artery disease
  • Periodontal disease
  • Plaque
  • Polymerase chain reaction, real time

ASJC Scopus subject areas

  • Periodontics

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    Nonnenmacher, C., Stelzel, M., Susin, C., Sattler, A. M., Schaefer, J. R., Maisch, B., Mutters, R., & Flores-de-Jacoby, L. (2007). Periodontal microbiota in patients with coronary artery disease measured by real-time polymerase chain reaction: A case-control study. Journal of periodontology, 78(9), 1724-1730. https://doi.org/10.1902/jop.2007.060345