Periodontal microbiota in patients with coronary artery disease measured by real-time polymerase chain reaction: A case-control study

Claudia Nonnenmacher, Michael Stelzel, Cristiano Susin, Alexander M. Sattler, Juergen R. Schaefer, Bernhard Maisch, Reinier Mutters, Lavin Flores-de-Jacoby

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Background: Recent data have shown that periodontal disease may increase the risk of occurrence of coronary heart disease in which inflammation initiated by bacteria and their compounds might be a common causal factor. This case-control study aimed at studying the relationship between periodontal disease and coronary artery disease (CAD) based on clinical and periodontal microbiologic parameters. Methods: A total of 90 male subjects, 48 to 80 years of age, were included in this study. Forty-five men had CAD (CAD+), which was confirmed by coronary angiography. Forty-five age-matched controls showed no history or symptoms of CAD (CAD-). All subjects underwent a clinical periodontal examination including assessment of tooth loss, probing depth, clinical attachment level, and bleeding on probing. In the CAD+ group, this examination took place 1 day before coronary angiography. Subgingival microbial samples were taken and evaluated by means of real-time polymerase chain reaction (RT-PCR) for the total amount of bacteria and the following periodontopathogens: Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Pauimonas micra (formerly Micromonas micros), Dialister pneumosintes, and Campylobacter rectus. Results: Compared to control subjects, CAD+ subjects had significantly deeper pockets (2.28 mm versus 2.96 mm; P<0.001) and greater attachment loss (2.85 mm versus 3.65 mm; P<0.001), and this difference remained statistically significant after adjusting for smoking. No significant differences were observed between cases and controls with regard to the number of teeth present. P. intermedia was the only periodontal pathogen that showed significantly higher mean counts in CAD+ subjects compared to CAD- subjects. Higher counts of total bacteria, P. micra, D. pneumosintes, and C. rectus were found in the CAD- group. Conclusion: The results suggest that a relationship between periodontal disease and coronary heart disease exists, although P. intermedia was the only periodontopathogen related to CAD.

Original languageEnglish (US)
Pages (from-to)1724-1730
Number of pages7
JournalJournal of periodontology
Volume78
Issue number9
DOIs
StatePublished - Sep 1 2007

Fingerprint

Microbiota
Case-Control Studies
Real-Time Polymerase Chain Reaction
Coronary Artery Disease
Prevotella intermedia
Campylobacter rectus
Periodontal Diseases
Coronary Angiography
Bacteria
Coronary Disease
Aggregatibacter actinomycetemcomitans
Tooth Loss
Porphyromonas gingivalis
Tooth
Smoking
History
Hemorrhage
Inflammation

Keywords

  • Coronary artery disease
  • Periodontal disease
  • Plaque
  • Polymerase chain reaction, real time

ASJC Scopus subject areas

  • Periodontics

Cite this

Nonnenmacher, C., Stelzel, M., Susin, C., Sattler, A. M., Schaefer, J. R., Maisch, B., ... Flores-de-Jacoby, L. (2007). Periodontal microbiota in patients with coronary artery disease measured by real-time polymerase chain reaction: A case-control study. Journal of periodontology, 78(9), 1724-1730. https://doi.org/10.1902/jop.2007.060345

Periodontal microbiota in patients with coronary artery disease measured by real-time polymerase chain reaction : A case-control study. / Nonnenmacher, Claudia; Stelzel, Michael; Susin, Cristiano; Sattler, Alexander M.; Schaefer, Juergen R.; Maisch, Bernhard; Mutters, Reinier; Flores-de-Jacoby, Lavin.

In: Journal of periodontology, Vol. 78, No. 9, 01.09.2007, p. 1724-1730.

Research output: Contribution to journalArticle

Nonnenmacher, C, Stelzel, M, Susin, C, Sattler, AM, Schaefer, JR, Maisch, B, Mutters, R & Flores-de-Jacoby, L 2007, 'Periodontal microbiota in patients with coronary artery disease measured by real-time polymerase chain reaction: A case-control study', Journal of periodontology, vol. 78, no. 9, pp. 1724-1730. https://doi.org/10.1902/jop.2007.060345
Nonnenmacher, Claudia ; Stelzel, Michael ; Susin, Cristiano ; Sattler, Alexander M. ; Schaefer, Juergen R. ; Maisch, Bernhard ; Mutters, Reinier ; Flores-de-Jacoby, Lavin. / Periodontal microbiota in patients with coronary artery disease measured by real-time polymerase chain reaction : A case-control study. In: Journal of periodontology. 2007 ; Vol. 78, No. 9. pp. 1724-1730.
@article{0ef3c65b80544adb8d53a4fa8a52de36,
title = "Periodontal microbiota in patients with coronary artery disease measured by real-time polymerase chain reaction: A case-control study",
abstract = "Background: Recent data have shown that periodontal disease may increase the risk of occurrence of coronary heart disease in which inflammation initiated by bacteria and their compounds might be a common causal factor. This case-control study aimed at studying the relationship between periodontal disease and coronary artery disease (CAD) based on clinical and periodontal microbiologic parameters. Methods: A total of 90 male subjects, 48 to 80 years of age, were included in this study. Forty-five men had CAD (CAD+), which was confirmed by coronary angiography. Forty-five age-matched controls showed no history or symptoms of CAD (CAD-). All subjects underwent a clinical periodontal examination including assessment of tooth loss, probing depth, clinical attachment level, and bleeding on probing. In the CAD+ group, this examination took place 1 day before coronary angiography. Subgingival microbial samples were taken and evaluated by means of real-time polymerase chain reaction (RT-PCR) for the total amount of bacteria and the following periodontopathogens: Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Pauimonas micra (formerly Micromonas micros), Dialister pneumosintes, and Campylobacter rectus. Results: Compared to control subjects, CAD+ subjects had significantly deeper pockets (2.28 mm versus 2.96 mm; P<0.001) and greater attachment loss (2.85 mm versus 3.65 mm; P<0.001), and this difference remained statistically significant after adjusting for smoking. No significant differences were observed between cases and controls with regard to the number of teeth present. P. intermedia was the only periodontal pathogen that showed significantly higher mean counts in CAD+ subjects compared to CAD- subjects. Higher counts of total bacteria, P. micra, D. pneumosintes, and C. rectus were found in the CAD- group. Conclusion: The results suggest that a relationship between periodontal disease and coronary heart disease exists, although P. intermedia was the only periodontopathogen related to CAD.",
keywords = "Coronary artery disease, Periodontal disease, Plaque, Polymerase chain reaction, real time",
author = "Claudia Nonnenmacher and Michael Stelzel and Cristiano Susin and Sattler, {Alexander M.} and Schaefer, {Juergen R.} and Bernhard Maisch and Reinier Mutters and Lavin Flores-de-Jacoby",
year = "2007",
month = "9",
day = "1",
doi = "10.1902/jop.2007.060345",
language = "English (US)",
volume = "78",
pages = "1724--1730",
journal = "Journal of Periodontology",
issn = "0022-3492",
publisher = "American Academy of Periodontology",
number = "9",

}

TY - JOUR

T1 - Periodontal microbiota in patients with coronary artery disease measured by real-time polymerase chain reaction

T2 - A case-control study

AU - Nonnenmacher, Claudia

AU - Stelzel, Michael

AU - Susin, Cristiano

AU - Sattler, Alexander M.

AU - Schaefer, Juergen R.

AU - Maisch, Bernhard

AU - Mutters, Reinier

AU - Flores-de-Jacoby, Lavin

PY - 2007/9/1

Y1 - 2007/9/1

N2 - Background: Recent data have shown that periodontal disease may increase the risk of occurrence of coronary heart disease in which inflammation initiated by bacteria and their compounds might be a common causal factor. This case-control study aimed at studying the relationship between periodontal disease and coronary artery disease (CAD) based on clinical and periodontal microbiologic parameters. Methods: A total of 90 male subjects, 48 to 80 years of age, were included in this study. Forty-five men had CAD (CAD+), which was confirmed by coronary angiography. Forty-five age-matched controls showed no history or symptoms of CAD (CAD-). All subjects underwent a clinical periodontal examination including assessment of tooth loss, probing depth, clinical attachment level, and bleeding on probing. In the CAD+ group, this examination took place 1 day before coronary angiography. Subgingival microbial samples were taken and evaluated by means of real-time polymerase chain reaction (RT-PCR) for the total amount of bacteria and the following periodontopathogens: Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Pauimonas micra (formerly Micromonas micros), Dialister pneumosintes, and Campylobacter rectus. Results: Compared to control subjects, CAD+ subjects had significantly deeper pockets (2.28 mm versus 2.96 mm; P<0.001) and greater attachment loss (2.85 mm versus 3.65 mm; P<0.001), and this difference remained statistically significant after adjusting for smoking. No significant differences were observed between cases and controls with regard to the number of teeth present. P. intermedia was the only periodontal pathogen that showed significantly higher mean counts in CAD+ subjects compared to CAD- subjects. Higher counts of total bacteria, P. micra, D. pneumosintes, and C. rectus were found in the CAD- group. Conclusion: The results suggest that a relationship between periodontal disease and coronary heart disease exists, although P. intermedia was the only periodontopathogen related to CAD.

AB - Background: Recent data have shown that periodontal disease may increase the risk of occurrence of coronary heart disease in which inflammation initiated by bacteria and their compounds might be a common causal factor. This case-control study aimed at studying the relationship between periodontal disease and coronary artery disease (CAD) based on clinical and periodontal microbiologic parameters. Methods: A total of 90 male subjects, 48 to 80 years of age, were included in this study. Forty-five men had CAD (CAD+), which was confirmed by coronary angiography. Forty-five age-matched controls showed no history or symptoms of CAD (CAD-). All subjects underwent a clinical periodontal examination including assessment of tooth loss, probing depth, clinical attachment level, and bleeding on probing. In the CAD+ group, this examination took place 1 day before coronary angiography. Subgingival microbial samples were taken and evaluated by means of real-time polymerase chain reaction (RT-PCR) for the total amount of bacteria and the following periodontopathogens: Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Pauimonas micra (formerly Micromonas micros), Dialister pneumosintes, and Campylobacter rectus. Results: Compared to control subjects, CAD+ subjects had significantly deeper pockets (2.28 mm versus 2.96 mm; P<0.001) and greater attachment loss (2.85 mm versus 3.65 mm; P<0.001), and this difference remained statistically significant after adjusting for smoking. No significant differences were observed between cases and controls with regard to the number of teeth present. P. intermedia was the only periodontal pathogen that showed significantly higher mean counts in CAD+ subjects compared to CAD- subjects. Higher counts of total bacteria, P. micra, D. pneumosintes, and C. rectus were found in the CAD- group. Conclusion: The results suggest that a relationship between periodontal disease and coronary heart disease exists, although P. intermedia was the only periodontopathogen related to CAD.

KW - Coronary artery disease

KW - Periodontal disease

KW - Plaque

KW - Polymerase chain reaction, real time

UR - http://www.scopus.com/inward/record.url?scp=34748901208&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34748901208&partnerID=8YFLogxK

U2 - 10.1902/jop.2007.060345

DO - 10.1902/jop.2007.060345

M3 - Article

C2 - 17760542

AN - SCOPUS:34748901208

VL - 78

SP - 1724

EP - 1730

JO - Journal of Periodontology

JF - Journal of Periodontology

SN - 0022-3492

IS - 9

ER -