Periodontal repair in dogs: Effect of recombinant human bone morphogenetic protein-12 (rhBMP-12) on regeneration of alveolar bone and periodontal attachment: A pilot study

Ulf M E Wikesjö, Rachel G. Sorensen, Atsuhiro Kinoshita, X. Jian Li, John M. Wozney

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

Objectives: Recombinant human bone morphogenetic protein-2 (rhBMP-2) has been shown to stimulate alveolar bone and cementum formation in periodontal defects but not a functionally oriented periodontal ligament (PDL). Subcutaneous and intramuscular implants of BMP-12 have been shown to induce tendon formation and ligament-like tissue. The objective of this study was to evaluate rhBMP-12 for periodontal regeneration, in particular PDL formation. Methods: Six young adult Hound Labrador mongrel dogs were used. Routine supraalveolar periodontal defects were created around the mandibular premolar teeth. Three animals received rhBMP-12(0.04 mg/ml) in an absorbable collagen sponge (ACS) carrier vs. rhBMP-12(0.2 mg/mL)/ACS in contralateral defects. Three animals received rhBMP-12(1.0 mg/ml)/ACS vs. rhBMP-2(0.2 mg/ml)/ACS (total implant volume/defect ∼1 ml). The animals were euthanized 8 weeks postsurgery and block biopsies were processed for histometric analysis. Results: Bone regeneration appeared increased in sites receiving rhBMP-2/ACS compared to sites receiving rhBMP-12/ACS. Cementum regeneration was similar comparing sites implanted with rhBMP-2/ACS to sites implanted with rhBMP-12/ ACS. In contrast, sites receiving rhBMP-12/ACS exhibited a functionally oriented PDL bridging the gap between newly formed bone and cementum whereas this was a rare observation in sites receiving rhBMP-2/ACS. Ankylosis appeared increased in sites receiving rhBMP-2/ACS compared to those receiving rhBMP-12/ACS. Conclusions: The outcomes of this study suggest that rhBMP-12 may have significant effects on regeneration of the PDL. Additional preclinical evaluation is needed to confirm these initial observations prior to clinical application.

Original languageEnglish (US)
Pages (from-to)662-670
Number of pages9
JournalJournal of Clinical Periodontology
Volume31
Issue number8
DOIs
StatePublished - Aug 1 2004

Fingerprint

Bone Regeneration
Porifera
Collagen
Dogs
Periodontal Ligament
Dental Cementum
Regeneration
human GDF7 protein
Newfoundland and Labrador
Ankylosis
Bicuspid
Ligaments
Osteogenesis
Tendons
recombinant human bone morphogenetic protein-2
Young Adult
Tooth
Observation
Outcome Assessment (Health Care)

Keywords

  • Cementum
  • Dogs
  • Periodontal ligament
  • Periodontal regeneration
  • Tissue engineering

ASJC Scopus subject areas

  • Periodontics

Cite this

Periodontal repair in dogs : Effect of recombinant human bone morphogenetic protein-12 (rhBMP-12) on regeneration of alveolar bone and periodontal attachment: A pilot study. / Wikesjö, Ulf M E; Sorensen, Rachel G.; Kinoshita, Atsuhiro; Li, X. Jian; Wozney, John M.

In: Journal of Clinical Periodontology, Vol. 31, No. 8, 01.08.2004, p. 662-670.

Research output: Contribution to journalArticle

Wikesjö, Ulf M E ; Sorensen, Rachel G. ; Kinoshita, Atsuhiro ; Li, X. Jian ; Wozney, John M. / Periodontal repair in dogs : Effect of recombinant human bone morphogenetic protein-12 (rhBMP-12) on regeneration of alveolar bone and periodontal attachment: A pilot study. In: Journal of Clinical Periodontology. 2004 ; Vol. 31, No. 8. pp. 662-670.
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AU - Wikesjö, Ulf M E

AU - Sorensen, Rachel G.

AU - Kinoshita, Atsuhiro

AU - Li, X. Jian

AU - Wozney, John M.

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AB - Objectives: Recombinant human bone morphogenetic protein-2 (rhBMP-2) has been shown to stimulate alveolar bone and cementum formation in periodontal defects but not a functionally oriented periodontal ligament (PDL). Subcutaneous and intramuscular implants of BMP-12 have been shown to induce tendon formation and ligament-like tissue. The objective of this study was to evaluate rhBMP-12 for periodontal regeneration, in particular PDL formation. Methods: Six young adult Hound Labrador mongrel dogs were used. Routine supraalveolar periodontal defects were created around the mandibular premolar teeth. Three animals received rhBMP-12(0.04 mg/ml) in an absorbable collagen sponge (ACS) carrier vs. rhBMP-12(0.2 mg/mL)/ACS in contralateral defects. Three animals received rhBMP-12(1.0 mg/ml)/ACS vs. rhBMP-2(0.2 mg/ml)/ACS (total implant volume/defect ∼1 ml). The animals were euthanized 8 weeks postsurgery and block biopsies were processed for histometric analysis. Results: Bone regeneration appeared increased in sites receiving rhBMP-2/ACS compared to sites receiving rhBMP-12/ACS. Cementum regeneration was similar comparing sites implanted with rhBMP-2/ACS to sites implanted with rhBMP-12/ ACS. In contrast, sites receiving rhBMP-12/ACS exhibited a functionally oriented PDL bridging the gap between newly formed bone and cementum whereas this was a rare observation in sites receiving rhBMP-2/ACS. Ankylosis appeared increased in sites receiving rhBMP-2/ACS compared to those receiving rhBMP-12/ACS. Conclusions: The outcomes of this study suggest that rhBMP-12 may have significant effects on regeneration of the PDL. Additional preclinical evaluation is needed to confirm these initial observations prior to clinical application.

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