Periodontal repair in dogs: Effect of recombinant human transforming growth factor-β1 on guided tissue regeneration

Ulf M E Wikesjö, Saghi S. Razi, Thorarinn J. Sigurdsson, Dimitris N. Tatakis, Michael B. Lee, Boonsri Ongpipattanakul, Tue Nguyen, Ross Hardwick

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

This study evaluated alveolar bone and cementum regeneration following surgical implantation of recombinant human transforming growth factor-β1 (rhTGF-β1) in conjunction with guided tissue regeneration (GTR). Supraalveolar, critical size, periodontal defects were surgically created around the 3rd and 4th mandibular premolar teeth in right and left jaw quadrants in 5 beagle dogs. Alternate jaw quadrants in consecutive animals received rhTGF-β1 in a CaCO3/ hydroxyethyl starch carrier with GTR, or carrier with GTR alone (control). 20μg of rhTGF-β1 in buffer solution was incorporated into approximately 0.8 ml of carrier for each defect scheduled to receive ThTGF-β1. Animals were sacrificed at week 4 postsurgery and tissue blocks were harvested and processed for histometric analysis. Clinical healing was generally uneventful. Minor membrane exposures were observed. Defects with membrane exposure displayed an inflammatory infiltrate underneath the membrane. Bone regeneration of trabecular nature, apparent in all animals, was generally limited to the very apical aspect of the defects. Cementum regeneration was limited without obvious differences between experimental conditions. Comparing rhTGF-β1 to control defects, statistically significant differences were found for area (1.8±0.4 and 1.3±0.6 mm2, respectively; p<0.05) and density (0.3±0.1 and 0.2±0.03, respectively; p<0.05) of alveolar bone regeneration. Observed differences are small and represent a clinically insignificant potential for enhanced regeneration in this preclinical model. Within the limitations of study, it may be concluded that rhTGF-β1 has a restricted potential to enhance alveolar bone regeneration in conjunction with GTR.

Original languageEnglish (US)
Pages (from-to)475-481
Number of pages7
JournalJournal of Clinical Periodontology
Volume25
Issue number6
DOIs
StatePublished - Jan 1 1998

Fingerprint

Guided Tissue Regeneration
Transforming Growth Factors
Bone Regeneration
Dogs
Dental Cementum
Jaw
Membranes
Regeneration
Bicuspid
Starch
Tooth
Buffers

Keywords

  • Alveolar bone
  • CaCO
  • Cementum
  • Growth factors
  • Guided tissue regeneration
  • Periodontal repair/regeneration
  • TGFβ

ASJC Scopus subject areas

  • Periodontics

Cite this

Wikesjö, U. M. E., Razi, S. S., Sigurdsson, T. J., Tatakis, D. N., Lee, M. B., Ongpipattanakul, B., ... Hardwick, R. (1998). Periodontal repair in dogs: Effect of recombinant human transforming growth factor-β1 on guided tissue regeneration. Journal of Clinical Periodontology, 25(6), 475-481. https://doi.org/10.1111/j.1600-051X.1998.tb02476.x

Periodontal repair in dogs : Effect of recombinant human transforming growth factor-β1 on guided tissue regeneration. / Wikesjö, Ulf M E; Razi, Saghi S.; Sigurdsson, Thorarinn J.; Tatakis, Dimitris N.; Lee, Michael B.; Ongpipattanakul, Boonsri; Nguyen, Tue; Hardwick, Ross.

In: Journal of Clinical Periodontology, Vol. 25, No. 6, 01.01.1998, p. 475-481.

Research output: Contribution to journalArticle

Wikesjö, UME, Razi, SS, Sigurdsson, TJ, Tatakis, DN, Lee, MB, Ongpipattanakul, B, Nguyen, T & Hardwick, R 1998, 'Periodontal repair in dogs: Effect of recombinant human transforming growth factor-β1 on guided tissue regeneration', Journal of Clinical Periodontology, vol. 25, no. 6, pp. 475-481. https://doi.org/10.1111/j.1600-051X.1998.tb02476.x
Wikesjö, Ulf M E ; Razi, Saghi S. ; Sigurdsson, Thorarinn J. ; Tatakis, Dimitris N. ; Lee, Michael B. ; Ongpipattanakul, Boonsri ; Nguyen, Tue ; Hardwick, Ross. / Periodontal repair in dogs : Effect of recombinant human transforming growth factor-β1 on guided tissue regeneration. In: Journal of Clinical Periodontology. 1998 ; Vol. 25, No. 6. pp. 475-481.
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