Periodontal repair in dogs: Effect of rhBMP-2 concentration on regeneration of alveolar bone and periodontal attachment

Ulf M E Wikesjö, Paola Guglielmoni, Ananya Promsudthi, Kyoo Sung Cho, Leonardo Trombelli, Knut A. Selvig, Lisa Jin, John M. Wozney

Research output: Contribution to journalArticle

142 Scopus citations

Abstract

The objective of this study was to evaluate the effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) concentration on regeneration of alveolar bone and cementum, and on associated root resorption and ankylosis. Contralateral, critical size, supra-alveolar, periodontal defects were surgically produced and immediately implanted with rhBMP-2 in an absorbable collagen sponge (ACS) carrier in 8, young adult, male, beagle dogs. 6 animals received rhBMP-2/ACS (rhBMP-2 at 0.05, 0.10, or 0.20 mg/mL; total construct volume/ defect ∼4.0 mL) in contralateral defects following an incomplete block design. 2 animals received rhBMP-2/ACS (rhBMP-2 at 0 and 0.10 mg/mL) in contralateral defects (controls). The animals were euthanised at 8 weeks post-surgery and block sections of the defects were collected for histologic and histometric analysis. Supra-alveolar periodontal defects receiving rhBMP-2 at 0.05, 0.10, or 0.20 mg/ml exhibited extensive alveolar regeneration comprising 86%, 96%, and 88% of the defect height, respectively. Cementum regeneration encompassed 8%, 6%, and 8% of the defect height, respectively. Root resorption was observed for all rhBMP-2 concentrations. Ankylosis was observed in almost all teeth receiving rhBMP-2. Control defects without rhBMP-2 exhibited limited, if any, evidence of alveolar bone and cementum regeneration, root resorption, or ankylosis. Within the selected rhBMP-2 concentration and observation interval, there appear to be no meaningful differences in regeneration of alveolar bone and cementum. There also appear to be no significant differences in the incidence and extent of root resorption and ankylosis, though there may be a positive correlation with rhBMP-2 concentration.

Original languageEnglish (US)
Pages (from-to)392-400
Number of pages9
JournalJournal of Clinical Periodontology
Volume26
Issue number6
DOIs
StatePublished - Jun 1999

Keywords

  • Dogs
  • Growth factors
  • Periodontal defects
  • Wound healing

ASJC Scopus subject areas

  • Periodontics

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    Wikesjö, U. M. E., Guglielmoni, P., Promsudthi, A., Cho, K. S., Trombelli, L., Selvig, K. A., Jin, L., & Wozney, J. M. (1999). Periodontal repair in dogs: Effect of rhBMP-2 concentration on regeneration of alveolar bone and periodontal attachment. Journal of Clinical Periodontology, 26(6), 392-400. https://doi.org/10.1034/j.1600-051X.1999.260610.x