Periodontal repair in dogs: Guided tissue regeneration enhances bone formation in sites implanted with a coral-derived calcium carbonate biomaterial

Ki Tae Koo, Giuseppe Polimeni, Mohammed Qahash, Chong Kwan Kim, Ulf M E Wikesjö

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Background: Previous studies suggest that a bioresorbable calcium carbonate coral implant (CI) supports space provision and bone formation for guided tissue regeneration (GTR). However, it could not be discerned whether observed effects were because of GTR or whether the CI possessed osteoconductive properties enhancing bone formation. The objective of this study was to evaluate bone formation associated with the CI biomaterial in the presence and absence of provisions for GTR. Methods: Routine, critical size, 6 mm, supra-alveolar periodontal defects were created in 12 young adult Beagle dogs. Five animals received the CI alone (Biocoral® 1000). Seven animals received the CI/GTR combination using an expanded polytetrafluoroethylene barrier (GORE-TEX® Regenerative Material). The animals were euthanized at 4 weeks postsurgery and tissue blocks of the experimental sites were collected and processed for histometric analysis. Results: Clinical healing was uneventful. The histopathologic and histometric analysis revealed significantly increased bone formation (height and area) in sites receiving the CI/GTR combination compared with CI alone (2.3 ± 0.6 versus 1.2 ± 0.9mm; and 3.1 ± 0.8 versus 1.2 ± 1.1 mm2; p<0.05). The CI biomaterial appeared to be mostly unassociated with new bone formation; the CI particles were observed sequestered in newly formed bone, fibrovascular marrow, and in the supraalveolar connective tissue. Cementum formation was limited and observed in few sites for both treatment protocols. Conclusion: While GTR promoted new bone formation, the CI contributed limited, if any, osteoconductive effects.

Original languageEnglish (US)
Pages (from-to)104-110
Number of pages7
JournalJournal of Clinical Periodontology
Volume32
Issue number1
DOIs
StatePublished - Jan 1 2005

Fingerprint

Guided Tissue Regeneration
Anthozoa
Calcium Carbonate
Biocompatible Materials
Osteogenesis
Dogs
Polytetrafluoroethylene
Dental Cementum
Clinical Protocols
Connective Tissue
Young Adult
Bone Marrow

Keywords

  • Bone regeneration
  • Coral biomaterial
  • Dogs
  • Guided tissue regeneration
  • Tissue engineering

ASJC Scopus subject areas

  • Periodontics

Cite this

Periodontal repair in dogs : Guided tissue regeneration enhances bone formation in sites implanted with a coral-derived calcium carbonate biomaterial. / Koo, Ki Tae; Polimeni, Giuseppe; Qahash, Mohammed; Kim, Chong Kwan; Wikesjö, Ulf M E.

In: Journal of Clinical Periodontology, Vol. 32, No. 1, 01.01.2005, p. 104-110.

Research output: Contribution to journalArticle

Koo, Ki Tae ; Polimeni, Giuseppe ; Qahash, Mohammed ; Kim, Chong Kwan ; Wikesjö, Ulf M E. / Periodontal repair in dogs : Guided tissue regeneration enhances bone formation in sites implanted with a coral-derived calcium carbonate biomaterial. In: Journal of Clinical Periodontology. 2005 ; Vol. 32, No. 1. pp. 104-110.
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abstract = "Background: Previous studies suggest that a bioresorbable calcium carbonate coral implant (CI) supports space provision and bone formation for guided tissue regeneration (GTR). However, it could not be discerned whether observed effects were because of GTR or whether the CI possessed osteoconductive properties enhancing bone formation. The objective of this study was to evaluate bone formation associated with the CI biomaterial in the presence and absence of provisions for GTR. Methods: Routine, critical size, 6 mm, supra-alveolar periodontal defects were created in 12 young adult Beagle dogs. Five animals received the CI alone (Biocoral{\circledR} 1000). Seven animals received the CI/GTR combination using an expanded polytetrafluoroethylene barrier (GORE-TEX{\circledR} Regenerative Material). The animals were euthanized at 4 weeks postsurgery and tissue blocks of the experimental sites were collected and processed for histometric analysis. Results: Clinical healing was uneventful. The histopathologic and histometric analysis revealed significantly increased bone formation (height and area) in sites receiving the CI/GTR combination compared with CI alone (2.3 ± 0.6 versus 1.2 ± 0.9mm; and 3.1 ± 0.8 versus 1.2 ± 1.1 mm2; p<0.05). The CI biomaterial appeared to be mostly unassociated with new bone formation; the CI particles were observed sequestered in newly formed bone, fibrovascular marrow, and in the supraalveolar connective tissue. Cementum formation was limited and observed in few sites for both treatment protocols. Conclusion: While GTR promoted new bone formation, the CI contributed limited, if any, osteoconductive effects.",
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AU - Wikesjö, Ulf M E

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N2 - Background: Previous studies suggest that a bioresorbable calcium carbonate coral implant (CI) supports space provision and bone formation for guided tissue regeneration (GTR). However, it could not be discerned whether observed effects were because of GTR or whether the CI possessed osteoconductive properties enhancing bone formation. The objective of this study was to evaluate bone formation associated with the CI biomaterial in the presence and absence of provisions for GTR. Methods: Routine, critical size, 6 mm, supra-alveolar periodontal defects were created in 12 young adult Beagle dogs. Five animals received the CI alone (Biocoral® 1000). Seven animals received the CI/GTR combination using an expanded polytetrafluoroethylene barrier (GORE-TEX® Regenerative Material). The animals were euthanized at 4 weeks postsurgery and tissue blocks of the experimental sites were collected and processed for histometric analysis. Results: Clinical healing was uneventful. The histopathologic and histometric analysis revealed significantly increased bone formation (height and area) in sites receiving the CI/GTR combination compared with CI alone (2.3 ± 0.6 versus 1.2 ± 0.9mm; and 3.1 ± 0.8 versus 1.2 ± 1.1 mm2; p<0.05). The CI biomaterial appeared to be mostly unassociated with new bone formation; the CI particles were observed sequestered in newly formed bone, fibrovascular marrow, and in the supraalveolar connective tissue. Cementum formation was limited and observed in few sites for both treatment protocols. Conclusion: While GTR promoted new bone formation, the CI contributed limited, if any, osteoconductive effects.

AB - Background: Previous studies suggest that a bioresorbable calcium carbonate coral implant (CI) supports space provision and bone formation for guided tissue regeneration (GTR). However, it could not be discerned whether observed effects were because of GTR or whether the CI possessed osteoconductive properties enhancing bone formation. The objective of this study was to evaluate bone formation associated with the CI biomaterial in the presence and absence of provisions for GTR. Methods: Routine, critical size, 6 mm, supra-alveolar periodontal defects were created in 12 young adult Beagle dogs. Five animals received the CI alone (Biocoral® 1000). Seven animals received the CI/GTR combination using an expanded polytetrafluoroethylene barrier (GORE-TEX® Regenerative Material). The animals were euthanized at 4 weeks postsurgery and tissue blocks of the experimental sites were collected and processed for histometric analysis. Results: Clinical healing was uneventful. The histopathologic and histometric analysis revealed significantly increased bone formation (height and area) in sites receiving the CI/GTR combination compared with CI alone (2.3 ± 0.6 versus 1.2 ± 0.9mm; and 3.1 ± 0.8 versus 1.2 ± 1.1 mm2; p<0.05). The CI biomaterial appeared to be mostly unassociated with new bone formation; the CI particles were observed sequestered in newly formed bone, fibrovascular marrow, and in the supraalveolar connective tissue. Cementum formation was limited and observed in few sites for both treatment protocols. Conclusion: While GTR promoted new bone formation, the CI contributed limited, if any, osteoconductive effects.

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