Periodontal repair in dogs: rhBMP-2 significantly enhances bone formation under provisions for guided tissue regeneration

Ulf M E Wikesjö, Andreas V. Xiropaidis, Robert C. Thomson, Alonzo D. Cook, Knut A. Selvig, W. Ross Hardwick

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Background: Recombinant human bone morphogenetic protein-2 (rhBMP-2) has been shown to support the regeneration of alveolar bone and periodontal attachment in surgically created periodontal defects and in defects with a history of dental plaque and calculus exposure. Periodontal regeneration has also been shown following guided tissue regeneration using space-providing expanded polytetrafluoroethylene (ePTFE) devices. The objective of this study was to evaluate the influence of rhBMP-2 on regeneration of alveolar bone and periodontal attachment used in conjunction with a space-providing ePTFE device. Methods: Routine, critical-size, 5-6 mm, supra-alveolar, periodontal defects were created around the third and fourth mandibular premolar teeth in four young adult Hound Labrador mongrel dogs. rhBMP-2 (0.2 mg/ml) in an absorbable collagen sponge (rhBMP-2/ACS) or buffer/ACS (control) implants were randomly assigned to be placed around the premolar teeth in the left and right jaw quadrants in subsequent animals. Space-providing ePTFE devices with 300-μm laser-drilled pores, 0.8 mm apart, were used to cover the rhBMP-2 and control implants. The gingival flaps were advanced for primary wound closure. The animals were euthanized at 8 weeks postsurgery for histologic and histometric analyses. Results: Bone regeneration and ankylosis were significantly increased in jaw quadrants receiving rhBMP-2/ACS compared to control (bone height 4.8 ± 0.3 versus 2.0 ± 0.2 mm, p = 0.001; bone area 10.9 ± 1.3 versus 1.4 ± 0.1 mm2; p = 0.009, and ankylosis 2.2 ± 0.2 versus 0.04 ± 0.7 mm; p = 0.01). No differences between groups were found for cementum regeneration and root resorption. Conclusions: rhBMP-2 significantly enhances regeneration of alveolar bone in conjunction with a space-providing, macroporous ePTFE device for GTR.

Original languageEnglish (US)
Pages (from-to)705-714
Number of pages10
JournalJournal of Clinical Periodontology
Volume30
Issue number8
DOIs
StatePublished - Aug 1 2003

Fingerprint

Guided Tissue Regeneration
Osteogenesis
Dogs
Bone Regeneration
Polytetrafluoroethylene
Ankylosis
Equipment and Supplies
Bicuspid
Jaw
Regeneration
Tooth
Dental Calculus
Root Resorption
Newfoundland and Labrador
Dental Cementum
Bone and Bones
Dental Plaque
Porifera
recombinant human bone morphogenetic protein-2
Young Adult

Keywords

  • Periodontal regeneration
  • Seroma
  • Tissue engineering
  • Wound healing

ASJC Scopus subject areas

  • Periodontics

Cite this

Periodontal repair in dogs : rhBMP-2 significantly enhances bone formation under provisions for guided tissue regeneration. / Wikesjö, Ulf M E; Xiropaidis, Andreas V.; Thomson, Robert C.; Cook, Alonzo D.; Selvig, Knut A.; Hardwick, W. Ross.

In: Journal of Clinical Periodontology, Vol. 30, No. 8, 01.08.2003, p. 705-714.

Research output: Contribution to journalArticle

Wikesjö, Ulf M E ; Xiropaidis, Andreas V. ; Thomson, Robert C. ; Cook, Alonzo D. ; Selvig, Knut A. ; Hardwick, W. Ross. / Periodontal repair in dogs : rhBMP-2 significantly enhances bone formation under provisions for guided tissue regeneration. In: Journal of Clinical Periodontology. 2003 ; Vol. 30, No. 8. pp. 705-714.
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abstract = "Background: Recombinant human bone morphogenetic protein-2 (rhBMP-2) has been shown to support the regeneration of alveolar bone and periodontal attachment in surgically created periodontal defects and in defects with a history of dental plaque and calculus exposure. Periodontal regeneration has also been shown following guided tissue regeneration using space-providing expanded polytetrafluoroethylene (ePTFE) devices. The objective of this study was to evaluate the influence of rhBMP-2 on regeneration of alveolar bone and periodontal attachment used in conjunction with a space-providing ePTFE device. Methods: Routine, critical-size, 5-6 mm, supra-alveolar, periodontal defects were created around the third and fourth mandibular premolar teeth in four young adult Hound Labrador mongrel dogs. rhBMP-2 (0.2 mg/ml) in an absorbable collagen sponge (rhBMP-2/ACS) or buffer/ACS (control) implants were randomly assigned to be placed around the premolar teeth in the left and right jaw quadrants in subsequent animals. Space-providing ePTFE devices with 300-μm laser-drilled pores, 0.8 mm apart, were used to cover the rhBMP-2 and control implants. The gingival flaps were advanced for primary wound closure. The animals were euthanized at 8 weeks postsurgery for histologic and histometric analyses. Results: Bone regeneration and ankylosis were significantly increased in jaw quadrants receiving rhBMP-2/ACS compared to control (bone height 4.8 ± 0.3 versus 2.0 ± 0.2 mm, p = 0.001; bone area 10.9 ± 1.3 versus 1.4 ± 0.1 mm2; p = 0.009, and ankylosis 2.2 ± 0.2 versus 0.04 ± 0.7 mm; p = 0.01). No differences between groups were found for cementum regeneration and root resorption. Conclusions: rhBMP-2 significantly enhances regeneration of alveolar bone in conjunction with a space-providing, macroporous ePTFE device for GTR.",
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T2 - rhBMP-2 significantly enhances bone formation under provisions for guided tissue regeneration

AU - Wikesjö, Ulf M E

AU - Xiropaidis, Andreas V.

AU - Thomson, Robert C.

AU - Cook, Alonzo D.

AU - Selvig, Knut A.

AU - Hardwick, W. Ross

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N2 - Background: Recombinant human bone morphogenetic protein-2 (rhBMP-2) has been shown to support the regeneration of alveolar bone and periodontal attachment in surgically created periodontal defects and in defects with a history of dental plaque and calculus exposure. Periodontal regeneration has also been shown following guided tissue regeneration using space-providing expanded polytetrafluoroethylene (ePTFE) devices. The objective of this study was to evaluate the influence of rhBMP-2 on regeneration of alveolar bone and periodontal attachment used in conjunction with a space-providing ePTFE device. Methods: Routine, critical-size, 5-6 mm, supra-alveolar, periodontal defects were created around the third and fourth mandibular premolar teeth in four young adult Hound Labrador mongrel dogs. rhBMP-2 (0.2 mg/ml) in an absorbable collagen sponge (rhBMP-2/ACS) or buffer/ACS (control) implants were randomly assigned to be placed around the premolar teeth in the left and right jaw quadrants in subsequent animals. Space-providing ePTFE devices with 300-μm laser-drilled pores, 0.8 mm apart, were used to cover the rhBMP-2 and control implants. The gingival flaps were advanced for primary wound closure. The animals were euthanized at 8 weeks postsurgery for histologic and histometric analyses. Results: Bone regeneration and ankylosis were significantly increased in jaw quadrants receiving rhBMP-2/ACS compared to control (bone height 4.8 ± 0.3 versus 2.0 ± 0.2 mm, p = 0.001; bone area 10.9 ± 1.3 versus 1.4 ± 0.1 mm2; p = 0.009, and ankylosis 2.2 ± 0.2 versus 0.04 ± 0.7 mm; p = 0.01). No differences between groups were found for cementum regeneration and root resorption. Conclusions: rhBMP-2 significantly enhances regeneration of alveolar bone in conjunction with a space-providing, macroporous ePTFE device for GTR.

AB - Background: Recombinant human bone morphogenetic protein-2 (rhBMP-2) has been shown to support the regeneration of alveolar bone and periodontal attachment in surgically created periodontal defects and in defects with a history of dental plaque and calculus exposure. Periodontal regeneration has also been shown following guided tissue regeneration using space-providing expanded polytetrafluoroethylene (ePTFE) devices. The objective of this study was to evaluate the influence of rhBMP-2 on regeneration of alveolar bone and periodontal attachment used in conjunction with a space-providing ePTFE device. Methods: Routine, critical-size, 5-6 mm, supra-alveolar, periodontal defects were created around the third and fourth mandibular premolar teeth in four young adult Hound Labrador mongrel dogs. rhBMP-2 (0.2 mg/ml) in an absorbable collagen sponge (rhBMP-2/ACS) or buffer/ACS (control) implants were randomly assigned to be placed around the premolar teeth in the left and right jaw quadrants in subsequent animals. Space-providing ePTFE devices with 300-μm laser-drilled pores, 0.8 mm apart, were used to cover the rhBMP-2 and control implants. The gingival flaps were advanced for primary wound closure. The animals were euthanized at 8 weeks postsurgery for histologic and histometric analyses. Results: Bone regeneration and ankylosis were significantly increased in jaw quadrants receiving rhBMP-2/ACS compared to control (bone height 4.8 ± 0.3 versus 2.0 ± 0.2 mm, p = 0.001; bone area 10.9 ± 1.3 versus 1.4 ± 0.1 mm2; p = 0.009, and ankylosis 2.2 ± 0.2 versus 0.04 ± 0.7 mm; p = 0.01). No differences between groups were found for cementum regeneration and root resorption. Conclusions: rhBMP-2 significantly enhances regeneration of alveolar bone in conjunction with a space-providing, macroporous ePTFE device for GTR.

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