Peripheral nerve regeneration by the in vitro differentiated-human bone marrow stromal cells with Schwann cell property

Satoshi Shimizu, Masaaki Kitada, Hiroto Ishikawa, Yutaka Itokazu, Shohei Wakao, Mari Dezawa

Research output: Contribution to journalArticle

122 Scopus citations

Abstract

We examined the availability of human bone marrow stromal cells (MSCs) as a source of transplantation therapy in nerve injury. Human MSCs were subjected to a series of treatments with a reducing agent, retinoic acid and a combination of trophic factors. Morphologically and immunocytochemically, such treated cells differentiated into Schwann cell characteristics in vitro. Cells were filled into a transpermeable tube, transplanted into the gap made in the rat sciatic nerve of a rat and followed up to 3 weeks under the control of immunosuppressant. In contrast to untreated human MSCs, differentiated human MSCs expressed Schwann cell markers in vivo and supported regenerating axons. These results suggest that human MSCs can be induced to be a substitute for Schwann cells that may be applied for nerve regeneration.

Original languageEnglish (US)
Pages (from-to)915-920
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume359
Issue number4
DOIs
StatePublished - Aug 10 2007
Externally publishedYes

Keywords

  • Cell therapy
  • Human mesenchymal cells
  • Peripheral nerve injury
  • Reconstruction
  • Schwann cells
  • Transdifferentiation
  • Transplantation

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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