Phagocytic cell activity and periodontitis in Down syndrome

A. Khocht, Barbara Landis Russell, Joseph Gerard Cannon, B. Turner, M. Janal

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: This study investigated the phagocytic function of peripheral granulocytes and monocytes from adult individuals with Down syndrome (DS) and assessed the relation between phagocytic function and periodontal status. Methods: Fifty-five DS individuals (18-56years old), 74 mentally retarded individuals, and 88 medically healthy controls (HC) participated in the study. Gingival inflammation index, plaque index, probing depth, periodontal attachment level (AL), and bleeding on probing were taken for each subject. Whole blood was collected for granulocyte/monocyte phagocytosis tests. Phagocytic function was determined by flow cytometry in terms of percentage of cells actively involved in phagocytosis, and phagocytic intensity (magnitude of the bacterial staining per cell). Results: Phagocytic intensity of both granulocytes and monocytes was comparable in HC and DS subjects. While AL was directly related to phagocytic intensity of both granulocytes (r=0.14, P=0.03) and monocytes (r=0.2, P=0.003) in all subjects, this relationship was stronger in DS than in other subjects, even after controlling for known risk factors for periodontitis (P<0.05). Monocyte phagocytic intensity was the only necessary predictor of AL (P=0.003), indicating a similar relationship between AL and phagocytic activity in either cell type. Conclusions: While granulocyte and monocyte phagocytic intensities are similar in Down and non-DS individuals, phagocytic intensity was associated with more AL in DS than non-DS individuals.

Original languageEnglish (US)
Pages (from-to)346-352
Number of pages7
JournalOral Diseases
Volume18
Issue number4
DOIs
StatePublished - May 1 2012

Fingerprint

Periodontitis
Phagocytes
Down Syndrome
Monocytes
Granulocytes
Phagocytosis
Periodontal Index
Mentally Disabled Persons
Flow Cytometry
Staining and Labeling
Hemorrhage
Inflammation

Keywords

  • Down syndrome
  • Granulocytes
  • Monocytes
  • Periodontitis
  • Phagocytosis

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Dentistry(all)

Cite this

Phagocytic cell activity and periodontitis in Down syndrome. / Khocht, A.; Russell, Barbara Landis; Cannon, Joseph Gerard; Turner, B.; Janal, M.

In: Oral Diseases, Vol. 18, No. 4, 01.05.2012, p. 346-352.

Research output: Contribution to journalArticle

Khocht, A. ; Russell, Barbara Landis ; Cannon, Joseph Gerard ; Turner, B. ; Janal, M. / Phagocytic cell activity and periodontitis in Down syndrome. In: Oral Diseases. 2012 ; Vol. 18, No. 4. pp. 346-352.
@article{6cb2d867606b444b99fb3232a1f89d3f,
title = "Phagocytic cell activity and periodontitis in Down syndrome",
abstract = "Background: This study investigated the phagocytic function of peripheral granulocytes and monocytes from adult individuals with Down syndrome (DS) and assessed the relation between phagocytic function and periodontal status. Methods: Fifty-five DS individuals (18-56years old), 74 mentally retarded individuals, and 88 medically healthy controls (HC) participated in the study. Gingival inflammation index, plaque index, probing depth, periodontal attachment level (AL), and bleeding on probing were taken for each subject. Whole blood was collected for granulocyte/monocyte phagocytosis tests. Phagocytic function was determined by flow cytometry in terms of percentage of cells actively involved in phagocytosis, and phagocytic intensity (magnitude of the bacterial staining per cell). Results: Phagocytic intensity of both granulocytes and monocytes was comparable in HC and DS subjects. While AL was directly related to phagocytic intensity of both granulocytes (r=0.14, P=0.03) and monocytes (r=0.2, P=0.003) in all subjects, this relationship was stronger in DS than in other subjects, even after controlling for known risk factors for periodontitis (P<0.05). Monocyte phagocytic intensity was the only necessary predictor of AL (P=0.003), indicating a similar relationship between AL and phagocytic activity in either cell type. Conclusions: While granulocyte and monocyte phagocytic intensities are similar in Down and non-DS individuals, phagocytic intensity was associated with more AL in DS than non-DS individuals.",
keywords = "Down syndrome, Granulocytes, Monocytes, Periodontitis, Phagocytosis",
author = "A. Khocht and Russell, {Barbara Landis} and Cannon, {Joseph Gerard} and B. Turner and M. Janal",
year = "2012",
month = "5",
day = "1",
doi = "10.1111/j.1601-0825.2011.01877.x",
language = "English (US)",
volume = "18",
pages = "346--352",
journal = "Oral Diseases",
issn = "1354-523X",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - Phagocytic cell activity and periodontitis in Down syndrome

AU - Khocht, A.

AU - Russell, Barbara Landis

AU - Cannon, Joseph Gerard

AU - Turner, B.

AU - Janal, M.

PY - 2012/5/1

Y1 - 2012/5/1

N2 - Background: This study investigated the phagocytic function of peripheral granulocytes and monocytes from adult individuals with Down syndrome (DS) and assessed the relation between phagocytic function and periodontal status. Methods: Fifty-five DS individuals (18-56years old), 74 mentally retarded individuals, and 88 medically healthy controls (HC) participated in the study. Gingival inflammation index, plaque index, probing depth, periodontal attachment level (AL), and bleeding on probing were taken for each subject. Whole blood was collected for granulocyte/monocyte phagocytosis tests. Phagocytic function was determined by flow cytometry in terms of percentage of cells actively involved in phagocytosis, and phagocytic intensity (magnitude of the bacterial staining per cell). Results: Phagocytic intensity of both granulocytes and monocytes was comparable in HC and DS subjects. While AL was directly related to phagocytic intensity of both granulocytes (r=0.14, P=0.03) and monocytes (r=0.2, P=0.003) in all subjects, this relationship was stronger in DS than in other subjects, even after controlling for known risk factors for periodontitis (P<0.05). Monocyte phagocytic intensity was the only necessary predictor of AL (P=0.003), indicating a similar relationship between AL and phagocytic activity in either cell type. Conclusions: While granulocyte and monocyte phagocytic intensities are similar in Down and non-DS individuals, phagocytic intensity was associated with more AL in DS than non-DS individuals.

AB - Background: This study investigated the phagocytic function of peripheral granulocytes and monocytes from adult individuals with Down syndrome (DS) and assessed the relation between phagocytic function and periodontal status. Methods: Fifty-five DS individuals (18-56years old), 74 mentally retarded individuals, and 88 medically healthy controls (HC) participated in the study. Gingival inflammation index, plaque index, probing depth, periodontal attachment level (AL), and bleeding on probing were taken for each subject. Whole blood was collected for granulocyte/monocyte phagocytosis tests. Phagocytic function was determined by flow cytometry in terms of percentage of cells actively involved in phagocytosis, and phagocytic intensity (magnitude of the bacterial staining per cell). Results: Phagocytic intensity of both granulocytes and monocytes was comparable in HC and DS subjects. While AL was directly related to phagocytic intensity of both granulocytes (r=0.14, P=0.03) and monocytes (r=0.2, P=0.003) in all subjects, this relationship was stronger in DS than in other subjects, even after controlling for known risk factors for periodontitis (P<0.05). Monocyte phagocytic intensity was the only necessary predictor of AL (P=0.003), indicating a similar relationship between AL and phagocytic activity in either cell type. Conclusions: While granulocyte and monocyte phagocytic intensities are similar in Down and non-DS individuals, phagocytic intensity was associated with more AL in DS than non-DS individuals.

KW - Down syndrome

KW - Granulocytes

KW - Monocytes

KW - Periodontitis

KW - Phagocytosis

UR - http://www.scopus.com/inward/record.url?scp=84859100867&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84859100867&partnerID=8YFLogxK

U2 - 10.1111/j.1601-0825.2011.01877.x

DO - 10.1111/j.1601-0825.2011.01877.x

M3 - Article

C2 - 22126098

AN - SCOPUS:84859100867

VL - 18

SP - 346

EP - 352

JO - Oral Diseases

JF - Oral Diseases

SN - 1354-523X

IS - 4

ER -