Phagocytosis induces lysosome remodeling and regulated presentation of particulate antigens by activated dendritic cells

Jayakar V. Nayak, David A. Hokey, Adriana Larregina, Yukai He, Russell D. Salter, Simon C. Watkins, Louis D. Falo

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Immunization with particulate Ag effectively induces antitumor and antiviral T cell-mediated immunity. Immature dendritic cells (DCs) efficiently internalize, process, and present a variety of particulate Ags; however, previously published data suggest that both the uptake of soluble Ag through micropinocytosis, and phagocytosis of particulates are significantly curtailed in activated DC populations. In this study, we demonstrate that although macropinocytosis of soluble Ag is diminished following DC activation, subsets of DCs in activated DC populations retain the ability to actively phagocytose particulate Ags. Live cell imaging of activated DCs reveals that phagocytosis of particulates can result in cytoskeletal remodeling and perinuclear lysosome cluster disruption in a time-dependent manner. Interestingly, our results suggest that in activated DC populations, presentation of phagocytosed particulate Ags is dependent on the nature of the activation signal. These results provide direct evidence of functional heterogeneity in DC populations and contribute to the development of particle-based immunization strategies.

Original languageEnglish (US)
Pages (from-to)8493-8503
Number of pages11
JournalJournal of Immunology
Volume177
Issue number12
DOIs
StatePublished - Dec 15 2006

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Antigen Presentation
Lysosomes
Phagocytosis
Dendritic Cells
Population
Immunization
Cytophagocytosis
Cellular Immunity
Antiviral Agents
T-Lymphocytes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Phagocytosis induces lysosome remodeling and regulated presentation of particulate antigens by activated dendritic cells. / Nayak, Jayakar V.; Hokey, David A.; Larregina, Adriana; He, Yukai; Salter, Russell D.; Watkins, Simon C.; Falo, Louis D.

In: Journal of Immunology, Vol. 177, No. 12, 15.12.2006, p. 8493-8503.

Research output: Contribution to journalArticle

Nayak, Jayakar V. ; Hokey, David A. ; Larregina, Adriana ; He, Yukai ; Salter, Russell D. ; Watkins, Simon C. ; Falo, Louis D. / Phagocytosis induces lysosome remodeling and regulated presentation of particulate antigens by activated dendritic cells. In: Journal of Immunology. 2006 ; Vol. 177, No. 12. pp. 8493-8503.
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