Phagocytosis of candida albicans by lymphatic tumour cells in vitro

Mamdooh Ghoneum, Iqbal Grewal, Jimmy J Brown, Ryan Osborne, Hania Elembabi, Gus Gill

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Experiments were carried out to investigate whether different lymphatic tumour cell lines have similar kinetic characteristics of phagocytosis of microorganisms. Six tumour cell lines were used. These were a human T-cell line (CEM), a mouse T-cell line (YAC-1), a human B-cell line (LAZ), and a human erythroleukemic tumour cells (K562), whereas 2 cell lines of professional phagocytosis were used as controls, a human macrophage cell line (THP1) and a mouse macrophage cell line (P388D1). Tumour cells were mixed with candida albicans at a ratio of 10:1 of candida to tumour cells and the percentage of tumour cells that had attached/phagocytosed candida was determined. After 4 h coculture with candida, tumour cells not of T-cell origin (LAZ and K562) showed moderate level of phagocytosis (28%), whereas tumour cells of T-cell origin (CEM and YAC-1) demonstrated low levels of phagocytosis (15%) as compared to macrophage cell lines (THP1 and P388D1) that showed maximum phagocytosis (64-78%). Acid phosphatase (AcPase) activity was increased by 33% during coculture of YAC-1 cells and yeast cells. In conclusion, the results suggest that lymphatic tumour cells of nonphagocytic origin acquire phagocytic properties during the course of malignancy, and digestion of phagocytosed yeast cells maybe related with AcPase activity, as well as that of other lysosomal enzymes. This phenomenon may represent one mechanism by which tumour cells downregulate immune surveillance.

Original languageEnglish (US)
Pages (from-to)127-133
Number of pages7
JournalActa Histochemica
Volume105
Issue number2
DOIs
StatePublished - Jan 1 2003

Fingerprint

Candida albicans
Phagocytosis
Cell Line
Neoplasms
Candida
T-Lymphocytes
Macrophages
Acid Phosphatase
Coculture Techniques
Tumor Cell Line
Yeasts
In Vitro Techniques
Cytophagocytosis
K562 Cells
Digestion
B-Lymphocytes
Down-Regulation
Enzymes

Keywords

  • Candida
  • Lymphatic
  • Phagocytosis
  • Tumour cells

ASJC Scopus subject areas

  • Histology
  • Cell Biology

Cite this

Ghoneum, M., Grewal, I., Brown, J. J., Osborne, R., Elembabi, H., & Gill, G. (2003). Phagocytosis of candida albicans by lymphatic tumour cells in vitro. Acta Histochemica, 105(2), 127-133. https://doi.org/10.1078/0065-1281-00704

Phagocytosis of candida albicans by lymphatic tumour cells in vitro. / Ghoneum, Mamdooh; Grewal, Iqbal; Brown, Jimmy J; Osborne, Ryan; Elembabi, Hania; Gill, Gus.

In: Acta Histochemica, Vol. 105, No. 2, 01.01.2003, p. 127-133.

Research output: Contribution to journalArticle

Ghoneum, M, Grewal, I, Brown, JJ, Osborne, R, Elembabi, H & Gill, G 2003, 'Phagocytosis of candida albicans by lymphatic tumour cells in vitro', Acta Histochemica, vol. 105, no. 2, pp. 127-133. https://doi.org/10.1078/0065-1281-00704
Ghoneum M, Grewal I, Brown JJ, Osborne R, Elembabi H, Gill G. Phagocytosis of candida albicans by lymphatic tumour cells in vitro. Acta Histochemica. 2003 Jan 1;105(2):127-133. https://doi.org/10.1078/0065-1281-00704
Ghoneum, Mamdooh ; Grewal, Iqbal ; Brown, Jimmy J ; Osborne, Ryan ; Elembabi, Hania ; Gill, Gus. / Phagocytosis of candida albicans by lymphatic tumour cells in vitro. In: Acta Histochemica. 2003 ; Vol. 105, No. 2. pp. 127-133.
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