Phenobarbital protection of cerebral NADPH-dependent lipid peroxidation: Possible involvement of a cytosolic glutathione-dependent system

B. Bhattacharyya, T. K. Chatterjee, P. Banerjee, J. J. Ghosh

Research output: Contribution to journalArticle

Abstract

Single (12 h, 24 h, 36 h and 48 h) and repeated (for 10 consecutive days) phenobarbital treatment (75 mg/kg) causes a characteristic alteration in NADPH-induced lipid peroxidation of rat brain tissue. The NADPH-induced lipid peroxidation is significantly inhibited in homogenate as well as in the 18,000 x g supernatant fraction while a significant increase is observed in microsomal preparation after phenobarbital treatment. Cytosolic fraction (105,000 x g supernatant) reversed the microsomal lipid peroxidation and this cytosolic protection is more marked in phenobarbital treated animals. Dialyzing or preheating the cytosol for 10 minutes at 60°C destroys its protective ability. However, glutathione (GSH, 2mM) supplementation of dialyzed cytosol restored this characteristic, while GSH alone or GSH supplemented heated cytosol failed to demonstrate this effect. These results indicate that the stimulation of barbiturate induced protection of cerebral NADPH-induced lipid peroxidation is mediated through a heat labile cytosolic GSH dependent system.

Original languageEnglish (US)
Pages (from-to)52-53
Number of pages2
JournalIRCS Medical Science
Volume12
Issue number1
StatePublished - Jan 1 1984

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Phenobarbital
NADP
Lipid Peroxidation
Glutathione
Cytosol
Lipids
Preheating
Rats
Brain
Animals
Hot Temperature
Tissue

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Phenobarbital protection of cerebral NADPH-dependent lipid peroxidation : Possible involvement of a cytosolic glutathione-dependent system. / Bhattacharyya, B.; Chatterjee, T. K.; Banerjee, P.; Ghosh, J. J.

In: IRCS Medical Science, Vol. 12, No. 1, 01.01.1984, p. 52-53.

Research output: Contribution to journalArticle

Bhattacharyya, B. ; Chatterjee, T. K. ; Banerjee, P. ; Ghosh, J. J. / Phenobarbital protection of cerebral NADPH-dependent lipid peroxidation : Possible involvement of a cytosolic glutathione-dependent system. In: IRCS Medical Science. 1984 ; Vol. 12, No. 1. pp. 52-53.
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