Phenotype and function of murine discrete Peyer's patch macrophage derived - Dendritic cells

Levi H.C. Makala, Julio C.S. Reyes, Yoshifumi Nishikawa, Yoshinori Tsushima, Xuenan Xuan, Xiaohong Huang, Badgar Battsetseg, Tomohide Matsuo, Hideyuki Nagasawa

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


The phenotype and function of peritoneal cavity macrophage-derived dendritic cells (PEC-DC) was previously reported. In this study we have gone further in using our established culture system to generated discrete Peyer's patch dendritic cells (DPP-DC) from murine discrete Peyer's patch macrophages (DPP-Mø), following stimulation with granulocyte macrophage colony stimulating factor (GM-CSF) plus interleukin 4 (IL-4) for 7 days. DPP-Mø from murine small intestines were obtained by mechanical disruption of discrete Peyer's patches (DPP), followed by metrizamide density gradient centrifugation to remove Peyer's patch resident DC and debri, after which an overnight adherent step in tissue culture medium was carried out for macrophage enrichment. Characterization of the generated DPP-DC was carried out using well-established criteria of morphology, expression of membrane antigens and capacity for antigen presentation. Dendritic cells expressed DEC-205, F4/80 and CD34 at high levels, but exhibited very low CD11c levels. They were shown to present soluble protein antigen to CD3+ spleen T cells. A comparison of the surface antigen expression in the progenitor DPP-Mø population and the generated DPP-DC showed a significant decrease in MHC class II levels and a marked down regulation of the co-stimulatory molecule CD86 (B7-2). High expression of the haemopoietic progenitor marker CD34 indicates that the generated DC, possess a haemopoietic rather than myeloid origin. Taken together, these results may provide a better understanding of the complex network regulating mucosal immune responses.

Original languageEnglish (US)
Pages (from-to)491-499
Number of pages9
JournalJournal of Veterinary Medical Science
Issue number4
StatePublished - Apr 2003
Externally publishedYes


  • Discrete Peyer's patch macrophage-derived dendritic cell
  • Growth factor
  • Haemopoietic progenitor
  • Mouse

ASJC Scopus subject areas

  • veterinary(all)


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