Phosphodiesterase 4B (PDE4B) and cAMP-level regulation within different tissue fractions of rat hippocampal slices during long-term potentiation in vitro

T. Ahmed, Julietta Uta Frey

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Molecular events associated with mnemonic processes and neuronal plasticity are postulated to result in functional changes in synaptic structure. One possible site is the post-synaptic density, where activity-dependent changes modulate signal transduction cascades. In this report, we detail spatial-temporal changes for phosphodiesterase 4B (PDE4B) proteins and their substrate cAMP within three neuronal fractions during early and late long-term potentiation (LTP). The cAMP-dependent protein kinase A cascade - which can be regulated by distinct PDE4B activity - is required for mnemonic processes as well as mechanisms of neuronal plasticity, such as those during the maintenance or late-LTP. Fluorescence in situ hybridization studies (FISH) identified no translocation of PDE4B3 from the soma after late-LTP induction indicating a subtle, local control of PDE4B activity. Protein changes were detected within the PSD-enriched fraction. From these results, we conclude that either the changes in PDE4B are due to modulation of pre-existing mRNA, or that the protein is specifically translocated to activated synaptic structures. Furthermore, we report late changes in cAMP levels in the somato-dendritic fraction and discuss this result with the increased PDE4B1/3 doublet in the PSD-enriched fraction.

Original languageEnglish (US)
Pages (from-to)212-222
Number of pages11
JournalBrain Research
Volume1041
Issue number2
DOIs
StatePublished - Apr 18 2005

Fingerprint

Type 4 Cyclic Nucleotide Phosphodiesterase
Long-Term Potentiation
Neuronal Plasticity
Cyclic AMP-Dependent Protein Kinases
Post-Synaptic Density
Proteins
RNA Precursors
Carisoprodol
Fluorescence In Situ Hybridization
Signal Transduction
Maintenance
In Vitro Techniques

Keywords

  • Hippocampus
  • Long-term potentiation
  • Memory formation
  • Neuronal plasticity
  • PDE4
  • Phosphodiesterase
  • cAMP

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Phosphodiesterase 4B (PDE4B) and cAMP-level regulation within different tissue fractions of rat hippocampal slices during long-term potentiation in vitro. / Ahmed, T.; Frey, Julietta Uta.

In: Brain Research, Vol. 1041, No. 2, 18.04.2005, p. 212-222.

Research output: Contribution to journalArticle

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