Phospholipid growth factors and corneal wound wealing

Mitchell Aaron Watsky, May Griffith, De An Wang, Gabor J. Tigyi

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

In many tissue types, wound healing involves cell division and migration over and into the wound area to cover and remodel the wound. LPA and other members of the phospholipid lipid growth factor (PLGF) family stimulate many of the activities involved in wound healing. In the rabbit cornea, we have found that keratocytes from wounded corneas have a volume-activated Cl- current activated by LPA and alkenyl-LPA. This current is minimally activated by cyclic PA and SPC, and is not activated by LPA in cells from uninjured corneas. Biochemical examination of PLGFs in aqueous humor and lacrimal fluid before and after wounding identified LPA, alkenyl-GP, PA, and lyso PS, with elevated PLGF activity after wounding. In recent experiments examining human corneal cell lines and cultured cells using RT-PCR, we found mRNA for EDG receptors 1-5, with an apparent increase in EDG-3, -4, and -5 following brief SDS application to cell lines, and EDG receptors 2-5 induction in late-passage human corneal epithelial cells. This work points to a significant role for PLGFs in the corneal wound-healing process.

Original languageEnglish (US)
Pages (from-to)142-158
Number of pages17
JournalAnnals of the New York Academy of Sciences
Volume905
StatePublished - Jan 1 2000
Externally publishedYes

Fingerprint

Wound Healing
Cornea
Phospholipids
Intercellular Signaling Peptides and Proteins
Cells
Wounds and Injuries
Lipids
Cell Line
Aqueous Humor
Tears
Cell Division
Cell Movement
Cultured Cells
Epithelial Cells
Rabbits
Polymerase Chain Reaction
Messenger RNA
Tissue
Fluids
Experiments

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

Cite this

Phospholipid growth factors and corneal wound wealing. / Watsky, Mitchell Aaron; Griffith, May; Wang, De An; Tigyi, Gabor J.

In: Annals of the New York Academy of Sciences, Vol. 905, 01.01.2000, p. 142-158.

Research output: Contribution to journalArticle

Watsky, MA, Griffith, M, Wang, DA & Tigyi, GJ 2000, 'Phospholipid growth factors and corneal wound wealing', Annals of the New York Academy of Sciences, vol. 905, pp. 142-158.
Watsky, Mitchell Aaron ; Griffith, May ; Wang, De An ; Tigyi, Gabor J. / Phospholipid growth factors and corneal wound wealing. In: Annals of the New York Academy of Sciences. 2000 ; Vol. 905. pp. 142-158.
@article{85929492c6f34e70b05bcc8abec41306,
title = "Phospholipid growth factors and corneal wound wealing",
abstract = "In many tissue types, wound healing involves cell division and migration over and into the wound area to cover and remodel the wound. LPA and other members of the phospholipid lipid growth factor (PLGF) family stimulate many of the activities involved in wound healing. In the rabbit cornea, we have found that keratocytes from wounded corneas have a volume-activated Cl- current activated by LPA and alkenyl-LPA. This current is minimally activated by cyclic PA and SPC, and is not activated by LPA in cells from uninjured corneas. Biochemical examination of PLGFs in aqueous humor and lacrimal fluid before and after wounding identified LPA, alkenyl-GP, PA, and lyso PS, with elevated PLGF activity after wounding. In recent experiments examining human corneal cell lines and cultured cells using RT-PCR, we found mRNA for EDG receptors 1-5, with an apparent increase in EDG-3, -4, and -5 following brief SDS application to cell lines, and EDG receptors 2-5 induction in late-passage human corneal epithelial cells. This work points to a significant role for PLGFs in the corneal wound-healing process.",
author = "Watsky, {Mitchell Aaron} and May Griffith and Wang, {De An} and Tigyi, {Gabor J.}",
year = "2000",
month = "1",
day = "1",
language = "English (US)",
volume = "905",
pages = "142--158",
journal = "Annals of the New York Academy of Sciences",
issn = "0077-8923",
publisher = "Wiley-Blackwell",

}

TY - JOUR

T1 - Phospholipid growth factors and corneal wound wealing

AU - Watsky, Mitchell Aaron

AU - Griffith, May

AU - Wang, De An

AU - Tigyi, Gabor J.

PY - 2000/1/1

Y1 - 2000/1/1

N2 - In many tissue types, wound healing involves cell division and migration over and into the wound area to cover and remodel the wound. LPA and other members of the phospholipid lipid growth factor (PLGF) family stimulate many of the activities involved in wound healing. In the rabbit cornea, we have found that keratocytes from wounded corneas have a volume-activated Cl- current activated by LPA and alkenyl-LPA. This current is minimally activated by cyclic PA and SPC, and is not activated by LPA in cells from uninjured corneas. Biochemical examination of PLGFs in aqueous humor and lacrimal fluid before and after wounding identified LPA, alkenyl-GP, PA, and lyso PS, with elevated PLGF activity after wounding. In recent experiments examining human corneal cell lines and cultured cells using RT-PCR, we found mRNA for EDG receptors 1-5, with an apparent increase in EDG-3, -4, and -5 following brief SDS application to cell lines, and EDG receptors 2-5 induction in late-passage human corneal epithelial cells. This work points to a significant role for PLGFs in the corneal wound-healing process.

AB - In many tissue types, wound healing involves cell division and migration over and into the wound area to cover and remodel the wound. LPA and other members of the phospholipid lipid growth factor (PLGF) family stimulate many of the activities involved in wound healing. In the rabbit cornea, we have found that keratocytes from wounded corneas have a volume-activated Cl- current activated by LPA and alkenyl-LPA. This current is minimally activated by cyclic PA and SPC, and is not activated by LPA in cells from uninjured corneas. Biochemical examination of PLGFs in aqueous humor and lacrimal fluid before and after wounding identified LPA, alkenyl-GP, PA, and lyso PS, with elevated PLGF activity after wounding. In recent experiments examining human corneal cell lines and cultured cells using RT-PCR, we found mRNA for EDG receptors 1-5, with an apparent increase in EDG-3, -4, and -5 following brief SDS application to cell lines, and EDG receptors 2-5 induction in late-passage human corneal epithelial cells. This work points to a significant role for PLGFs in the corneal wound-healing process.

UR - http://www.scopus.com/inward/record.url?scp=0342693056&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0342693056&partnerID=8YFLogxK

M3 - Article

VL - 905

SP - 142

EP - 158

JO - Annals of the New York Academy of Sciences

JF - Annals of the New York Academy of Sciences

SN - 0077-8923

ER -