Phosphorylation of Nuclear Proteins in Myelinating Oligodendrocytes and Its Control by Cyclic AMP

Carmen Sato‐Bigbee, Robert K Yu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

: Oligodendroglial nuclei isolated from rat brains at different stages of myelinogenesis (10,18, and 30 days of age) were incubated with (γ‐32P]ATP and extracted with 0.75 Mperchloric acid to yield a fraction of nonacidic chromatin proteins. The protein extracts were then analyzed by poly‐acrylamide gel electrophoresis. The phosphorylation pattern of these proteins was found to be different for different age groups. In 10‐day‐old rat Oligodendrocytes the most extensive phosphorylation occurred in low molecular mass species (<30 kDa), in contrast to fractions obtained from 18‐and 30‐day‐old rat Oligodendrocytes which showed a significantly higher labeling of the proteins with molecular masses >30 kDa. The phosphorylation of the latter species was greatly stimulated by the presence of cyclic AMP in the incubation media. The results suggest that the phosphorylation of specific nuclear proteins, which may play a regulatory role at different stages of oligodendroglial maturation and myelinogenesis, may be at least partially modulated by intracellular cyclic AMP.

Original languageEnglish (US)
Pages (from-to)1650-1655
Number of pages6
JournalJournal of Neurochemistry
Volume57
Issue number5
DOIs
StatePublished - Jan 1 1991
Externally publishedYes

Fingerprint

Phosphorylation
Oligodendroglia
Nuclear Proteins
Cyclic AMP
Rats
Proteins
Molecular mass
Electrophoresis
Chromatin
Polyacrylamide Gel Electrophoresis
Brain
Age Groups
Adenosine Triphosphate
Acids

Keywords

  • Myelination
  • Nuclear protein phosphorylation
  • Oligodendrocytes

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Phosphorylation of Nuclear Proteins in Myelinating Oligodendrocytes and Its Control by Cyclic AMP. / Sato‐Bigbee, Carmen; Yu, Robert K.

In: Journal of Neurochemistry, Vol. 57, No. 5, 01.01.1991, p. 1650-1655.

Research output: Contribution to journalArticle

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N2 - : Oligodendroglial nuclei isolated from rat brains at different stages of myelinogenesis (10,18, and 30 days of age) were incubated with (γ‐32P]ATP and extracted with 0.75 Mperchloric acid to yield a fraction of nonacidic chromatin proteins. The protein extracts were then analyzed by poly‐acrylamide gel electrophoresis. The phosphorylation pattern of these proteins was found to be different for different age groups. In 10‐day‐old rat Oligodendrocytes the most extensive phosphorylation occurred in low molecular mass species (<30 kDa), in contrast to fractions obtained from 18‐and 30‐day‐old rat Oligodendrocytes which showed a significantly higher labeling of the proteins with molecular masses >30 kDa. The phosphorylation of the latter species was greatly stimulated by the presence of cyclic AMP in the incubation media. The results suggest that the phosphorylation of specific nuclear proteins, which may play a regulatory role at different stages of oligodendroglial maturation and myelinogenesis, may be at least partially modulated by intracellular cyclic AMP.

AB - : Oligodendroglial nuclei isolated from rat brains at different stages of myelinogenesis (10,18, and 30 days of age) were incubated with (γ‐32P]ATP and extracted with 0.75 Mperchloric acid to yield a fraction of nonacidic chromatin proteins. The protein extracts were then analyzed by poly‐acrylamide gel electrophoresis. The phosphorylation pattern of these proteins was found to be different for different age groups. In 10‐day‐old rat Oligodendrocytes the most extensive phosphorylation occurred in low molecular mass species (<30 kDa), in contrast to fractions obtained from 18‐and 30‐day‐old rat Oligodendrocytes which showed a significantly higher labeling of the proteins with molecular masses >30 kDa. The phosphorylation of the latter species was greatly stimulated by the presence of cyclic AMP in the incubation media. The results suggest that the phosphorylation of specific nuclear proteins, which may play a regulatory role at different stages of oligodendroglial maturation and myelinogenesis, may be at least partially modulated by intracellular cyclic AMP.

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