Phosphorylation of the C-terminal domain of RNA polymerase II by the extracellular-signal-regulated protein kinase ERK2

Rhea-Beth Markowitz, April S. Hermann, Daniel F. Taylor, Li Yan He, Spencer Anthony-Cahill, Natalie G. Ahn, William S. Dynan

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Rat ERK2, an extracellular-signal-regulated protein kinase family member, phosphorylates RNA polymerase II in vitro. Phosphorylation occurs within the heptapeptide repeats of the C-terminal domain of the largest subunit, in a region important for regulation of transcriptional activity. Analysis of deletion mutants and synthetic peptides showed that ERK2 phosphorylation occurrs at multiple serine residues throughout the C-terminal domain, with no marked preference for consensus repeats versus naturally occurring variants. Our results are consistent with the idea that protein kinases in the extracellular-signal-regulated protein kinase family regulate transcription by direct phosphorylation of RNA polymerase II, but do not support a model where particular portions of the C-terminal domain are special targets of ERK phosphorylation.

Original languageEnglish (US)
Pages (from-to)1051-1057
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume207
Issue number3
DOIs
StatePublished - Feb 27 1995
Externally publishedYes

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Phosphorylation
RNA Polymerase II
Extracellular Signal-Regulated MAP Kinases
Protein Kinases
Terminal Repeat Sequences
Transcription
Serine
Rats
Consensus
Peptides

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Phosphorylation of the C-terminal domain of RNA polymerase II by the extracellular-signal-regulated protein kinase ERK2. / Markowitz, Rhea-Beth; Hermann, April S.; Taylor, Daniel F.; He, Li Yan; Anthony-Cahill, Spencer; Ahn, Natalie G.; Dynan, William S.

In: Biochemical and Biophysical Research Communications, Vol. 207, No. 3, 27.02.1995, p. 1051-1057.

Research output: Contribution to journalArticle

Markowitz, Rhea-Beth ; Hermann, April S. ; Taylor, Daniel F. ; He, Li Yan ; Anthony-Cahill, Spencer ; Ahn, Natalie G. ; Dynan, William S. / Phosphorylation of the C-terminal domain of RNA polymerase II by the extracellular-signal-regulated protein kinase ERK2. In: Biochemical and Biophysical Research Communications. 1995 ; Vol. 207, No. 3. pp. 1051-1057.
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