Phosphotyrosine signaling analysis in human tumors is confounded by systemic ischemia-driven artifacts and intra-specimen heterogeneity

Aaron S. Gajadhar, Hannah Johnson, Robbert J.C. Slebos, Kent Shaddox, Kerry Wiles, Mary Kay Washington, Alan Joseph Herline, Douglas A. Levine, Daniel C. Liebler, Forest M. White

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Tumor protein phosphorylation analysis may provide insight into intracellular signaling networks underlying tumor behavior, revealing diagnostic, prognostic or therapeutic information. Human tumors collected by The Cancer Genome Atlas program potentially offer the opportunity to characterize activated networks driving tumor progression, in parallel with the genetic and transcriptional landscape already documented for these tumors. However, a critical question is whether cellular signaling networks can be reliably analyzed in surgical specimens, where freezing delays and spatial sampling disparities may potentially obscure physiologic signaling. To quantify the extent of these effects, we analyzed the stability of phosphotyrosine (pTyr) sites in ovarian and colon tumors collected under conditions of controlled ischemia and in the context of defined intratumoral sampling. Cold-ischemia produced a rapid, unpredictable, and widespread impact on tumor pTyr networks within 5 minutes of resection, altering up to 50% of pTyr sites by more than 2-fold. Effects on adhesion and migration, inflammatory response, proliferation, and stress response pathways were recapitulated in both ovarian and colon tumors. In addition, sampling of spatially distinct colon tumor biopsies revealed pTyr differences as dramatic as those associated with ischemic times, despite uniform protein expression profiles. Moreover, intratumoral spatial heterogeneity and pTyr dynamic response to ischemia varied dramatically between tumors collected from different patients. Overall, these findings reveal unforeseen phosphorylation complexity, thereby increasing the difficulty of extracting physiologically relevant pTyr signaling networks from archived tissue specimens. In light of this data, prospective tumor pTyr analysis will require appropriate sampling and collection protocols to preserve in vivo signaling features.

Original languageEnglish (US)
Pages (from-to)1495-1503
Number of pages9
JournalCancer Research
Volume75
Issue number7
DOIs
StatePublished - Apr 1 2015

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Phosphotyrosine
Artifacts
Ischemia
Neoplasms
Colon
Phosphorylation
Cold Ischemia
Atlases
Freezing
Proteins

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Gajadhar, A. S., Johnson, H., Slebos, R. J. C., Shaddox, K., Wiles, K., Washington, M. K., ... White, F. M. (2015). Phosphotyrosine signaling analysis in human tumors is confounded by systemic ischemia-driven artifacts and intra-specimen heterogeneity. Cancer Research, 75(7), 1495-1503. https://doi.org/10.1158/0008-5472.CAN-14-2309

Phosphotyrosine signaling analysis in human tumors is confounded by systemic ischemia-driven artifacts and intra-specimen heterogeneity. / Gajadhar, Aaron S.; Johnson, Hannah; Slebos, Robbert J.C.; Shaddox, Kent; Wiles, Kerry; Washington, Mary Kay; Herline, Alan Joseph; Levine, Douglas A.; Liebler, Daniel C.; White, Forest M.

In: Cancer Research, Vol. 75, No. 7, 01.04.2015, p. 1495-1503.

Research output: Contribution to journalArticle

Gajadhar, AS, Johnson, H, Slebos, RJC, Shaddox, K, Wiles, K, Washington, MK, Herline, AJ, Levine, DA, Liebler, DC & White, FM 2015, 'Phosphotyrosine signaling analysis in human tumors is confounded by systemic ischemia-driven artifacts and intra-specimen heterogeneity', Cancer Research, vol. 75, no. 7, pp. 1495-1503. https://doi.org/10.1158/0008-5472.CAN-14-2309
Gajadhar, Aaron S. ; Johnson, Hannah ; Slebos, Robbert J.C. ; Shaddox, Kent ; Wiles, Kerry ; Washington, Mary Kay ; Herline, Alan Joseph ; Levine, Douglas A. ; Liebler, Daniel C. ; White, Forest M. / Phosphotyrosine signaling analysis in human tumors is confounded by systemic ischemia-driven artifacts and intra-specimen heterogeneity. In: Cancer Research. 2015 ; Vol. 75, No. 7. pp. 1495-1503.
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