Photobiomodulation on bax and Bcl-2 proteins and SIRT1/PGC-1 α axis mRNA expression levels of aging rat skeletal muscle

Fang Hui Li, Yan Ying Liu, Fei Qin, Qing Luo, Hai Ping Yang, Quan Guang Zhang, Timon Cheng Yi Liu

Research output: Contribution to journalArticle

Abstract

Objective. This study aimed to analyze the effects of low level laser irradiation (LLLI) on Bax and IGF-1 and Bcl-2 protein contents and SIRT1/PGC-1α axis mRNA expression levels to prevent sarcopenia in aged rats. Material and Methods. Twenty female Sprague Dawley rats (18 months old) were randomly divided into two groups (n = 10 per group): control (CON) and LLLI groups. The gallium-aluminum-arsenium (GaAlAs) laser irradiation at 810 nm was used in the single point contact mode (3.75 J/cm2; 0.4 cm 2; 125 mW/cm2; 30 s). Bax, Bcl-2, and IGF-1 proteins and SIRT1/PGC-1α axis mRNA expression were assessed 24 h after LLLI on gastrocnemius in aged rat. Results. Gastrocnemius muscle weights, gastrocnemius mass/body mass, Bcl-2/BAX ratio, Bcl-2 protein, IGF-1 protein, and the mRNA contents in SIRT1, PGC-1α, NRF1, TMF, and SOD2 were significantly (P < 0.05) increased by LLLI compared to CON group without LLLI. However, levels of BAX protein and caspase 3 mRNA were significantly attenuated by LLLI compared to CON group (P < 0.05). Conclusion. LLLI at 810 nm inhibits sarcopenia associated with upregulation of Bcl-2/BAX ratio and IGF-1 and SIRT1/PGC-1α axis mRNA expression in aged rats. This indicates that LLLI has potential to decrease progression of myocyte apoptosis in sarcopenic muscles.

Original languageEnglish (US)
Article number384816
JournalInternational Journal of Photoenergy
Volume2014
DOIs
StatePublished - 2014

Fingerprint

skeletal muscle
Laser beam effects
rats
Muscle
Rats
Aging of materials
proteins
Proteins
Messenger RNA
irradiation
Insulin-Like Growth Factor I
lasers
muscles
weight (mass)
Gallium
muscle cells
Point contacts
Cell death
Aluminum
apoptosis

ASJC Scopus subject areas

  • Chemistry(all)
  • Atomic and Molecular Physics, and Optics
  • Renewable Energy, Sustainability and the Environment
  • Materials Science(all)

Cite this

Photobiomodulation on bax and Bcl-2 proteins and SIRT1/PGC-1 α axis mRNA expression levels of aging rat skeletal muscle. / Li, Fang Hui; Liu, Yan Ying; Qin, Fei; Luo, Qing; Yang, Hai Ping; Zhang, Quan Guang; Liu, Timon Cheng Yi.

In: International Journal of Photoenergy, Vol. 2014, 384816, 2014.

Research output: Contribution to journalArticle

Li, Fang Hui ; Liu, Yan Ying ; Qin, Fei ; Luo, Qing ; Yang, Hai Ping ; Zhang, Quan Guang ; Liu, Timon Cheng Yi. / Photobiomodulation on bax and Bcl-2 proteins and SIRT1/PGC-1 α axis mRNA expression levels of aging rat skeletal muscle. In: International Journal of Photoenergy. 2014 ; Vol. 2014.
@article{98a89b2f4af94265a1e1cd6fbab43f78,
title = "Photobiomodulation on bax and Bcl-2 proteins and SIRT1/PGC-1 α axis mRNA expression levels of aging rat skeletal muscle",
abstract = "Objective. This study aimed to analyze the effects of low level laser irradiation (LLLI) on Bax and IGF-1 and Bcl-2 protein contents and SIRT1/PGC-1α axis mRNA expression levels to prevent sarcopenia in aged rats. Material and Methods. Twenty female Sprague Dawley rats (18 months old) were randomly divided into two groups (n = 10 per group): control (CON) and LLLI groups. The gallium-aluminum-arsenium (GaAlAs) laser irradiation at 810 nm was used in the single point contact mode (3.75 J/cm2; 0.4 cm 2; 125 mW/cm2; 30 s). Bax, Bcl-2, and IGF-1 proteins and SIRT1/PGC-1α axis mRNA expression were assessed 24 h after LLLI on gastrocnemius in aged rat. Results. Gastrocnemius muscle weights, gastrocnemius mass/body mass, Bcl-2/BAX ratio, Bcl-2 protein, IGF-1 protein, and the mRNA contents in SIRT1, PGC-1α, NRF1, TMF, and SOD2 were significantly (P < 0.05) increased by LLLI compared to CON group without LLLI. However, levels of BAX protein and caspase 3 mRNA were significantly attenuated by LLLI compared to CON group (P < 0.05). Conclusion. LLLI at 810 nm inhibits sarcopenia associated with upregulation of Bcl-2/BAX ratio and IGF-1 and SIRT1/PGC-1α axis mRNA expression in aged rats. This indicates that LLLI has potential to decrease progression of myocyte apoptosis in sarcopenic muscles.",
author = "Li, {Fang Hui} and Liu, {Yan Ying} and Fei Qin and Qing Luo and Yang, {Hai Ping} and Zhang, {Quan Guang} and Liu, {Timon Cheng Yi}",
year = "2014",
doi = "10.1155/2014/384816",
language = "English (US)",
volume = "2014",
journal = "International Journal of Photoenergy",
issn = "1110-662X",
publisher = "Hindawi Publishing Corporation",

}

TY - JOUR

T1 - Photobiomodulation on bax and Bcl-2 proteins and SIRT1/PGC-1 α axis mRNA expression levels of aging rat skeletal muscle

AU - Li, Fang Hui

AU - Liu, Yan Ying

AU - Qin, Fei

AU - Luo, Qing

AU - Yang, Hai Ping

AU - Zhang, Quan Guang

AU - Liu, Timon Cheng Yi

PY - 2014

Y1 - 2014

N2 - Objective. This study aimed to analyze the effects of low level laser irradiation (LLLI) on Bax and IGF-1 and Bcl-2 protein contents and SIRT1/PGC-1α axis mRNA expression levels to prevent sarcopenia in aged rats. Material and Methods. Twenty female Sprague Dawley rats (18 months old) were randomly divided into two groups (n = 10 per group): control (CON) and LLLI groups. The gallium-aluminum-arsenium (GaAlAs) laser irradiation at 810 nm was used in the single point contact mode (3.75 J/cm2; 0.4 cm 2; 125 mW/cm2; 30 s). Bax, Bcl-2, and IGF-1 proteins and SIRT1/PGC-1α axis mRNA expression were assessed 24 h after LLLI on gastrocnemius in aged rat. Results. Gastrocnemius muscle weights, gastrocnemius mass/body mass, Bcl-2/BAX ratio, Bcl-2 protein, IGF-1 protein, and the mRNA contents in SIRT1, PGC-1α, NRF1, TMF, and SOD2 were significantly (P < 0.05) increased by LLLI compared to CON group without LLLI. However, levels of BAX protein and caspase 3 mRNA were significantly attenuated by LLLI compared to CON group (P < 0.05). Conclusion. LLLI at 810 nm inhibits sarcopenia associated with upregulation of Bcl-2/BAX ratio and IGF-1 and SIRT1/PGC-1α axis mRNA expression in aged rats. This indicates that LLLI has potential to decrease progression of myocyte apoptosis in sarcopenic muscles.

AB - Objective. This study aimed to analyze the effects of low level laser irradiation (LLLI) on Bax and IGF-1 and Bcl-2 protein contents and SIRT1/PGC-1α axis mRNA expression levels to prevent sarcopenia in aged rats. Material and Methods. Twenty female Sprague Dawley rats (18 months old) were randomly divided into two groups (n = 10 per group): control (CON) and LLLI groups. The gallium-aluminum-arsenium (GaAlAs) laser irradiation at 810 nm was used in the single point contact mode (3.75 J/cm2; 0.4 cm 2; 125 mW/cm2; 30 s). Bax, Bcl-2, and IGF-1 proteins and SIRT1/PGC-1α axis mRNA expression were assessed 24 h after LLLI on gastrocnemius in aged rat. Results. Gastrocnemius muscle weights, gastrocnemius mass/body mass, Bcl-2/BAX ratio, Bcl-2 protein, IGF-1 protein, and the mRNA contents in SIRT1, PGC-1α, NRF1, TMF, and SOD2 were significantly (P < 0.05) increased by LLLI compared to CON group without LLLI. However, levels of BAX protein and caspase 3 mRNA were significantly attenuated by LLLI compared to CON group (P < 0.05). Conclusion. LLLI at 810 nm inhibits sarcopenia associated with upregulation of Bcl-2/BAX ratio and IGF-1 and SIRT1/PGC-1α axis mRNA expression in aged rats. This indicates that LLLI has potential to decrease progression of myocyte apoptosis in sarcopenic muscles.

UR - http://www.scopus.com/inward/record.url?scp=84904096664&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84904096664&partnerID=8YFLogxK

U2 - 10.1155/2014/384816

DO - 10.1155/2014/384816

M3 - Article

AN - SCOPUS:84904096664

VL - 2014

JO - International Journal of Photoenergy

JF - International Journal of Photoenergy

SN - 1110-662X

M1 - 384816

ER -