Photobiomodulation preconditioning prevents cognitive impairment in a neonatal rat model of hypoxia-ischemia

Luodan Yang, Yan Dong, Chongyun Wu, Yong Li, Yichen Guo, Baocheng Yang, Xuemei Zong, Michael R. Hamblin, Timon C.Y. Liu, Quanguang Zhang

Research output: Contribution to journalArticle

Abstract

Neonatal hypoxia-ischemia (HI) injury caused by oxygen deprivation is the most common cause of mortality and severe neurologic deficits in neonates. The present work evaluated the preventative effect of photobiomodulation (PBM) preconditioning, and its underlying mechanism of action on brain damage in an HI model in neonatal rats. According to the optimal time response of ATP levels in brain samples removed from normal rats, a PBM preconditioning (PBM-P) regimen (808 nm CW laser, 1 cm2 spot, 100 mW/cm2, 12 J/cm2) was delivered to the scalp 6 hours before HI. PBM-P significantly attenuated cognitive impairment, volume shrinkage in the brain, neuron loss, dendritic and synaptic injury after HI. Further mechanistic investigation found that PBM-P could restore HI-induced mitochondrial dynamics and inhibit mitochondrial fragmentation, followed by a robust suppression of cytochrome c release, and prevention of neuronal apoptosis by inhibition of caspase activation. Our work suggests that PBM-P can attenuate HI-induced brain injury by maintaining mitochondrial dynamics and inhibiting the mitochondrial apoptotic pathway.

Original languageEnglish (US)
Article numbere201800359
JournalJournal of Biophotonics
Volume12
Issue number6
DOIs
StatePublished - Jun 1 2019

Fingerprint

ischemia
preconditioning
hypoxia
impairment
rats
Rats
Brain
Ischemia
brain damage
Mitochondrial Dynamics
brain
Continuous wave lasers
Adenosinetriphosphate
Cell death
Caspases
Cytochromes c
deprivation
Neurons
adenosine triphosphate
Adenosine Triphosphate

Keywords

  • cognitive impairment
  • hypoxia-ischemia
  • mitochondria
  • neuroprotection
  • photobiomodulation preconditioning

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Photobiomodulation preconditioning prevents cognitive impairment in a neonatal rat model of hypoxia-ischemia. / Yang, Luodan; Dong, Yan; Wu, Chongyun; Li, Yong; Guo, Yichen; Yang, Baocheng; Zong, Xuemei; Hamblin, Michael R.; Liu, Timon C.Y.; Zhang, Quanguang.

In: Journal of Biophotonics, Vol. 12, No. 6, e201800359, 01.06.2019.

Research output: Contribution to journalArticle

Yang, L, Dong, Y, Wu, C, Li, Y, Guo, Y, Yang, B, Zong, X, Hamblin, MR, Liu, TCY & Zhang, Q 2019, 'Photobiomodulation preconditioning prevents cognitive impairment in a neonatal rat model of hypoxia-ischemia', Journal of Biophotonics, vol. 12, no. 6, e201800359. https://doi.org/10.1002/jbio.201800359
Yang, Luodan ; Dong, Yan ; Wu, Chongyun ; Li, Yong ; Guo, Yichen ; Yang, Baocheng ; Zong, Xuemei ; Hamblin, Michael R. ; Liu, Timon C.Y. ; Zhang, Quanguang. / Photobiomodulation preconditioning prevents cognitive impairment in a neonatal rat model of hypoxia-ischemia. In: Journal of Biophotonics. 2019 ; Vol. 12, No. 6.
@article{b0cc881bfc0c4f81a97624df2d838530,
title = "Photobiomodulation preconditioning prevents cognitive impairment in a neonatal rat model of hypoxia-ischemia",
abstract = "Neonatal hypoxia-ischemia (HI) injury caused by oxygen deprivation is the most common cause of mortality and severe neurologic deficits in neonates. The present work evaluated the preventative effect of photobiomodulation (PBM) preconditioning, and its underlying mechanism of action on brain damage in an HI model in neonatal rats. According to the optimal time response of ATP levels in brain samples removed from normal rats, a PBM preconditioning (PBM-P) regimen (808 nm CW laser, 1 cm2 spot, 100 mW/cm2, 12 J/cm2) was delivered to the scalp 6 hours before HI. PBM-P significantly attenuated cognitive impairment, volume shrinkage in the brain, neuron loss, dendritic and synaptic injury after HI. Further mechanistic investigation found that PBM-P could restore HI-induced mitochondrial dynamics and inhibit mitochondrial fragmentation, followed by a robust suppression of cytochrome c release, and prevention of neuronal apoptosis by inhibition of caspase activation. Our work suggests that PBM-P can attenuate HI-induced brain injury by maintaining mitochondrial dynamics and inhibiting the mitochondrial apoptotic pathway.",
keywords = "cognitive impairment, hypoxia-ischemia, mitochondria, neuroprotection, photobiomodulation preconditioning",
author = "Luodan Yang and Yan Dong and Chongyun Wu and Yong Li and Yichen Guo and Baocheng Yang and Xuemei Zong and Hamblin, {Michael R.} and Liu, {Timon C.Y.} and Quanguang Zhang",
year = "2019",
month = "6",
day = "1",
doi = "10.1002/jbio.201800359",
language = "English (US)",
volume = "12",
journal = "Journal of Biophotonics",
issn = "1864-063X",
publisher = "Wiley-VCH Verlag",
number = "6",

}

TY - JOUR

T1 - Photobiomodulation preconditioning prevents cognitive impairment in a neonatal rat model of hypoxia-ischemia

AU - Yang, Luodan

AU - Dong, Yan

AU - Wu, Chongyun

AU - Li, Yong

AU - Guo, Yichen

AU - Yang, Baocheng

AU - Zong, Xuemei

AU - Hamblin, Michael R.

AU - Liu, Timon C.Y.

AU - Zhang, Quanguang

PY - 2019/6/1

Y1 - 2019/6/1

N2 - Neonatal hypoxia-ischemia (HI) injury caused by oxygen deprivation is the most common cause of mortality and severe neurologic deficits in neonates. The present work evaluated the preventative effect of photobiomodulation (PBM) preconditioning, and its underlying mechanism of action on brain damage in an HI model in neonatal rats. According to the optimal time response of ATP levels in brain samples removed from normal rats, a PBM preconditioning (PBM-P) regimen (808 nm CW laser, 1 cm2 spot, 100 mW/cm2, 12 J/cm2) was delivered to the scalp 6 hours before HI. PBM-P significantly attenuated cognitive impairment, volume shrinkage in the brain, neuron loss, dendritic and synaptic injury after HI. Further mechanistic investigation found that PBM-P could restore HI-induced mitochondrial dynamics and inhibit mitochondrial fragmentation, followed by a robust suppression of cytochrome c release, and prevention of neuronal apoptosis by inhibition of caspase activation. Our work suggests that PBM-P can attenuate HI-induced brain injury by maintaining mitochondrial dynamics and inhibiting the mitochondrial apoptotic pathway.

AB - Neonatal hypoxia-ischemia (HI) injury caused by oxygen deprivation is the most common cause of mortality and severe neurologic deficits in neonates. The present work evaluated the preventative effect of photobiomodulation (PBM) preconditioning, and its underlying mechanism of action on brain damage in an HI model in neonatal rats. According to the optimal time response of ATP levels in brain samples removed from normal rats, a PBM preconditioning (PBM-P) regimen (808 nm CW laser, 1 cm2 spot, 100 mW/cm2, 12 J/cm2) was delivered to the scalp 6 hours before HI. PBM-P significantly attenuated cognitive impairment, volume shrinkage in the brain, neuron loss, dendritic and synaptic injury after HI. Further mechanistic investigation found that PBM-P could restore HI-induced mitochondrial dynamics and inhibit mitochondrial fragmentation, followed by a robust suppression of cytochrome c release, and prevention of neuronal apoptosis by inhibition of caspase activation. Our work suggests that PBM-P can attenuate HI-induced brain injury by maintaining mitochondrial dynamics and inhibiting the mitochondrial apoptotic pathway.

KW - cognitive impairment

KW - hypoxia-ischemia

KW - mitochondria

KW - neuroprotection

KW - photobiomodulation preconditioning

UR - http://www.scopus.com/inward/record.url?scp=85061614274&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85061614274&partnerID=8YFLogxK

U2 - 10.1002/jbio.201800359

DO - 10.1002/jbio.201800359

M3 - Article

VL - 12

JO - Journal of Biophotonics

JF - Journal of Biophotonics

SN - 1864-063X

IS - 6

M1 - e201800359

ER -