Phylogenetic diversity of Mycobacterium tuberculosis in two geographically distinct locations in Botswana – The Kopanyo Study

Eleanor S. Click, Alyssa Finlay, John E. Oeltmann, Joyce Basotli, Chawangwa Modongo, Rosanna Boyd, Xiao Jun Wen, James Shepherd, Patrick K. Moonan, Nicola M. Zetola

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Mycobacterium tuberculosis complex (MTBC) is divided into several major phylogenetic lineages, with differential distribution globally. Using population-based data collected over a three year period, we performed 24-locus Mycobacterial Interspersed Repeat Unit – Variable Number Tandem Repeat (MIRU-VNTR) genotyping on all culture isolates from two districts of the country that differ in tuberculosis (TB) incidence (Gaborone, the capital, and Ghanzi in the Western Kalahari). The study objective was to characterize the molecular epidemiology of TB in these districts. Overall phylogenetic diversity mirrored that reported from neighboring Republic of South Africa, but differences in the two districts were marked. All four major lineages of M. tuberculosis were found in Gaborone, but only three of the four major lineages were found in Ghanzi. Strain diversity was lower in Ghanzi, with a large proportion (38%) of all isolates having an identical MIRU-VNTR result, compared to 6% of all isolates in Gaborone with the same MIRU-VNTR result. This study demonstrates localized differences in strain diversity by two districts in Botswana, and contributes to a growing characterization of MTBC diversity globally.

Original languageEnglish (US)
Article number104232
JournalInfection, Genetics and Evolution
Volume81
DOIs
StatePublished - Jul 2020
Externally publishedYes

Keywords

  • Botswana
  • Diversity
  • Lineage
  • Molecular epidemiology
  • Tuberculosis

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Microbiology (medical)
  • Infectious Diseases

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