Physical mobilization of secretory vesicles facilitates neuropeptide release by nerve growth factor-differentiated PC12 cells

Yuen Keng Ng, Xinghua Lu, Edwin S. Levitan

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

It has been speculated that neurosecretion can be enhanced by increasing the motion, and hence, the availability of cytoplasmic secretory vesicles. However, facilitator-induced physical mobilization of secretory vesicles has not been observed directly in living cells, and recent experimental results call this hypothesis into question. Here, high resolution green fluorescent protein (GFP)-based measurements in nerve growth factor-differentiated PC12 cells are used to test whether altering dense core vesicle (DCV) motion affects neuropeptide release. Experiments with mycalolide B and jasplakinolide demonstrate that neuropeptidergic DCV motion at the ends of processes is proportional to F-actin. Furthermore, Ba2+ increases DCV mobility without detectably modifying F-actin. Finally, we show that altering DCV motion by changing F-actin or stimulating with Ba2+ proportionally changes sustained neuropeptide release. Therefore, increasing DCV mobility facilitates prolonged neuropeptide release.

Original languageEnglish (US)
Pages (from-to)395-402
Number of pages8
JournalJournal of Physiology
Volume542
Issue number2
DOIs
StatePublished - Jul 15 2002
Externally publishedYes

ASJC Scopus subject areas

  • Physiology

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