Physicochemical characteristics of interaction of toxic triterpene glycosides from holothurians with rat brain Na+-K+-ATPase

I. A. Gorshkova, A. I. Kalinovsky, S. G. Ilyin, B. A. Gorshkov, V. A. Stonik

Research output: Contribution to journalArticle

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Abstract

I. A. Gorshkova, A. I. Kalinovsky, S. G. Ilyin, B. A. Gorshkov and V. A. Stonik. Physicochemical characteristics of interaction of toxic triterpene glycosides from holothurians with rat brain Na+-K+-ATPase. Toxicon 27, 937-945, 1989.-High-angle X-ray diffraction spectra showed that triterpene glycosides form crystalline complexes with membrane cholesterol. Electron microscopy demonstrated a decreased vesicle size, of the membrane preparation from rat brain which is enriched in Na+-K+-ATPase, by the triterpene glycosides. The Arrhenius plot was linear in the presence of triterpene glycosides. The half-width of the phosphatidylcholine N-methyl proton line in proton NMR spectra was not altered in the presence of marine glycosides. The excimer formation of pyrene, a hydrophobic fluorescent probe, was significantly decreased by triterpene glycosides. The increase of tryptophanyl residue fluorescence demonstrated a change of the Na+-K+-ATPase conformation after treatment with cytotoxic glycosides.

Original languageEnglish (US)
Pages (from-to)937-945
Number of pages9
JournalToxicon
Volume27
Issue number8
DOIs
StatePublished - Jan 1 1989

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Triterpenes
Poisons
Glycosides
Adenosine Triphosphatases
Rats
Brain
Protons
Membranes
Arrhenius plots
sodium-translocating ATPase
Phosphatidylcholines
Fluorescent Dyes
X-Ray Diffraction
Electron microscopy
Conformations
Electron Microscopy
Fluorescence
Cholesterol
Nuclear magnetic resonance
Crystalline materials

ASJC Scopus subject areas

  • Toxicology

Cite this

Physicochemical characteristics of interaction of toxic triterpene glycosides from holothurians with rat brain Na+-K+-ATPase. / Gorshkova, I. A.; Kalinovsky, A. I.; Ilyin, S. G.; Gorshkov, B. A.; Stonik, V. A.

In: Toxicon, Vol. 27, No. 8, 01.01.1989, p. 937-945.

Research output: Contribution to journalArticle

Gorshkova, I. A. ; Kalinovsky, A. I. ; Ilyin, S. G. ; Gorshkov, B. A. ; Stonik, V. A. / Physicochemical characteristics of interaction of toxic triterpene glycosides from holothurians with rat brain Na+-K+-ATPase. In: Toxicon. 1989 ; Vol. 27, No. 8. pp. 937-945.
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