Abstract
Hybrids formed between human α and β globin cDNA and total human cellular DNA have been studied by thermal denaturation and cesium chloride density gradient centrifugation. From these studies, the weight average G+C content of human α globin cDNA has been determined to be 62%±2% and that of human β globin cDNA 51%±2%. These values correlate well with the results of G+C content of the human α and β globin cDNAs as determined by direct nucleotide sequence analysis of the cDNAs. Thermal denaturation and cesium chloride density gradient centrifugation of DNA-cDNA hybrids can therefore provide accurate information on the base composition of mRNA related sequences of any single copy gene for which a relatively pure cDNA can be obtained, without the necessity for direct nucleotide sequence analysis.
Original language | English (US) |
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Pages (from-to) | 179-183 |
Number of pages | 5 |
Journal | Molecular Biology Reports |
Volume | 6 |
Issue number | 3 |
DOIs | |
State | Published - Sep 1980 |
Externally published | Yes |
ASJC Scopus subject areas
- Molecular Biology
- Genetics