Physicochemical measurement of the base composition of mRNA-related sequences of the human α and β globin genes

Dorothy Tuan Lo, Bernard G. Forget

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Hybrids formed between human α and β globin cDNA and total human cellular DNA have been studied by thermal denaturation and cesium chloride density gradient centrifugation. From these studies, the weight average G+C content of human α globin cDNA has been determined to be 62%±2% and that of human β globin cDNA 51%±2%. These values correlate well with the results of G+C content of the human α and β globin cDNAs as determined by direct nucleotide sequence analysis of the cDNAs. Thermal denaturation and cesium chloride density gradient centrifugation of DNA-cDNA hybrids can therefore provide accurate information on the base composition of mRNA related sequences of any single copy gene for which a relatively pure cDNA can be obtained, without the necessity for direct nucleotide sequence analysis.

Original languageEnglish (US)
Pages (from-to)179-183
Number of pages5
JournalMolecular Biology Reports
Volume6
Issue number3
DOIs
StatePublished - Sep 1 1980
Externally publishedYes

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Globins
Base Composition
Complementary DNA
Messenger RNA
Genes
Density Gradient Centrifugation
Sequence Analysis
Hot Temperature
DNA
Weights and Measures

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Physicochemical measurement of the base composition of mRNA-related sequences of the human α and β globin genes. / Tuan Lo, Dorothy; G. Forget, Bernard.

In: Molecular Biology Reports, Vol. 6, No. 3, 01.09.1980, p. 179-183.

Research output: Contribution to journalArticle

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