Phytochemical constituents and free radical scavenging activity of Clerodendron glandulosum.Coleb methanolic extract

Ravirajsinh Jadeja, Menaka Thounaojam, Ansarullah, A. V. Ramachandran, Ranjitsinh Devkar

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Objective: The present study was undertaken to evaluate the phytochemical constituents and antioxidant and free radical scavenging properties of Clerodendron glandulosum.Coleb. Methods: - Methanolic extract of C.glandulosum.Coleb (MECG) was studied for its qualitative and quantitative phytochemical constituents and free radical scavenging potential using different in vitro assays for hydrogen peroxide, hydroxyl, superoxide, DPPH, nitric oxide, peroxynitrite, singlet oxygen and hypochlorous acid radicals scavenging assays. Its lipid peroxidation inhibitory activity, metal chelating activity and reducing power were also assayed. Results: Qualitative phytochemical screening of MECG showed presence of polyphenols, steroids, flavanoids and saponins. Quantitative phytochemical analysis revealed 34.3±0.89 mg/ml gallic acid equivalent-polyphenols, 46.1±1.00 mg/ml quercetin equivalent-flavanoids and 53.36±0.93 mg/ml ascorbic acid per 100 mg MECG. The MECG scavenges hydrogen peroxide (IC50 120.23±1.53 μg/ml), hydroxyl (IC50 70.23±1.36 μg/ml) superoxide (IC50 80.36±1.36 μg/ml), DPPH (IC50 50.11±1.36 μg/ml) and nitric oxide (IC50 90.11±1.55 μg/ml) radicals in a dose dependent manner. The IC50 values for peroxynitrite, singlet oxygen and hypochlorous acid were 48.41±1.72, 62.15±1.69 and 136.69±2.01 μg/ml, respectively. MECG also inhibited lipid peroxidation (IC50 150.56±3.02 μg/ml) and promoted metal chelation (IC50 50.29±2.00 μg/ml) in a dose-dependent manner. Assay of reducing capacity of MECG showed a dose-dependent response. Conclusion: The results suggest that a methanolic extract of Clerodendron glandulosum.Coleb possesses a strong antioxidant activity against all known radicals and can be considered as a natural antioxidant.

Original languageEnglish (US)
Article number19
JournalJournal of Complementary and Integrative Medicine
Volume6
Issue number1
DOIs
StatePublished - Aug 18 2009
Externally publishedYes

Fingerprint

Clerodendrum
Phytochemicals
Inhibitory Concentration 50
Free Radicals
Hypochlorous Acid
Singlet Oxygen
Peroxynitrous Acid
Antioxidants
Polyphenols
Superoxides
Hydroxyl Radical
Hydrogen Peroxide
Lipid Peroxidation
Nitric Oxide
Metals
Gallic Acid
Quercetin
Saponins
Ascorbic Acid
Steroids

Keywords

  • Clerodendron glandulosum.Coleb
  • Free radical
  • In vitro antioxidant

ASJC Scopus subject areas

  • Medicine(all)
  • Complementary and alternative medicine

Cite this

Phytochemical constituents and free radical scavenging activity of Clerodendron glandulosum.Coleb methanolic extract. / Jadeja, Ravirajsinh; Thounaojam, Menaka; Ansarullah; Ramachandran, A. V.; Devkar, Ranjitsinh.

In: Journal of Complementary and Integrative Medicine, Vol. 6, No. 1, 19, 18.08.2009.

Research output: Contribution to journalArticle

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AU - Devkar, Ranjitsinh

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