piggyBac is a flexible and highly active transposon as compared to Sleeping Beauty, Tol2, and Mos1 in mammalian cells

Sareina Chiung Yuan Wu, Yaa Jyuhn James Meir, Craig J. Coates, Alfred M. Handler, Pawel Pelczar, Stefan Moisyadi, Joseph Michael Kaminski

Research output: Contribution to journalArticle

232 Citations (Scopus)

Abstract

A nonviral vector for highly efficient site-specific integration would be desirable for many applications in transgenesis, including gene therapy. In this study we directly compared the genomic integration efficiencies of piggyBac, hyperactive Sleeping Beauty (SB11), Tol2, and Mos1 in four mammalian cell lines. piggyBac demonstrated significantly higher transposition activity in all cell lines whereas Mos1 had no activity. Furthermore, piggyBac transposase coupled to the GAL4 DNA-binding domain retains transposition activity whereas similarly manipulated gene products of Tol2 and SB11 were inactive. The high transposition activity of piggyBac and the flexibility for molecular modification of its transposase suggest the possibility of using it routinely for mammalian transgenesis.

Original languageEnglish (US)
Pages (from-to)15008-15013
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume103
Issue number41
DOIs
StatePublished - Oct 10 2006

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Transposases
Beauty
Gene Transfer Techniques
Cell Line
Genetic Therapy
DNA
Genes

Keywords

  • Cancer
  • Gene therapy
  • Mutagenesis
  • Site-specific
  • Transposase

ASJC Scopus subject areas

  • General

Cite this

piggyBac is a flexible and highly active transposon as compared to Sleeping Beauty, Tol2, and Mos1 in mammalian cells. / Wu, Sareina Chiung Yuan; Meir, Yaa Jyuhn James; Coates, Craig J.; Handler, Alfred M.; Pelczar, Pawel; Moisyadi, Stefan; Kaminski, Joseph Michael.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, No. 41, 10.10.2006, p. 15008-15013.

Research output: Contribution to journalArticle

Wu, Sareina Chiung Yuan ; Meir, Yaa Jyuhn James ; Coates, Craig J. ; Handler, Alfred M. ; Pelczar, Pawel ; Moisyadi, Stefan ; Kaminski, Joseph Michael. / piggyBac is a flexible and highly active transposon as compared to Sleeping Beauty, Tol2, and Mos1 in mammalian cells. In: Proceedings of the National Academy of Sciences of the United States of America. 2006 ; Vol. 103, No. 41. pp. 15008-15013.
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AU - Handler, Alfred M.

AU - Pelczar, Pawel

AU - Moisyadi, Stefan

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