Piperine suppresses cerebral ischemia-reperfusion-induced inflammation through the repression of COX-2, NOS-2, and NF-κB in middle cerebral artery occlusion rat model

Kumar Vaibhav, Pallavi Shrivastava, Hayate Javed, Andleeb Khan, Md Ejaz Ahmed, Rizwana Tabassum, Mohd Moshahid Khan, Gulrana Khuwaja, Farah Islam, M. Saeed Siddiqui, Mohammed M. Safhi, Fakhrul Islam

Research output: Contribution to journalArticle

65 Scopus citations

Abstract

The pathophysiological mechanisms leading to neuronal injury in middle cerebral artery occlusion (MCAO) model of cerebral stroke are complex and multifactorial that form the bases of behavioral deficits and inflammation mediated damage. The present study demonstrates the effect of piperine pretreatment (10 mg/kg b wt, once daily p.o. for 15 days) on cerebral ischemia-induced inflammation in male Wistar rats. The right middle cerebral artery was occluded for 2 h followed by reperfusion for 22 h. A maximum infarct volume (57.80 %) was observed in ischemic MCAO group. However, piperine administration prior to ischemia showed a significant reduction in infarct volume (28.29 %; p < 0.05) and neuronal loss (12.72 %; p < 0.01). As a result of piperine pretreatment, a significant improvement in behavioral outputs of MCAO rats (p < 0.05-0.01) was observed. Piperine successfully reduced the level of proinflammatory cytokines IL-1β, IL-6 and TNF-α, in ischemic group (p < 0.01). Ischemic group brain has shown edematous morphology with vacuolated architecture and pyknotic nuclei in H & E staining which was successfully ameliorated by piperine administration. Moreover, piperine also succeeded in lowering the expression of COX-2, NOS-2, and NF-κB (p < 0.01). Both cytosolic and nuclear NF-κB were down-regulated in ischemic group pre-administered with piperine (p < 0.01). The present study suggests that piperine is able to salvage the ischemic penumbral zone neurons by virtue of its anti-inflammatory property, thereby limiting ischemic cell death.

Original languageEnglish (US)
Pages (from-to)73-84
Number of pages12
JournalMolecular and Cellular Biochemistry
Volume367
Issue number1-2
DOIs
StatePublished - Aug 1 2012

Keywords

  • Behavioral deficit
  • COX-2
  • MCAO
  • NF-κB
  • NOS-2
  • Piperine

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

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  • Cite this

    Vaibhav, K., Shrivastava, P., Javed, H., Khan, A., Ahmed, M. E., Tabassum, R., Khan, M. M., Khuwaja, G., Islam, F., Saeed Siddiqui, M., Safhi, M. M., & Islam, F. (2012). Piperine suppresses cerebral ischemia-reperfusion-induced inflammation through the repression of COX-2, NOS-2, and NF-κB in middle cerebral artery occlusion rat model. Molecular and Cellular Biochemistry, 367(1-2), 73-84. https://doi.org/10.1007/s11010-012-1321-z